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Ulteriori Ricerche SullaCostituzione del Nucleolo diPaphiopedilum Spicerianum(Rchb. f.) PfitzProf Eleonora FranciniPublished online: 14 Sep 2009.

To cite this article: Prof Eleonora Francini (1938) Ulteriori Ricerche Sulla Costituzionedel Nucleolo di Paphiopedilum Spicerianum (Rchb. f.) Pfitz, Giornale botanicoitaliano: Official Journal of the Societa Botanica Italiana, 45:3, 235-266, DOI:10.1080/11263503809438755

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Prof. ELEONORA FRANCINI -

ULTERIOR1 RICERCHE

SULLA. COSI'ITUZIONE D E L .NUCLEOLO

DI PAPHIOPEDlLUiM SPlCERlANURl (Rchb. f.) Pfitz. (COS TAV.' xv) (*)

SOJIJIAIiJO

ISTRO~LIZIOSE. . . . . . . . . . . . . . . . . prig. '336

I. - Prove con fissntivi diversi. 1. - Pl tOVl f W)L JIETODO DEL.\UXAY 5IOI~IYICATO.

Fissaziono dulln mctb di tin npice radicale in Dehitnay e post- crornizzaziotic ; fissnzione dcll' nltra rneth in Delniinny -c ncido acctico o postcrotiiizznzione . . . . . . . . 238

1. Fisnazioiie in 1)elnunny . . . . . . . . . - 233 2. Fissnzioric in Delnutiny + 6 '/, acido acetico. . . u 238

Fissazione dclln meth di 1111 npice rndicalc in Delnunny c post- croiiiizznzioiie ; fissnzione dell'altra rnetk in Dclauriay scnza postcromieznzioiio . . . . . . . . . . - . . s 939

1. Con croniizzazione. . . . . . . . . . . s 239 2. Sonzn cromizzriziorte . . . . . . . . . . s "39

znzioite. . . . . . . . . . ~ . . . . . . D 239 Fissnzione i n Delaunny con proliingalneiito della poutcromiz-

3. - P R O V E COS A L T I I I 31€;101)1 Il l YIJSAZIOSC.

Fissaziono Rcgaud . . . . . . . . . . . . . . s Cinccio . . . . . . . . . . . . . , - Cajal c DR Fnno . . . . . . . . . . . . Bends. . . . . . . , . . . . . . .

I. Pissnzione per 21 11. iicl liquido Bendn-JIeves . . 2. '. e croniizzazione secoiido Bendn . . . .

Fissazione Nnwnscliin . . . . . . . . . . . . . 8 in :ilcool nssoliito o 95" ., . . . . . . . . 8 Cnrnoy . . . . . . . . . . . . . . * in formolo . . . . . . . . . . . . . D i n picro-formolo . . . . . . . .. . . .

Fissntivi non acetici o.siicccssiro pn3sriggio i i i ncido acetico.

e) Dall'Istitiito Botanic0 dclla R. Ultiverjith d i Pi3a, diretto dal Prof. ALUERTO Crrra1:uor.

2351 [Nuovo Ciornale Botanlco Itallano, n. s., Vol. XLV, N. 3, 1938

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236 F R A S G I S I

11. - Prove speciali cou reattivi diverri. I . - PROVA rm I,’ACIDO TIMOSUCLGICO . . . . . 2. - pl;OVI: PEP. 1 I’ItOTIDI. .

Reszionc xnntoproteica . . . . . . . . .

Colorazione col nero .Sodan U. Netodo di Cinccio per i Iipoidi . . . . . . .

8 di Ciaccio pcr la lipofanerozi . . . . Renzioiie plasmale . . . . . . . . . .

8 di Axeiifcld . . . . . . . . . 3. - PI!OYE PEI? I Ll1’IUI.

. . . . . . s di Ciaccio per le fosfo- c Ic gnlacto-lipine

4. - PcovrP PER LU J1UCII.I.AUQISI. Virnggio mctncromatico del bleu di toluidinn. . Colorazioiie col row0 di rutenio . . . . . .

