EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds...

43
Espressione Genica I: Lo splicing

Transcript of EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds...

Page 1: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

Espressione Genica I: Lo splicing

Page 2: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 3: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 4: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 5: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 6: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

SnRNA U1, U2, U4, U5, U6. Hanno una lunghezza di 100 - 300 nucleotidi.

Gli snRNA si associano a diverse proteine e formano le snRNP (es: snRNP-U1, snRNP-U4)

Spliceosoma (150 proteine + 5 RNA)

Page 7: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 8: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 9: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 10: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 11: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 12: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

Splicing alternativo

Si stima che circa il 60% dei geni umani mostra il fenomeno dello splicing alternativo.

Regolato Diversi messaggeri maturi vengono prodotti da tipi cellulari diversi, oppure in diversi stadi dello sviluppo

Page 13: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 14: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 15: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 16: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 17: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 18: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 19: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 20: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 21: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

RNA Polimerasi eucariotiche

•RNA Pol I 28S, 18S, 5.8S rRNA

•RNA Pol II mRNA; alcuni snRNA

•RNA Pol III tRNA; 5S rRNA; altri snRNA

Page 22: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 23: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 24: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 25: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

RNA Polimerasi eucariotiche

•RNA Pol I 28S, 18S, 5.8S rRNA

•RNA Pol II mRNA; alcuni snRNA

•RNA Pol III tRNA; 5S rRNA; altri snRNA

Page 26: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 27: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 28: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

Espressione Genica II: la Traduzione

Page 29: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 30: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 31: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 32: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 33: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 34: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 35: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called

[XMP]n + XDP = [XMP]n+1 + P

Page 36: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 37: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 38: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 39: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 40: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 41: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 42: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called
Page 43: EspressioneGenicaI: Lo splicingpeople.unica.it/renatorobledo/files/2018/01/Med-09.pdf · SLI binds DNA only in the presence of UBE That factor binds to the upstream half of UCE (called