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THE EFFECT OF CATERPILLAR FUNGUS(Cordyceps sinensis [Berk.] Sacc.)

TOWARDS IL-2 LEEL INPARACETA!OL-INDUCED !ICE

(Mus musculusL.)

"OHANES IRSAND"#$%#%#$

FACULT" OF !EDICINE!ARANATHA CHRISTIAN UNIERSIT"

BANDUNG2##&

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BAC'GROUND

Paracea*+ A,a+e/c0 A,/1/re/c0 a,

A,//,3+aa/*,

S/e e33ec 4e1a**5/c/6

L*, er 7ae *3 1aracea*+ a6

ca7e +/8er aae

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BAC'GROUND c*,Paracea*+

N-acetyl-p-N-acetyl-p-benzoquinonebenzoquinone

imine (NAPQI)imine (NAPQI)

Biotransformation

Cytochrom P450(Van de straat et al.,

1987)

Very reactive &

Toic s!"stance

#i$id Peroidationreaction(%anson, 199)

Free radicalsFree radicals

LI!" #A$A%!

&' cells &' cells

IL- IL- IL-* IFN- &NF- '#4 '#5 '# '#10 '#1*

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BAC'GROUND c*,

Cordyceps sinensis/ *,e *3 a ra//*,a+4er9a+ e/c/,e0 :4/c4 4a 9ee, :/e+6 7e96 4e a,c/e, c4/,ee

Cordyceps sinensis(&%;0&2 )

Cordyceps sinensis7e a a+er,a/8e 4era163*r a,6 k/, *3 /eae (!a,*,0 %&&2)

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PROBLE!S

D*e Cordyceps sinensisecreaeer7 IL-2 +e8e+ /, 1aracea*+-/,7ce/ce

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OB?ECTIE

E5a/,e 4e e33ec *3 Cordyceps

sinensisaa/, er7 IL-2 +e8e+ /,1aracea*+-/,7ce /ce

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RESEARCH BENEFICIALS

Acae/c 9e,e3/c/a+@ * e51a, 4ek,*:+ee a9*7 4er9a+ e/c/,e01ar/c7+ar+6 Cordyceps sinensis

Prac/ca+ 9e,e3/c/a+@ * e51+*rec/e,/3/c a11+/ca/*, 3*r Cordycepssinensisa 4e1a*1r*ec*r

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FRA!E OF WOR'SParacea*+

er7IL-2 +e8e+

er7IL-2 +e8e+

L/8er Daae

Cordyceps sinensis

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H"POTHESIS

Cordyceps sinensisecreae er7 IL-2+e8e+ /, 1aracea*+-/,7ce /ce

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EPERI!ENTAL DESIGN

C*1ara/8e 1r*1ec/8e +a9*ra*r6e51er/e, :/4 c*1+ee ra,*/e e/,

!ea7ree, *3 er7 IL-2 +e8e+ ELISAe4*

Daa O,e-Wa6 ANOA = T7ke6 HSD

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TI!E AND PLACE

T/e @ ?7,e 2##> ?a,7ar6 2##&

P+ace @ P7a Pe,e+//a, I+7 'e*kera, (PPI')0

Fac7+6 *3 !e/c/,e-!ara,a4a C4r//a,U,/8er/60 Ba,7,

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!ACROSCOPIC OF LIER

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!ICROSCOPIC OF LIER

Ce,ra+8e/,

S/,7*/

S1a/7

D/e

Ter/,a++/3a/c

L/3a/c 8e/,

L/8er ce++

'7133er ce++

B/++/ar/ ca,a+/c7+/

P*ra+ 8e/,

He1a/c arer6

B/++/ar/ D7c

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1. Metabolic function

2. Haematologic function

3. Bile secetion

LIER FUNCTION

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1. !egeneation an" accumulation intacellula

2. #ecosis an" a$o$tosis

3. %nflammation

4. &egeneation

5. 'ibosis

6. (i)osis

LIER PATHOLOG"

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PARACETA!OL(Ace4a/,*14e,)

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INTERLEU'IN 2 (IL-2)

9+a*e,/c 3ac*r0 T ce++ r*:4 3ac*r0+614*c6e 1r*+/3era/, 3ac*r

'unction* T ce++ r*:4 3ac*r

S/7+ae N' ce++ = L/3*/ B 1r*+/3era/*,

N' ce++ /33ere,/a/*,

/,creae N'ce++ k/++/, e33ec

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BIOLOGIC ACTIIT"

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CATERPILLAR FUNGUS+Cordyceps sinensis ,Be-. /acc.

!iisio * 'ungi

/ub"iisio * scomcota

(lass * /o"aiomcetes "o * H$oceales

'amili * (laici$itaceae

enus * (o"ce$s /$ecies * Cordyceps sinensis +Be-. /acc.

(H*+/a6 a, C+ea8er0 2##)

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(% (M:#!/

; co"ce$in +3

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(o"ce$in +3

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!ATERIAL = !ETHODS

DD" /ce0 2#-2$ ra0 > :eek *+

A++ /ce :ere aa1e 3*r ; a6

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Gr*71@%. C!C % (#0L

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A8erae *3 Ser7 IL-2 Le8e+ a*, Gr*71Treae,

Groups erum IL-

Le.el

#ay / #ay

Negative Control 0 1,296

Paracetamol 19,515 40,979

Cordyceps sinensis 0,986 3,819

Paracetamol +Cordyceps sinensis

22,367 3,681

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Paracea*+ /,creae er7 IL-2 +e8e+ a Da6 $

#

2#

#

#

>#

C*,r*+ Paracea*+ CS Paracea*+ J

CS

D-$

IL-2Le8e+(1

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Cordyceps sinensis ecreae er7 IL-2 +e8e+ a Da6 %2

KK P #0### KK P #0###

KK P #0###

#

2#

#

#

>#

'*,r*+ Paraea*+ CS Paraea*+ J

CS

D-%2

IL-2Le8e+(1

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DISCUSSION

I, 1aracea*+-/,7ce /ce IL-2+e8e+ /,creae /,/3/ca,+6

Cordyceps sinensis (&%;

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DISCUSSION c*,

I,3+aa/*,

N' ce++

IL-2

CS

TNF-M

LIER DA!AGE

Paracea*+

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C*,c+7/*,

Cordyceps sinensis4a a, a9/+/6 *ecreae er7 IL-2 +e8e+in

$aracea*+-/,7ce /ce

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THAN' "OU

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