111. - Discussione . . . . . . . . . . . . Conclusioni . . . . . . . . . . . . . . Bibliogrnfin. . . . . . . . . . . . . . . . Spiegazione della Tavola S\’ . . . . . . . . Itinssunto . . . . . . . . . . . . . .

. . . . pag.

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. . . . 8

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. . I . )

. , . . 8

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218

249 24%

h i iiiicl. not;% prcccclcntc (k‘n.iscrs~, 1037) 110 riportato i priini ri- iii1ltitti di riccrclic! d : ~ RIC iriizi:itc sul iiuclco’lo tli Z’ni~liio~rcdiliit~~ Spiccricr 11 ~ 1 1 1 . E ~ : ~ ~ i i i ~ i i l i d ~ i l croriiportnriicntd dcl i~iiclcolo iii npici Tiiclicnli fiss3t.i coil iiii liquid0 ncctico, il liqiii(1o ili ~a rpc t sc~ icn~ ;o , NI iri npici i iwi ti scc:on(lo la tccnicn iiiitocoii(lri:ilc, coil il 11icto(10 cii Dclnuriny, ho rilci-nto die il fiss:itorc ncctico uicttc in cvidcnzn uri iiiicleolo sciiiplice, cornposto di tin? sostanz;i a., non colornbile coil cm:i tossiliiin di Ileidcnlinin c clie . noii subisce ‘climinuzioni sciisibili cli ~iiassn cliirmtc 4a profnsc ; col fissntorc. iiiitocoiidrinlc, oltre a qiicsta sostanza a,,si rcnde eridentc ncl nuclcolo iiiia so- st:inza b, colorabilc con 1’cnintossiliri:i cli Hcidciiliain, sostniizn di cui 11011 si 1i:i trncc.i:i col fiss:itorc ncctibo. La. sost;iiiz:i I ) , coin- penc!tr;itn i111rn11tc I’illtel*f‘iisc coil la. sostnliza ( I , tliiiiinriiscc. sen- sibi’lrncntc: cli qiinntit;i clrrrniitc In profasc cd i: iIllorii :ippunto clic! ri iiiettc a110 scopcrto la sostaiim tij cl10 diimntc l’intcrfasc nor1 nppnrivn ; riconiparc in tclofnsc sotto 1’:ispctto di piccolc iiins-

serclle I I C I ~ f in i c~~o~iiosoriii chinri, cul ~iictoclo Ilclnuiiay, ~ i ins . scrcllc die. condens:iiidosi poi i i i duc iii:issc .priiicipnli, rifoniiano i rliiclcoli iici iiiiclci fig~i. l’rospctt:iro B’ipotcsi ciie l n sostanza 7,

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KICEKCIIE SULL.4 COSTITUZIOiVE DEL I\’UCLEOLO 237

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240 F R;\ IX CI S I

2. - Proro con nltri riiotodi di fissnzioiie.

FI SS.~%IOSI.: RE( a i w . Forrnolo . . . . . . . . . . . . 2 part1 Ricromnto dl potasslo 3% . . . . . S pnrtl.

Duratn ilclln tlssnxlouc 4 gloriil, riiiuov:iiido il llquldo ogni gloriio; iiostcro- inlzzaxione per S glorni in blcromnto di potnsaio 3:;. In corifronto al metotlo Dc- Inunny, ii mctodo 1jcg:iird tlh pcggiorl rlsnltntl, nnchc nel condriomn : i prepnrati EOIIO di climcilc dlffercrizlnzlonc.

Citop1:iuain grigio : condrioin:i cvitlcntc etl ;ildmstn1iz:i nblmn. dnnte ; croriiosouii grigi o grigio-plunibci ; :Issni evidentc il fiiso, clip di profilo I i n I’aslwt to di 1111 c o i p o~iiogciico, pigio. conci- stelite c con contorni iietti. 111 vcdritn polnrc i crornosoiui nitttn- fasici nppniono iiniiiersi in niin iiinssn rischiosn, clic si pro1nng:i attorno n quclli posti cstcrnnnicnte iiclln pinstra, formnndo dclle CUIW fra iiii cromosornn e 4’nltro; :iiiclie in prof:isc i croniosoiiii appnriscono imnicrsi in iinn sostn1iz;i tlcn~n, grigia.

