diagnosticacitogenetica.listarfish.itdiagnosticacitogenetica.listarfish.it/cataloghi/Biologia...

Li StarFish Biologia Cellulare e Molecolare

Catalogo 2010-11

www.listarfish.it 3

Indice generale Citochine, Chemochine, Fattori di Crescita (PeproTech) 5 ELISpot & ELISA Kit, citochine e altri fattori (MabTech) 31 ELISA Kit per lo studio delle implicazioni di ormoni, citochine e altri fattori (BioVendor, RayBiotech, Randox, Jaica, Immundiagnostik) 51 Sistemi magnetici per immunoprecipitazione, separazione cellulare, estrazione acidi nucleici e proteine ricombinanti (Ademtech) 53 Reagenti e kit per transfezione e trasduzione (OZ Biosciences) 67 Reagenti e kit per PCR, RT-PCR e Western Blot (iNtRON Biotechnology) 107 Analisi delle interazioni proteiche (Hypromatrix) 113 Kit di estrazione rapida di acidi nucleici (Omega Bio-Tek) 123


Catalogo 2010-11

[email protected] – [email protected] 4


Catalogo 2010-11

www.listarfish.it 5

PeproTech Citochine, chemochine e fattori di crescita 6

Human 6 Murine 11 Rat 13 Others 14

Anticorpi Monoclonali 15 Anticorpi Policlonali 16

Anti-Human Purificati 16 Anti-Human Biotinilati 19 Anti-Murine Purificati 23 Anti-Murine Biotinilati 24 Anti-Rat Purificati 25 Anti-Rat Biotinilati 26 Control Immunoglobulin 26

