DigiBio Biologia Digitale: il nesso tra memoria dell’acqua, pratiche alternative e medicina del...

DigiBio

Biologia Digitale: il nesso tra memoria dell’acqua, pratiche alternative e

medicina del futuro

Jacques Benveniste, MD, Directeur de Recherche

Didier Guillonnet, MS Informatique

v 1.4 US

www.digibio.com.

Centro Congressi CavourRoma, 14 dicembre 2002

Professor Benveniste, why is it so difficult for you to be accepted by the system?

Drawing by JP Petitin "H2O"Science Innovante publisher

DigiBiowww.digibio.com

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History

Generalities

G.1b

The high dilution story (dubbed the "Memory of Water")

1984 Basophil degranulation triggered by high dilution of serum anti-IgE.

1988 Publication in Nature, followed by an ‘‘inquiry’’.

1991 Erasing of high dilution activities by an oscillating magnetic field.(collaboration with a CNRS team)

1992 Electronic transfer (via an amplifier) of biological information to a tube of water.

1995 Digitization: recording then replay of the biological message using a computer.

1998 Activation by agitation of a solution at very low concentration (down to 10-14M)


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Technologies

T.1.2

- Biological Sensitive System - Langendorff


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Granules Mean + 1 SD (n)Arnica montana 15CH 16.0 + 1.2 (4)* Arnica montana 30CH 13.7 + 8.7 (3)*Inert 3.6 + 3.0 (4)

Digitally-Recorded Granules (d)d-Arnica montana 15CH 19.4 + 3.3 (5)**d-Arnica montana 30CH 14.4 + 1.4 (3)**d-Inert 2.9 + 0.8 (3)

Arnica montana MT (1/106) 29.9 +10.6 (3) * p < 0.05 compared with inert granules ** p < 0.05 compared with d-inert granules c DigiBio 1999

EFFECT OF HOMEOPATHIC GRANULES (ARNICA montana) ON ISOLATED GUINEA-PIG HEARTS

(% increase in coronary flow)


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BIOLOGICAL ACTIVITY IN HOMEOPATHIC GRANULES (Arnica montana)

(Guinea-pig isolated heart - % var. in coronary flow)

Ethylicum Arnica mont. Open/ Date ofDate 15CH 15CH Blind purchase

18.9.98 4.8 13.8 Open June 98

21.9.98 5.4 13.5 Open June 98

24.9.98 5.4 19.1 Blind June 98

25.9.98 4.8 18.4 Blind Sept 98

30.9.98 5.6 2.9 25.0 22.9 Blind Sept 98

1.10.98 3.1 9.3 18.2 15.6 21.2 Blind Sept 98

5.10.98 4.0 20.0 Blind Sept 98


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EFFECTS OF HOMEOPATHIC GRANULES (ARNICA MONTANA) ON SKIN GUINEA-PIG

(diameters of blue dots in mm)

Mean + 1 SD (n)

Arnica montana 15CH 7,4,7,7,12,8 7.5 + 2.6 (6)*

Arnica montana 30CH 16,12,18,20,17 16.6 + 2.9 (5)*

Inert 2, 0, 0, 1, 0 0.6 + 0.8 (5)

____________________________________________________

Statistically significant diff. (Student’s t-test for unpaired variates):

* p < 0.05 compared with inert granules


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Technologies

T.1.5

- Biological Sensitive System -

Skin Test

1 ACh -12 M vortexed in saline (68.2) 7 ACh signal in water (1,294)2 Same vortexed in 5 % glucose (59.5) 8 Same at low power (435)3 Same vortexed in water (1,949) 9 Same as 6 (987)4 Same mixed in water (44) 10 Acetate+Choline as in 3 (25)5 Atropine + 3 (71) 11 ACh 1 µg (1,154)6 Water + 3 (609) 12 Atropine + ACh 1 µg (36)

Results in pixels x 10 3


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THE ESSENTIAL ROLE OF WATER

IN THE TRANSMISSION OF THE

MOLECULAR SIGNAL

*

EFFECT OF VIOLENT AGITATION


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- Biological Sensitive System - Skin Test

Technologies

T.1.2.2

EFFECT ON GUINEA-PIG SKIN OF Vortexed vs Mixed

ACETYLCHOLINE (1pM) or ACETATE-CHOLINE (1pM)

21/10/98

ACh 1pM (mixed) 1.6 + 0.8 (11)

ACh 1pM (vortexed) 12.9 + 4.9 (17)*

AC 1pM (vortexed) 1.3 + 0.8 (9)

AC 1pM (mixed) 1.4 + 0.5 (7)

ACh 1µM 18.3 + 4.7 (11)

Diam. of blue dots in mm ; mean + 1 SD (n)

* p < 0.05 compared with ACh 1pM mix. and AC 1pM mix. or vor.