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RZCE’HCIIE SULLA COSTZTUZ/ONE U E L A’UCLEOLO 24 I

171SS.\Zl~lSl~ (’I \LYIfJ.

Forxnolo . . . . . . . . . cc. 4 nlcroninto pot:isslco 6’3; . . . 1) It; Acid0 ncetlco . - . . . . . D 1

Durnta clcllu llssnzlone 2 glorni ; Iiostcronilzznzionc pcr S giornl in bicroninto I1ot:~ssicYJ 3’3;.

Citoplrrsiiin . grigio cliinro ; croniosoiiii iieri : scnwissiiiii gli d o - riiciiti clcl condrionin colornti in iicro : fiiso intlcfinito, rnppiwmi- tnto (la qiinlclic strntturn fil)rill:ir(h.

Xii~lcolo grigio, C\’nll~SCClitc~. chc. lilost ril intcriianicntc IC arcole ; si dcfor11itl IIIdto dtll*:tiitc In I)rofitsc I I I ; ~ 11011 Iliostril nini i\cccIiIio a d lip1 i ci t i l .

11 confront0 fra 1tl fissnzionc cli Ciiiccio c qiiclla di Rcgnid, clic soprnta tto ilifferiscoiio per 1k prcscnzn (1i acid0 acctico nclln priiiin in corifronto nlln sccoiid:i, confcrinx. quniito si era giA trovnto spcrimcntnndo SII nintc~rialc T)ctlniinny, cioia clic I’ncido ncctico Iin un noterole cffctto sul Iiiicldo. pcrclic dopo ~lh siin mime xi011

compnrc piii il costitiicntc 7).

Liq1rido d i C a j a l :

Sltrato d’urniiio . . . . . gr. 1 Formalinn neutrn . . . . . cc. 15 Acqun dlstillntn . . . . . H Si

Liqriido di Da Fano:

Sitrnto dl cobalto . . . . . gr. 1 Formalinn neutrn . . . . . cc. 1.7 AcqUn dlstillntn . . . . . w Ion

Durntn clelln tlsvnzioxic 11 11. 1)opo la tiswzione 110 scguito II inetotla inclirnto c ~ n Cxxcro (IDS) per 13 iipoinncrosi. cioi. 110 ~nscinto i pczzi per pch i rninuti in unn soliizlonc! nl 2% (11 cloriiro cli cmlriiio e poi 11 110 sottopasti n cromizznzlonc In bicroxnnto potnssico nl 3$, :icidlficnto coil ncido ncctico xiclln proporzlone d e l l ’ l ~ , pcr tre glorni n Xo.

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244 F R X S C 1 S I

~0111l1lctn c1infnnizz:izioiie clell’nplcc, coslcciii. smo in renlth gli nlcool piii bassi quell1 rlic fissnno In mngglor-p:irtc dcl1’:iplce. Fissnndo irircce I n nlcool n 9 2 , solo un:i Ptrcttn zonn cli tcasuto posto sull’nssc tlcll’npicc non cllnfnnizzn c qnlndi si Iwrclc prr I’oascrmzlonc aolt:into inin pnrtc clcl plcronin. I rcsultnti dclln flaanaionc in nlcool non sono troppo Inioui, consldcrando l’apicc ncl suo complcsso, mcntrc! FOI~O ribhnstariza 1)noiil iwr un iiiimcro llmltnto di ccllule. Uua roltn mcssi dcntro l’:1c001 i teasnti sl coutrnpgono riolcntcmciitc c In suiierncic dcll’nliicc nlipnre tutt:i pcrcors:i tin grnntli rnglic longltiicllnall. 1.n pnrtc clic iiib riscnte (11 qncstn contr:i- zlonc i! proprio quclln meristcmnticn, clic rirnano scparntq clnl rcsto dclln radice

Oitrc In cuffln, i. lnutilizznbilc Irr I’osscrvazionc qunsi l’intcro clcrmnto:cno, Ic riri ccllulc 1i:inno niiclci clie sl tingono cpniplctnnicntc in iicro coil I’eriintonsilina fcrricx; ncl cluc o t r e strati ccllnlnri ancorn plh intcrni nl clcrnintogcuo Ic cc‘llulc Iiiostrnrio nuclci roii ntltlensniiiciito fortcmcnte croniofilo dclln croiuntinn d n l h pnrtc clell’nssc tlell’nl)lcc; pcrb nlcuni s t ra t i dcl pcriblcmn sono I n condlzlonc rc:l:i- t ivnmriitc buonn c si prcstnuo bcne :ill’osscrnzionc.

dn 1111 FOlCO OriZZOllt~k! J)rOfWldO.

