Pagina 1 - Curriculum vitae di MANARA, Elena

F O R M A T O E U R O P E O P E R I L C U R R I C U L U M

V I T A E

PERSONAL INFORMATION

Name MANARA ELENA Address CORSO STATI UNITI, 4 35127 PADOVA (PD)

Telephone +390498215488 Fax

E-mail Elena.manara@unipd.it

Nationality Italiana I

Date of birth 30/04/1981

WORK EXPERIENCE

8/2013-now Employed as a Post Doc at the Istituto di Ricerca Pediatrico in Padova. 8/2011-8/2013 Post Doc Research fellowship at Onco-hematology laboratory, Department of Pediatrics,

University of Padova, in the project “Ruolo di ICER nella leucemia acuta mieloide”. 8/2010-7/2011 European Research fellowship at Onco-hematology laboratory, Department of Pediatrics,

University of Padova, in the MONAD project “Engineering of molecular motors and cytoskeletal filaments in a nano device for ultrasensitive diagnostic disease markers”.

10/2008-7/2010 visiting PhD student at UCLA, Los Angeles, CA in the laboratory of Professor Sakamoto KM department of Pediatrics Hemato-oncology, works on a project regarding the regulation of CREB by miRNA in a mouse model.

01/2007-10/2008 PhD student at the University of Padova, Italy, works on a project regarding CREB and acute pediatric leukemia.

02/2006-12/2006

Fellowship “Città della Speranza” at the Onco-hematology laboratory of Professor Basso G, Department of Pediatrics, University of Padova, for the project: “Diagnostica per leucemie, linfomi non Hodgkin e non, sarcomi delle parti molli”.

09/2004-10/2005 training in the CRIBI laboratory of Professor Lanfranchi G, Department of Biology, University of Padova, for the thesis “Analisi comparativa dei dati di espressione genica prodotti con la tecnologia dei microarray di DNA”.

09/2004-10/2005 training in the CRIBI laboratory of Professor Lanfranchi G, Department of Biology, University of Padova, for the thesis “Analisi comparativa dei dati di espressione genica prodotti con la tecnologia dei microarray di DNA”.

05/2003-09/2003 training at the Department of Organic Chemistry in the laboratory of Professor Licini G, University of Padova, for the thesis “N-o-NBS-aminoacidi α,α-disostituiti: studio della reazione di formazione del legame amidico”.

EDUCATION

2007-2010 PhD in “Developmental medicine and programming of science”, University of Padua, Italy 2000-2005 Magna cum laude. Bachelor in Sciences, Industrial Biotechnology, University of Padua, Italy 1998-1999 AFS program: senior year at the Mckinleyville high school, Mckinleyville, CA.

Pagina 2 - Curriculum vitae di MANARA, Elena

NATIVE LANGUAGE ITALIAN

OTHER ENGLISH

• Reading Excellent • Writing Excellent

• Speech Very Good

EXPERTISE

- Molecular biology techniques: PCR and RealTimePCR, DNA and RNA electrophoresis, Sanger sequencing, DNA RNA and protein extraction, western blot, immunoprecipitation, plasmid construction and recombination, immunofluorescence, immunohistochemistry. - Cellular biology techniques: cell line culture, primary culture, transfection techniques, drug treatment, bacteria culture and transformation, flowcitometry (cell cycle and annexinV/PI assay). - In vivo models: mice: intraperitoneal, subcutaneous and tail vein injections, bone marrow transplant, sacrifice.

Pagina 3 - Curriculum vitae di MANARA, Elena

PUBLICATIONS • Manara E, Bisio V, Masetti R, Beqiri V, Rondelli R, Menna G, Micalizzi C,Santoro N, Locatelli F, Basso G, Pigazzi M. Core-binding factor acute myeloidleukemia in pediatric patients enrolled in the AIEOP AML 2002/01 trial: screeningand prognostic impact of c-KIT mutations. Leukemia. 2013 Nov 14. doi:10.1038/leu.2013.339.

• Masetti R, Togni M, Astolfi A, Pigazzi M, Manara E, Indio V, Rizzari C,Rutella S, Basso G, Pession A, Locatelli F. DHH-RHEBL1 fusion transcript: a novelrecurrent feature in the new landscape of pediatric CBFA2T3-GLIS2-positive acute myeloid leukemia. Oncotarget. 2013 Oct;4(10):1712-20.

• Masetti R, Pigazzi M, Togni M, Astolfi A, Indio V, Manara E, Casadio R,Pession A, Basso G, Locatelli F. CBFA2T3-GLIS2 fusion transcript is a novelcommon feature in pediatric, cytogenetically normal AML, not restricted to FAB M7subtype. Blood. 2013 Apr 25;121(17):3469-72. doi: 10.1182/blood-2012-11-469825.

• Pigazzi M, Manara E, Bisio V, Aveic S, Masetti R, Menna G, Zecca M, Pession A,Locatelli F, Basso G. Screening of novel genetic aberrations in pediatric acutemyeloid leukemia: a report from the AIEOP AML-2002 study group. Blood. 2012 Nov1;120(18):3860-2. doi: 10.1182/blood-2012-09-454454.

• Pigazzi M*, Manara E*, Bresolin S, Tregnago C, Beghin A, Baron E, Giarin E, Cho EC, Masetti R, Rao DS, Sakamoto KM, Basso G. MicroRNA-34b promoterhypermethylation induces CREB overexpression and contributes to myeloidtransformation. Haematologica. 2013 Apr;98(4):602-10. * Co-first

• Sandoval S, Kraus C, Cho EC, Cho M, Bies J, Manara E, Accordi B, Landaw EM,Wolff L, Pigazzi M, Sakamoto KM. Sox4 cooperates with CREB in myeloidtransformation. Blood. 2012 Jul 5;120(1):155-65. doi:10.1182/blood-2011-05-357418.

• Pigazzi M, Masetti R, Martinolli F, Manara E, Beghin A, Rondelli R, Locatelli F, Fagioli F, Pession A, Basso G. Presence of high-ERG expression is anindependent unfavorable prognostic marker in MLL-rearranged childhood myeloidleukemia. Blood. 2012 Jan 26;119(4):1086-7; author reply 1087-8. doi:10.1182/blood-2011-10-385815. PubMed PMID: 22282493.

• Pigazzi M, Manara E, Beghin A, Baron E, Tregnago C, Basso G. ICER evokesDusp1-p38 pathway enhancing chemotherapy sensitivity in myeloid leukemia. ClinCancer Res. 2011 Feb 15;17(4):742-52. doi: 10.1158/1078-0432.CCR-10-0886.

• Pigazzi M, Manara E, Baron E, Basso G. miR-34b targets cyclic AMP-responsiveelement binding protein in acute myeloid leukemia. Cancer Res. 2009 Mar15;69(6):2471-8. doi: 10.1158/0008-5472.CAN-08-3404.

• Pigazzi M, Manara E, Baron E, Basso G. ICER expression inhibits leukemiaphenotype and controls tumor progression. Leukemia. 2008 Dec;22(12):2217-25. doi:10.1038/leu.2008.244.