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Technologies

T.1.5

- Biological Sensitive System -

Skin Test

1 ACh -12 M vortexed in saline (68.2) 7 ACh signal in water (1,294)2 Same vortexed in 5 % glucose (59.5) 8 Same at low power (435)3 Same vortexed in water (1,949) 9 Same as 6 (987)4 Same mixed in water (44) 10 Acetate+Choline as in 3 (25)5 Atropine + 3 (71) 11 ACh 1 µg (1,154)6 Water + 3 (609) 12 Atropine + ACh 1 µg (36)

Results in pixels x 10 3


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Skin Testing in the G-P: Effect of Dilution and Digital Signals

(Comments on the June 5, 1998 Experiment)

The experiment presented here shows that a specific biological activity can be obtained by “informing” water. It sums up a body of research which demonstrates: 1) the existence of biologically-specific signals, in the form of electromagnetic waves; 2) the role of water in the transmission and “memorization” of these signals.

The experimental procedure has two aspects: 1) the activation, by simple mechanical agitation, of a molecular solution at a normally inactive concentration (1 x 10-12 M); this agitation reveals an activity in all ways comparable to that obtained with a typical active concentration (1 x 10-6 M); 2) using an electromagnetic coil, transmission to “naive” water of a specific electromagnetic signal pre-recorded on a computer.


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Technologies

T.1.2


WATER IS ESSENTIALIN TRANSFERRING THE MOLECULAR SIGNAL

% increase in coronary flow

Signal recorded from:

ACh 1 µM

in 100% ethanol, VORTEXED 4.3 ± 1.8 (3)

in 80% ethanol / 20% water, MIXED 8.0 ± 1.0 (3)

in 80% ethanol / 20% water, VORTEXED 22.7 ± 3.5 (3) *

* p < 0.05 compared with 100% ethanol

mean + 1 SD (n)


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- Biological Sensitive Systems -Operating modes

Technologies

T.3

MIMICKING THE SPECIFIC

EFFECTS OF MOLECULES WITH

COMPUTER-RECORDED SIGNALS

(Low e.m. frequencies)


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Technologies

T.5a

White noiseSignal modified by

the sample

Signal recording

Tube containing the substance to be recorded

Electromagnetic coils

External EM fieldshielding container

Emitter Receiver


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Technologies

T.1.2.2

d-Caffeine 15.7 ± 4.9 (13)

Caffeine (1µM): 22.3 ± 2.3 (3)

d-Water (water recorded as control) 4.6 ± 1.4 (6)

EFFECTS ON ISOLATED GUINEA-PIG HEARTS OF THE

DIGITALLY-RECORDED (d) CAFFEINE% DECREASE in coronary flow. Mean + 1 SD (n)

14/10/98


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The biological effect is similar whether it’s from a physical molecule or its signal obtained by HD


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Technologies

T.1.2.2

Iono 1pM 3.6, 5.1, 5.1, 4.6 4.6 ± 0.3 (4) (mixed)

Iono 1pM 13.0, 42.9, 37.5, 11.1, 14.8 23.8 ± 6.7(5)(vortexed)

Iono 1 µM 39.6, 40.0, 50.0 43.2 ± 3.4 (3)

EFFECTS ON ISOLATED GUINEA-PIG HEARTS OF THE

DIGITALLY-RECORDED (d) IONOPHORE (1pM)(Vortexed vs Mixed)

% increase in coronary flow mean + 1 SD (n)


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Technologies

T.1.6.4Blood coagulation results

Interaction of a person(whole body? Hands? Mind?)

with the heparin signal as shown by «Sandwich» experiments

2) The exposed water-Ca2+ tubes are mixed with plasma and distributed in 96-well plates, first by an operator (JA), then by a second operator (SKL), then again by JA.

1) Ca2+-containing water is exposed to the heparin (or water as control) signal.


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Automatic Bio Analyser


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1=HEP 2=EAU 3=EAU 4=HEP

PPP congelé - Mouton 14Inhibition de coagulation

EFFECT OF HEPARIN SIGNALBlind - January 20, 2000


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EFFECT OF HEPARIN SIGNALBlind - January 20, 2000

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PPP congelé - Mouton 14Inhibition de coagulation


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Blood coagulation results

When SLK processed the tubes that were first found active in the hands of JA,

the activity disappeared, most often definitively. Subsequent expts showed that

this negative influence could be exerted from up to 1 meter distance (not shown).

We have located another person with similar activity. At this time (Oct. 2000), we

do not know the frequency of these « erasers » in the general population. Nor do

we know whether or not there exists a link between this effect and other

documented « paranormal » abilities.