I1 citop1:isiii:i c\ c1iiayissii)io cli toiio c smza contlyionia ; i l i.cticwlo nuclearc e i croiuosoini sono grigio-fcrro: il fuso 6 chiaro di toiio ma evidcntc (!(I in csso si vctlono. qii:intlo corifuwiiicntc qiiando chi:irniiientc, striittiirc f i l ~ i l l i ~ i . I croiiiosotiii in Inetfifiisc WIIO

criclcnternciitc iniiiiorsi in m:i iiinssa COIIIC g:chtinosn. s~bbene di appniwizn pih tenuc’ di qiiando a ~ ~ n f i ~ ~ i i ~ i ~ i l ~ 6 stnta fnttn seguirct la cl*oIi1izziIzionc : talc sostanen intcrcromosoniica si prolungn COIIW

iin tlito di gumto nttoi-no ai cromosomi cstcrni dclln fignix IIW-

tafnsica c si iiiciirvn in :inw profondc fra 1111 croi~iosoiiia. c l’dtro. I1 nuclcolo in intcrfnw 6 iicro e n c o n t o i * n o iiniformc ; tnlroltn

ri i! ncccnno n qunlclie chiiizzn pill cliinra, ilia lion si osscrva nini il tmspnrirc dcllc arcolc. Diirantc In profnsc si cleforma pi*ofo~id:~- inciitc c pcrdc 1111 poco clella sii:~ cromofi1i:i : vcrso ]:I . f i iw t1clI:i. profme, camhiando fnoc0. si vcde spcsso slittaw sotto 1:i 1n:iss:i iicra clcl niiclcolo iiii coi’po piii eIii:ii~o, ~o’l019 grigio-pluiiibco, con ; i r ~ o k t:ilvoltn iiiolto cvitlciiti.

.\lcool nssoluto . ~ . . . 3 parti Acid0 ncctico . . . . . . 1 imtc

Duratn drlln ns:nnzionc 3 11. ; 1):issnggio in nlcool nssoluto c incluslonc.

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KICERCHE SULL.4 COSTITUZlOA’E UEL iVUCLEOZ-0 245

IIo fiss;ito t l i solilo in forinnlo dcl coinnicrclo nl lo$ per 2-4 II., m:i 110 Iiro- r-nto nnclic 1:i. fissnzloiic (11 YAMASOLXIII, seinprc in formolo :i1 lo?;. pcrB con for- xn:ilin:i 1icutr:i ottcnut:i tlistlllnndo qiiclla clcl coiumcrclo in prcscnza di bic:irlio- unto dl sotllo. Tolto il fntto chc col rnctodo Yamanouclil il prc1inr:ito C! vcniito iiiolto ~ic_c,”lo. non vl sono dlffcrcnzc scuslbill fr:i Ic dne fissnzionl.

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216 FRrlS C I S I

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248 F RI\ K C I S I

(1 ) Qwindo ncm vi sinno lndicnzioni sImi(i11, I promxllmcnti delln tccnicn SC-

guitn ncllc tlircrsc prove sono qiielli Intlicnti nel trnttato di Istocliimicn del IA- sos 119:;o.

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RZCERCIIE S D’L LA COS 1‘1 7’ D’Z I ONE D EL A’ U CL E OL 0 249

Efriliio piii teiiui : ci0 conferilia qiinnto osscr’miiiiiio n s i i o tciiipo con la colornzioiic nll’exuntossiliiiu.