ELISA Development Kits (Anticorpi+Standard) 27


Catalogo 2010-11


PeproTech: Citochine, chemochine e fattori di crescita

Human Codice Descrizione Confezione

PEP-310-15 Human 4-1BB (Soluble Receptor) 20 µg

PEP-310-11 Human 4-1BBL 20 µg

PEP-120-14 Human Activin A 5 µg

PEP-120-15 Human Activin B 5 µg

PEP-450-24 Human Adiponectin 25 µg

PEP-130-06 Human ANG-1 20 µg

PEP-310-22 Human AITRL 20 µg

PEP-350-11 Human ApoA1 100 µg

PEP-350-12 Human ApoE2 500 µg



PEP-300-13 Human Apo-SAA 50ug

PEP-300-53 Human Apo-SAA1 50ug

PEP-450-17 Human Artemin 20 µg

PEP-310-13 Human BAFF 20 µg

PEP-310-13R Human BAFF Receptor 50 µg

PEP-300-47 Human BCA-1 (CXCL13) 20 µg

PEP-310-16 Human BCMA 20 µg

PEP-300-51 Human BD-1 (36 a.a.) 20 µg

PEP-300-51A Human BD-1 (47 a.a.) 20 µg

PEP-300-49 Human BD-2 20 µg



PEP-100-50 Human Betacellulin 20 µg

PEP-450-01 Human β-NGF 20 µg

PEP-450-02 Human BDNF 10 µg

PEP-120-04 Human BMP-13/CDMP-2 50 µg

PEP-120-02 Human BMP-2 10 µg

PEP-120-05 Human BMP-4 5 µg

PEP-120-03 Human BMP-7/OP-1 10 µg

PEP-300-50 Human BRAK (CXCL14) 20 µg

PEP-300-32 Human Cardiotrophin-1 10 µg

PEP-100-01 Human CD22 20 µg

PEP-450-13 Human CNTF 20 µg

PEP-300-54 Human CTACK (CCL27) 20 µg

PEP-120-19 Human CTGF (98 a.a.) 20 µg

PEP-120-16 Human CTGFL/WISP-2 20 µg

PEP-300-55 Human CXCL16 25 µg

PEP-120-25 Human CYR61 20 µg

PEP-120-30 Human DKK-1 10 µg

PEP-AF-100-15 Human EGF (Animal Free) 500 µg

PEP-100-44 Human EG-VEGF 20 µg

PEP-100-38 Human EMAP-II 20 µg

PEP-300-22B Human ENA-78 (71 a.a) (CXCL5) 8-78 a.a. 20 µg

PEP-300-22 Human ENA-78 (74 a.a) (CXCL5) 5-78 a.a. 20 µg

PEP-150-01 Human Endostatin 100 µg

PEP-300-33 Human Eotaxin-2 (CCL24) 20 µg

PEP-300-48 Human Eotaxin-3 (CCL26) 20 µg


Catalogo 2010-11

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PeproTech: Citochine, chemochine e fattori di crescita Codice Descrizione Confezione