This method provides a pharmacological tool to routinely investigate such

phenomena.

Interaction of a person with the heparin signal:Results and Discussion

Technologies

T.1.6.7


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Technologies

T.1.6.6Blood coagulation results

« Sandwich » experiments

Expt.Nb. Wa* He He/Wa Wa He He/Wa Wa He He/Wa

1 529 80 15,1% 573 548 95,6% N.A. N.A. N.A.

2 561 186 33,2% 543 565 104,1% N.A. N.A. N.A.

3 569 260 45,6% 633 653 103,2% 290 303 104,5%

4 736 39 5,3% 740 654 88,4% 722 26 3,6%

5 795 118 14,8% 770 759 98,6% 740 789 106,6%

Mean 638 137 22,8% 652 636 98,0% 350 224 42,9%

JA 1 SKL JA 2

* results (O.D. at 40 min) are means of duplicates


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Coherent domains in WaterG. Preparata & E. Del Giudice


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TheoreticalConsiderations

P.1Molecular signalling

The 3D structure of the ligand matches that of the receptor. Physical proximity induces receptor conformational changes which in turn triggers the cascade of events prompting cell function.

The Current Theory:"structural matching"

The Proposed Theory:"electromagnetic signals"

Proximity favors resonance of ligand-receptor specific EM signals. Resonance serves as an amplifying mechanism for conformational changes which occur at all steps of the cascade inducing cell function.


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Conclusion (1)(Homeopathy)

High dilutions share the same mechanismwith usual biology:

the molecular signal (hertzian waves) is carriedby water to its target whether or not the sourcesubstance is still present. How water supports the signal is unknown.

Homeopathy made an empirical usage of this fundamental property of molecules:

Applying the methods of modern pharmacologyallows us to detect the specific signal present inhomeopathic preparations.


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Conclusion (2)(Homeopathy)

These advances permit to envision:

- the implementation of a remote digital quality control aimed at insuring the actual presence of therapeutic activity in homeopathic products.

- the future manufacturing of purely digital homeopathic products.


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Conclusion(General)

• We can trigger specific biological effects with low frequency electromagnetic signals.

• These biological signals are carried/transmitted by water.

• They seem to support homeopathic activity.

• They are sensitive to the influence of some individuals.

• These influences may account for the difficulty in consistently replicating experimental systems, more than intrinsic variability of the latter.


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More Conclusions and a Question

The experiments presented here show unequivocally that

the mechanisms instrumental in homeopathy do belong to

the realm of contemporary physics and biology. Therefore

they can be investigated by usual scientific procedures.

QUESTION: How can we explain that, homeopathic

practices being under world-wide attacks (mainly based on

the lack of scientific grounds) from « mainstream » science,

no efforts are made by the industry and the practitioners to

use a small percentage of their income to support

fundamental research in homeopathy?

TENTATIVE ANSWER: a suicidal attitude?


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Please check this reference

Activation of human neutrophils by electonically transmitted phorbol-myristate acetate

Y. Thomas, M. Schiff, L Belkadi, P. Jurgens, L. Kahhak,

J. Benveniste. Medical Hypotheses (2000) 54(1), 33-39

Reprints at <[email protected]> or <[email protected]>


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Jacques Benveniste• Exploration of new paths and deepening of the discovery• Also in charge of communication and external affairs• MD, Director of Research at INSERM - (National Medical Research Inst.)• Famous for the discovery of PAF (Platelet-Activating Factor)

Didier Guillonnet• Rationalization of protocols, Development of the automate, Signal processing• Also administration, management, legal affairs and intellectual property• MS Computer Science (Ecole Centrale de Paris)

Jamal Aissa (Ph.D), Larbi Kahhak (Ph.D), Soo K. Lim (MD, Ph.D) • Research Engineers in biology who have worked with J. Benveniste for many years,

performing various experiments such as “isolated guinea pig heart” and “cell culture”…

Business angels experienced in company creation from seed to stock market

A patent lawyer widely recognized for his expertise in cutting edge technologies

A complementary Team


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Jacques Benveniste [email protected]

Didier Guillonnet [email protected]

DigiBio S.A.34 av. des Champs Elysées 75008 Paris - France

Digital Biology Research Laboratory32 rue des Carnets, 92140 Clamart - France

Tel. +33 (0)1 46 01 58 40 Fax +33 (0)1 46 31 02 77

http://www.digibio.com

Contacts


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MOTTO

Los homeopatas unidos (y haciendoinvestigaciones) jamas seran

vincidos.

Pero no son unidos, hacen poca investigacion y cuando lo hacen,

publican en periodicos noncientificos o confidenciales.

DigiBio Biologia Digitale: il nesso tra memoria dell’acqua, pratiche alternative e medicina del...

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