DAXGI.:Arin (1937) ili Rliimiiith 11s niajor trovn, con fissnzionc j:cntl:~-3lctvcs, clic i plnstidi si coior:ino i i i rosso coil i l nictodo ~+enlgcii (t iiportn inoltre clic talc coloi*azioiic (lei plnstitli, o piiit- tosto del19aiiii(lo, ern. gih stntn scgri:il:ita cl:i ~.IuI.:I:. Per questo lyl\. riticiic C I I C il illdodo l~culgcil 110n siil spccifico rpcr lln cro- iiintiiin. Egli non dice par6 sc Iin fatto il prclxirnto tli controllo con il solo r w t t i w tli PrIiitF, coriic siirc1iI)c stato iicccssnrio. Aiiclie .

iii l’(z~)/ii~i)cfliZfi))i h’i)iccviu)iuiu lyninitlo si colorii iii r~osso, ma questo rcpcrto 11011 iiifirnin all‘ntto 1:i spccificith, geiier:ilmcntc ani- 111eSPii, tlcl1;i rcnzione cli F C I I ~ ~ ~ I I , clic colisistc in due! tcriipi (1s- sciizinili e iiecessnri : idrolisi :i c:ildo in ricido cloridrico c colorn- zicinc col rcnt t iw d i Sialiiff. I;: infatti l’idrolisi clic rcndc positivo. w i * w il m i t t i v o di ScliiN l’nciclo tiiiioniicleico, die nltrirnenti r i w 1 t a iirgn tivo.

L n rC:izioiic d i I’ciilgcii i: stnta :iilopcr:it:i iiiolto iii qiiesti n’ltiiiii tciiipi per provnrc sc il iiiicleolo contic!nc o no ncido iiiiionuclcico. iii relnxioiic n ~ n . notn tctori:i CIIC fn clel iiiiclcolo 1iii:i rircwn (1i croriintiiin per i croiirosoiiii. La gri1liflc iiinggiornnz:i clellrt prow lin (1:ito risu1t:tto iiegiiivo : il nuc.lcolo nppnrc gcnernliiieritc privo. cli ncitlo tiriioniiclcico. Tiitt:ivin soiio stati ripoi-tnti csciiipi hi cwi 11:~ re:izioric di lkilgeii i. risiiltntn positivn per il iiiiclenlo

Pcr il cnso clie ci ripi:irtI:i, possiamo dire clic clnlln rc:izionc niiclcnlc iion risultn clic riel riiiclcolo cli ~ ~ ~ f ~ l ~ ~ . ~ ~ c ~ ~ i ~ 7 ~ ~ i ~ Spiceria- ~ i / n i si:i p r ~ w i i tc? :ici(lo t iiiioriiiclcico.

(Cfr. ~ A l I A I I A C SUI:lIATSU, 1936 ; (t r)A\KGICAIII)9 1037).

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250 F R.-lXC I S I

3. - Prom per i lipiili.

COI.OR.\ZIOSE COL s~no-Sun.~s B. I1 iirro-Siidnii I! i. iino (lei cn1or:iutf gciicrnll Iwr I Ilpidi. IIO cseguito ~n colornzione con I:I soliiir.lonc s:itiirn :I cnlito 111 a1m01 :i iw.

811 iiintcrlnle fissnto in forinolo lo:(, r scxlonnto nl mlrrotaxiio congc1:itorc. Soi l 110 (Into co1or:izione dl contmsto..

Qncstn rcaziaiic lin dilt!) risiiltato ncg:itivo p r il 1111clco10 c pcr i l iiuclcu>, cleboluicnto positiro pcr il ciihplnsnin. In iiiin scziorw n lircllo clelln prima dif'fcrenzineionc dellc priminc legnose, si notnno fittc grannliiziorii inteimiiientc! positivc siilla Iiicrnlmii? i~cllo strnto piii cstwno dcl dcrn1:itog:ciio cd in qncllo piii interno n cwnt:itto col pcril)lema, stmti clic rnostrnno iIIicllc 11c1 citopl:isni:~. 11113 minione piii intcnsn.

901iostnntc qucsto lprirno i?siilt:ito con un ructodo gciic1;21c~ 110 voliito provarc i riictodi Ciilccio. Infatti SIIIXIX c SIIIGESACA (1X33)

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KICERClIE SULL.,l COSTII‘UZIOIVE U E L II’UCLEOLO 251.

iIo ot tciiiito risiiltnti i i~gi~t ir i :I livcillo d i oyi i cyostitiicJitc! ccl- ill hre.