PEP-100-51 Human Epigen 25 µg

PEP-100-04 Human Epiregulin 25 µg

PEP-300-35 Human Exodus-2 (CCL21) 20 µg

PEP-100-17A Human FGF-acidic 50 µg

PEP-100-18B Human FGF-basic 50 µg

PEP-100-31 Human FGF-4 25 µg













PEP-300-19 Human flt3-Ligand 10 µg

PEP-120-13 Human Follistatin 20 µg

PEP-300-31 Human Fractalkine (CX3CL1) 20 µg

PEP-450-21 Human gAcrp30/Adipolean 25 µg

PEP-450-20 Human gAcrp30/Adipolean Variant 25 µg

PEP-450-39 Human Galectin-1 50 µg

PEP-450-38 Human Galectin-3 50 µg

PEP-300-41 Human GCP-2(CXCL6) 20 µg

PEP-300-23 Human G-CSF 10 µg

PEP-120-11 Human GDF-11 20 µg

PEP-120-28 Human GDF-15/MIC-1 20 µg

PEP-120-22 Human GDF-3 20 µg

PEP-120-23 Human GDF-9 50ug

PEP-450-10 Human GDNF 10 µg

PEP-130-08 Human GLP-1 (7-36°.a.) 1000 µg

PEP-300-03 Human GM-CSF 10 µg

PEP-450-37 Human GMF-β 10 µg

PEP-300-11 Human GRO/MGSA (CXCL1) 25 µg

PEP-300-39 Human GROβ (CXCL2) 10 µg

PEP-300-40 Human GROγ (CXCL3) 10 µg

PEP-100-47 Human HB-EGF 50 µg

PEP-300-38B Human HCC-1 (66 a.a) (CCL14) 10 µg

PEP-300-38 Human HCC-1 (72 a.a) (CCL14) 10 µg

PEP-100-03 Human Heregulinβ-1 50 µg

PEP-100-39 Human HGF 10 µg

PEP-300-27 Human HVEM-Fc 100 µg

PEP-300-37 Human I-309 (CCL1) 10 µg

PEP-150-05 Human ICAM-1 50 µg

PEP-300-02A Human IFN-α 100 µg

PEP-300-02BC Human IFN-β 10 µg

PEP-300-02 Human IFN-γ 100 µg


Catalogo 2010-11



PEP-300-02L Human IFN-λ1 20 µg

PEP-300-02K Human IFN-λ2 20 µg

PEP-350-10 Human IGF-BP1 25 µg

PEP-350-06B Human IGF-BP2 (Insect cell derived) 20 µg


PEP-350-05B Human IGF-BP4 (Insect cell derived) 20 µg



PEP-100-11 Human IGF-I 100 µg

PEP-100-12 Human IGF-II 50 µg

PEP-200-01A Human IL-1α 10 µg

PEP-200-01B Human IL-1β 10 µg

PEP-200-01RA Human IL-1RA 100 µg

PEP-200-02 Human IL-2 50 µg






PEP-200-08M Human IL-8 (72 a.a.) (CXCL8) 25 µg

PEP-200-08 Human IL-8 (77 a.a.) (CXCL8) 25 µg





PEP-200-12p40 Human IL-12p40 10 µg

PEP-200-12p80 Human IL-12p80 10 µg


PEP-200-13A Human IL-13 Variant 10 µg


PEP-200-16A Human IL-16 (121 a.a.) 10 µg

PEP-200-16 Human IL-16 (130 a.a.) 10 µg

PEP-200-17 Human IL-17A (IL-17) 25 µg

PEP-200-28 Human IL-17B 25 µg

PEP-200-27 Human IL-17D 25 µg

PEP-200-24 Human IL-17E 25 µg

PEP-200-25 Human IL-17F 25 µg





PEP-200-33 Human IL-32g 10 µg

PEP-300-12 Human IP-10 (CXCL10) 25 µg

PEP-300-46 Human I-TAC (CXCL11) 20 µg

PEP-100-10 Human KLF4-TAT 25 μg

PEP-100-19 Human KGF/FGF-7 10 µg

PEP-100-53 Human Klotho 20 µg

PEP-300-58 Human LAG-1 (CCL4L1) 20 µg

PEP-300-56 Human LD78β (CCL3L1) 20 µg


Catalogo 2010-11

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PEP-300-44 Human LEC/NCC-4 (CCL16) 20 µg

