CarloAlberto RediAccademia dei Lincei

Università degli Studi di PaviaFondazione IRCCS

Policlinico San Matteo - Paviac.redi@smatteo.pv.it

Il futurodelle

cellule staminali

Milano 16 luglio 2009Palazzo Besanaore 18.30

Diagramma di Valentin Häcker (1892)con cellule staminali nell’embrione di Cyclops

Diagramma di Artur Pappenheim (1905) per illustrare la ematopoiesi

Stem cell basic biology

neonato

adulto

Crescita e differenziamento

fecondazione

sviluppo embrionale

zigote

oocita

sperm

meiosi

meiosi

Lialm Donaldson report, June 2000

SCAFFOLDS, CELLULE STAMINALI EBIOREATTORI PER LA PRODUZIONE DITESSUTO OSSEO BIOINGEGNERIZZATO

F. Benazzo and the components of CIT

SEM 450 XAnti-osteocalcin

Punched Titanium

SEM 50 X

surviving graft 10 ye ars after transplantationof human fetal dopamine neurons to the right

striatum

Positron emission tomography scan Olle Lindvall, “Stem cell transplantation”

Variazione della captazione di 18F-fluorodopa nei cervelli di pazienticon malattia di Parkinson dopotrapianto, dimostrato dall‘esamePET

1 106

!! Cytoplast !!

!! Cytoplast !!

TELOMERETELOMERE’’ LENGTH AT DIFFERENT TIME LENGTH AT DIFFERENT TIMEPOINTS OF EXPANSIONPOINTS OF EXPANSION

WEEKS OFCULTURE

00 17.7 17.7 8.8 8.8+2+2 21.1 21.1 10.4 10.4

+4+4 20.7 20.7 10.2 10.2

+5+5 18.6 18.6 9.8 9.8

+6+6 18.4 18.4 9.1 9.1

KMESF KMESF KbKb

(FLOW-FISH)(FLOW-FISH)

Yamanaka and coworkers (2006/07): ≈1 on 5,000 ≈ 0.02% Thomson and coworkers (2007): ≈ 1 on 10,000 ≈ 0.01% cytoplasts (insulinoma & stem cell extracts)Collas and coworkers (2004): ≈1 on 2 ≈ 50%Redi and coworkers (2007): ≈ 1 on 3,000 ≈ 0.03%

retrotransfections (stemness & oncogenes)

! ! !biotechnology

cloningstem cells

NO

stem cells

objections to reproductivebiotech

We should not play God

Henrietta Lacks 1920 - 1951

HeLa cellsHeLa cells should be given the new speciesname of Helacyton gartleri.The cells are a genetic chimera of HPV18 andhuman cervical cells and now have a distinct,stable, non-human chromosome number.

HeLa cellsHeLa cancer cells have evolved into a self-replicating, single-cell life-form.

HeLa cellssince 1951 self-replicatingused in thousands biol expcontribution to medicine