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c i liliitli : 1111 grnn iiiiiiicro c l l iiicliisl w i s s i Imsnno Iwr t re stntli, lie1 prlniti tlt.1 qiirili diiiino In rc:izioiic tlr4 l i~ i i t l i . ilia no11 I i i rcnzitiiic* plnsiiinlc, ncl sccontlo tlnnno :iinl)cdiir 1c rcnzionl c iirlh sl ntllo finnlc lnfiirc tlnnno 1:i soln rc:izionc ~)lnsinnlc.

I10 nssato il ni:ilerl;ila s in iii cloriiro tli lilntlno 1 ? A , sin nclln iiiisccln in Inr t l ugii;ili tli iiii:i sriliizlonc s:itiir:i cll siib1ini:ito c tli iiitmto dl iir;iiilo. Scl prinio cnso Iio 1mss:ito iiclln soluzlonc snturn tli siililininto lc sczioni ottcniitc col iiilcro- tonio coxigchtore, indi ncl rr:ittivo tli Sc1iiri prr 1.7; r i d sccontlo chso ho iiicz’so

cllrol tniiicntc le sczloni nrl rcnttivo tll Pcliin’; Iio cliiuao w i n gominn-slroppo di Apathy.

* * *

G j h tln liingo tcinlio t cmoscluto 1ic1l:i tcunir;~ niicroaropic:~ I1 fcnonicno tlclla inc!t:ieroni:isin, ciob In pro1irict:i drc! linnno w r t i cnlornnll, cliimicnnicntc tlcfiniti t! nllo stnlo Iniro. tll clnrc 1)ollcrninic. Iwr riii nlcuni c.lcincntl ir;tnlo,“ic.I risiiltnnn coloriti in tinttr n sfnni:itiirc tlivcrsc t h qucllc tlclln soztnnm colornntc stcssn. So1t:into perb cln I>ow tciulio, per rncrlto clcl Lrsos (lo:;), 6 stnto dimostrnto clic 11 vlrappio ~nct:icroni:itico Iin 11 v:ilorc cll iiii:i vcrii c 1irqirl:i rc:ixlonc istoc4iiinic:i. I1 frnoiiierio si Iinsn cw~nzl:ilnicntc sill fa t to clre i colornilti iiict:icronint ici cal- stono sotto tluc foriiic tliverse: iinn Iiorninlc c I’nltm iiirt:icroinnticn. Prcndlnnio ad cs. i1 blau tli toliiithn: 13 sii:i foriiin norinnla & tl’i c*oloru nazurro c In sun foriiin nictiicronintlrn i. tli color(! 1iorlior:i. Scllv soliiaiuiii nccluoac cllluitc ctl in qucllc non :icqi~ose, qunliinqiic sin 1:1 loro cmccntr:izionc. ln fornin st:il)ilc i. In nor1u:ik; ncllc soluaioiii ncquosc concciitrntc U I ~ T ccwn linrtc clcl cnlornntc 1iass;i d:ill:i forrit:i norni:ilc :I clurll:~ niet:icroiiint ictl. cib clic sl tr:itliicc. in iin v1r:iggio dcl colorc tlclln soluzionc tI:ilI*:iaziirrn. nl .violctto. Ti sou0 :ilci~ni fiittorl clic fa- voriscono iiiin fornin rispctto nll’altra : cwsi 1’innnlz:iniento clclln tciii~icr:itiir:i, 1:i prcscnzri tli snli, 1’alb:iusamrnto tlel pII fnvoriscono la fornin norinnlc, niciitrt-, nl contrnrio, 1’:ibbar;s:iinc~nto t1cll:i tcin~icr;itiir:~ c I’ciiinirnto dcl 1111 f:ivorlscono In formn nic!t:irroiiintirn.