PEP-300-27 Human Leptin 1000 µg

PEP-310-09B Human Light 15 µg

PEP-300-20 Human Lymphotactin (XCL1) 20 µg

PEP-130-12 Human Maspin 20 µg

PEP-300-04 Human MCP-1/MCAF (CCL2) 20 µg

PEP-300-15 Human MCP-2 (CCL8) 10 µg



PEP-300-25 Human M-CSF 10 µg

PEP-300-36 Human MDC (67 a.a.) (CCL22) 20 µg

PEP-300-36A Human MDC (69 a.a.) (CCL22) 20 µg

PEP-300-57 Human MEC (CCL28) 20 µg

PEP-130-01 Human MIA 20 µg

PEP-130-02 Human MIA-2 20 µg

PEP-450-16 Human Midkine 20 µg

PEP-300-26 Human MIG (CXCL9) 20 µg

PEP-300-08 Human MIP-1α (CCL3) 20 µg

PEP-300-09 Human MIP-1β (CCL4) 10 µg

PEP-300-29 Human MIP-3 (CCL23) 20 µg

PEP-300-29A Human MIP-3α (CCL20) 20 µg

PEP-300-29B Human MIP-3β (CCL19) 20 µg

PEP-300-34 Human MIP-4/PARC (CCL18) 10 µg

PEP-300-43 Human MIP-5 (CCL15) 25 µg

PEP-420-01 Human MMP-1 10 µg

PEP-420-02 Human MMP-2 10 µg

PEP-120-00 Human Myostatin 10 µg

PEP-120-12 Human Myostatin Propeptide 25 µg

PEP-120-21 Human Nanog 20 µg

PEP-300-14 Human NAP-2 (CXCL7) 20 µg

PEP-450-36D Human Neuritin 20 µg

PEP-130-14 Human Neuroserpin 25 µg

PEP-450-11 Human Neurturin 20 µg

PEP-450-18 Human NNT-1/BCSF-3 10 µg

PEP-120-10C Human NOGGIN (cell culture) 20 µg

PEP-120-26 Human NOV 20 µg

PEP-300-42 Human NP-1 20 µg

PEP-450-03 Human NT-3 10 µg

PEP-450-04 Human NT-4 10 µg

PEP-300-10T Human Oncostatin M (209 a.a) 10 µg

PEP-300-10 Human Oncostatin M (227 a.a.) 10 µg

PEP-120-35 Human Osteopontin 50 µg

PEP-450-14 Human Osteoprotegerin (OPG) 50 µg

PEP-130-03 Human OTOR 20 µg

PEP-310-28 Human OX40 ligand 10 µg

PEP-110-02 Human p16-INK4a 20 µg

PEP-140-10 Human PAF-AH 20 µg

PEP-140-04 Human PAI-1 10 µg

PEP-140-06 Human PAI-2 10 µg


Catalogo 2010-11



PEP-100-13A Human PDGF-AA 10 µg

PEP-100-00AB Human PDGF-AB 10 µg

PEP-100-14B Human PDGF-BB 10 µg

PEP-100-00CC Human PDGF-CC 20 µg

PEP-130-13 Human PEDF 20 µg

PEP-450-12 Human Persephin 20 µg

PEP-300-16 Human PF-4 (CXCL4) 20 µg

PEP-450-15 Human Pleiotrophin 20 µg

PEP-100-06 Human PLGF-1 25 µg

PEP-100-56 Human PlGF-2 25 µg

PEP-100-57 Human PlGF-3 25 µg

PEP-100-46 Human Prokineticin-2 20 µg

PEP-100-07 Human Prolactin 50 µg

PEP-100-09 Human PTHrP 50 µg

PEP-300-06 Human RANTES (CCL5) 20 µg

PEP-130-15 Human Relaxin-2 25 µg

PEP-130-10 Human Relaxin-3 25 µg

PEP-450-22 Human RELMβ 25 µg

PEP-450-19 Human Resistin 25 µg

PEP-110-01 Human sCD14 50 µg

PEP-310-26 Human sCD23 20 µg

PEP-310-02 Human sCD40Ligand/TRAP 50 µg

PEP-300-07 Human SCF 10 µg

PEP-100-22A Human SCGF-α 10 µg

PEP-100-22B Human SCGF-β 10 µg

PEP-300-28A Human SDF-1α (CXCL12) 10 µg

PEP-300-28B Human SDF-1β (CXCL12) 10 µg

PEP-140-08 Human sDLL-1 25 µg

PEP-140-07 Human sDLL-4 25 µg

PEP-120-29 Human Secreted Frizzler Related Protein-1 (sFRP-1) 20 µg

PEP-310-03 Human sFas Ligand 10 µg

PEP-310-20 Human sFas Receptor 20 µg

PEP-200-02R Human sIL-2R 25 µg

PEP-200-04R Human sIL-4 Receptor-α 15 µg

PEP-200-06R Human sIL-6 Receptor-α 20 µg

PEP-150-11 Human Slit2-N 25 µg

PEP-100-45 Human Sonic Hedgehog (Shh) (E.coli derived) 25 µg

PEP-100-45B Human Sonic Hedgehog (Shh) (Insect Cell derived) 10 µg

PEP-110-03 Human Sox2 25 µg

PEP-110-03T Human Sox2-TAT 25 µg

PEP-310-08 Human sRANK (Receptor) 100 µg

PEP-310-01 Human sRANKL 10 µg

PEP-310-07 Human sTNF-receptor Type I 20 µg

PEP-310-12 Human sTNF-receptor Type II 20 µg

PEP-310-18 Human sTRAIL Receptor-1 (DR4) 50 µg

PEP-310-19 Human sTRAIL Receptor-2 (DR5) 50 µg

PEP-310-04 Human sTRAIL/Apo2L 50 µg

PEP-310-17 Human TACI 20 µg

PEP-300-63 Human TAFA-2 20 µg


Catalogo 2010-11

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PEP-300-30 Human TARC (CCL17) 20 µg