U I I ~ i-oltii rc:ilizz:itc IC cont~~zioni in ciii ~n sa1:i forni:i stnbtlc si:i I:I tiorni111c. qunlsinsi sost:inz:i cnlxicc cll f:ir c.oinp:irirc. In fornin niet:icronintirn C niin sostn,t:cr cromolropa. Bost:uizc croniotropc cloi. sono quelic chc slmt:ino l’eqiiilllirio (id sisteinn n tot:ile f:ivore clcllii fornia iuct:icronintlcn. Stiitll istologic1 cd nccii- rnti esgcriiiicnti 111 vitro, riport:iti nell’cxanriaitc c convinccntc iiicinorin tlel LISOS. hnnno tlimostr:itti clic so110 sostaiizc croniotrom csclualmnicntc gli cstcri

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Bibliografin.

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266 F R A S C I S I

Spicgazionc dclln Tavoln XV.

PAPHIOPEDILUII SPICERlANUhl Pfi tz .

F i r i g g i o t . tcl i irrot~inlico dcl bleu di toliiiditia.

Fig. 1 - Intcrfwc in uii:i cvlliiln tlcl perlb1cm:i rnrllcnlc. Hgg. 2, 3, 4 - Stnili siirccsslri tlcllu imofnse. l..ig. 3 - Tnlrio ilclln nictnfasc. Fig. G - J1et:if:isc.

Fig. S - Suclco n ripow. rig. 7 - m.ior:\sc.

Colorcrtiotie col rmno Ri ntfr t i io .

Fig. 9 - OIicrofotogrnfin :I ClillJri c1cll:i sczlonc trnsvcrsn d i UXI :iliicc r:ulic:ilc ncll:i zoii:i clel ~1croiiin-i)criIilciii:i.

XI ASSUSTO. JImlitinte rcnzioni 1stoc.liixiiic.lic sono stntl nicssi i i i c~ltlenzn iicl iiiiclcolo ill

I ’ f i / i l i i o p r [ ~ i ~ i ~ ~ ~ i Spiccriftiitott 1111 cwstitrientc pratclco cd un costitucntc mucillapgi- noso, clic b 1111 cstcrc aolforiw tli iin polisnccnritlu, aostnnza croino!ropn. Wcne csn- nii!intn l’nzlonc. ill rnr l fiss:itivl sui tliic costitucnti dcl nuclcolo, con il rcsu1t:ito 1iriiielp:ilc clic 1 fi,ssntivi ncctlcl tlistruggoxio 11 costitueiitc niucillngfilnoso.

B fitrito Iiic‘sso I n cvicleiizn 11 scgueiitc cIclo clclln niuclllng~iiie nuclcolnrc cro- iiintrolxi : diirnntc In prnfhsc iw: i ~inss:! tlnl iiuclcolo i i c h c:irioliiifn ; tlurnntc I n iiict:if:ise, 1’:iii:if:isc c 1:i tc1of:isc: si ritrov:i iicl fiiso c ncl frnginoplnsto; cl:i rpAln pnrtc clcl fiiso clic rliiiniic C ~ I I I ~ I ~ C P : ~ fr:i i crouiosoini i!el iiiiclci fig11 si ricostriiiscc :ilnicno,. 111 pnrtc niucil1ngginos:i dci lor0 nuclcoll.

Ln sostniim inucil1nginos:i nriclcolnrc si tramandn pcrclb nllc succcsslrc gene- r:izioiii wIlii1:iri uttr;ivcrso I:i for~n:izionc fusorinle: fuso c iillclcolo rappreseiltnno t ~ u c aspetti tlirersi ( I C I I ~ sost:iiim croiiiotrol)n, il priino c1iir:iiitc in niitosi. il sc cwiitlo c1ur:intc l’intcrcincsi. S c l k cclliilc ilcl tcssuto parrnchinintico rndicnle, clie ornicii nnn si tliriiloiio piti. i nuclcoli soiio prii’l d i qucl potcrc cromotropo, clrc nellc cvllulc (lei tcssuti xiicr1stcm:itici rclntiri E loro confcrlto clnlln prcscnzn clel rostitucntc niiicillngginoso tl i Iiiw 11. OS0,II : l’nsscnzn (11 potcrc croniotrolm clnl iiuc*lcolo rnpprcsciitcrc1)bc rliiliitll 1111 lndicc tl i scnilith cclliilnrc (I).

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KUOVO Ciornnle Botnnico Italinno. Nuovn Serie, Vol. XLV, Tnv. XV.

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