PEP-300-45 Human TECK (CCL25) 20 µg

PEP-300-59 Human TFF2 20 µg

PEP-100-16A Human TGF-α 100 µg

PEP-100-21C Human TGF-β1 5 µg

PEP-100-21 Human TGF-β1 (mammalian derived) 5 µg

PEP-100-35 Human TGF-β2 5 µg

PEP-100-35B Human TGF-β2 (mammalian derived) 5 µg

PEP-410-01 Human TIMP-1 10 µg

PEP-410-02 Human TIMP-2 10 µg

PEP-310-23 Human TL-1A 20 µg

PEP-160-01 Human TLR-3 25 µg

PEP-300-01A Human TNF-α 50 µg

PEP-300-01B Human TNF-β 20 µg

PEP-300-01BH Human TNF-β (insect cell derived) 20 µg

PEP-300-18 Human TPO 10 µg

PEP-120-19 Human TSG 50 µg

PEP-300-62 Human TSLP 10 µg

PEP-310-06 Human TWEAK 25 µg

PEP-310-21 Human TWEAK Receptor 25 µg

PEP-130-11 Human Vaspin 25 ug

PEP-100-20A Human VEGF121 10 µg

PEP-100-20 Human VEGF165 10 µg

PEP-100-20B Human VEGF-B 20 µg

PEP-100-20C Human VEGF-C 20 µg

PEP-100-20D Human VEGF-D 10 µg

PEP-130-09 Human Visfatin 25 ug

PEP-120-18 Human WISP-1 20 µg

PEP-120-20 Human WISP-3 20 µg

PEP-120-17 Human WNT-1 10 µg

Murine Codice Descrizione Confezione

PEP-315-13 Murine APRIL 20 µg

PEP-250-24 Murine BCA-1/BLC (CXCL13) 20 µg

PEP-450-34 Murine beta-NGF 20 µg

PEP-250-06 Murine C-10 (CCL6) 10 µg

PEP-250-25 Murine Cardiotrophin-1 10 µg

PEP-315-15 Murine CD40 Ligand 25 µg

PEP-250-26 Murine CTACK (CCL27) 20 µg

PEP-250-28 Murine CXCL16 25 µg

PEP-315-09 Murine EGF 500 µg

PEP-250-22 Murine Eotaxin-2 (CCL24) 20 µg

PEP-250-13 Murine Exodus-2 (CCL21) 20 µg

PEP-450-30 Murine FGF-9 10 µg

PEP-450-33A Murine FGF-acidic 50 µg

PEP-450-33 Murine FGF-basic 50 µg

PEP-250-31L Murine flt3-Ligand 10 µg


Catalogo 2010-11



PEP-450-27 Murine gAcrp30 25 µg

PEP-250-05 Murine G-CSF 10 µg

PEP-450-44 Murine GDNF 10 µg

PEP-140-03 Murine Granzyme B 10 µg

PEP-315-03 Murine GM-CSF 10 µg

PEP-315-05 Murine IFN-γ 100 µg

PEP-250-33 Murine IFN-λ2 20 µg

PEP-250-19 Murine IGF-I 50 µg

PEP-211-11A Murine IL-1α 10 µg

PEP-211-11B Murine IL-1β 10 µg

PEP-212-12 Murine IL-2 20 µg









PEP-210-12p80 Murine IL-12p80 10 µg

PEP-210-11 Murine IL-12p40 10 µg



PEP-210-17 Murine IL-17A 25 µg

PEP-210-17F Murine IL-17F 25 µg





PEP-250-16 Murine IP-10 (CXCL10) 25 µg

PEP-250-29 Murine I-TAC (CXCL11) 20 µg

PEP-250-10 Murine JE/MCP-1 (CCL2) 10 µg

PEP-250-11 Murine KC (CXCL1) 20 µg

PEP-450-31 Murine Leptin 1000 µg

PEP-315-12 Murine LIGHT 20 µg

PEP-250-36 Murine LIX/CXCL5 (70 a.a.) 20 µg

PEP-250-17 Murine LIX/CXCL5 (93 a.a.) 20 µg

PEP-250-37 Murine Lungkine 20ug

PEP-250-14 Murine MCP-2 (CCL8) 20 µg



PEP-315-02 Murine M-CSF 10 µg

PEP-250-23 Murine MDC (CCL22) 20 µg

PEP-250-30 Murine MEC (CCL28) 20 µg

PEP-250-18 Murine MIG (CXCL9) 20 µg

PEP-250-09 Murine MIP-1α (CCL3) 10 µg

PEP-250-32 Murine MIP-1β (CCL4) 10 µg

PEP-250-12 Murine MIP-1γ (CCL9/10) 20 µg

PEP-250-15 Murine MIP-2 (CXCL2) 20 µg


Catalogo 2010-11



PEP-250-27 Murine MIP-3α (CCL20) 20 µg

PEP-250-27B Murine MIP-3β (CCL19) 20 µg

PEP-250-25B Murine Neuropoietin 25 µg

PEP-250-38 Murine NOGGIN 20 µg

PEP-315-17 Murine PDGF-AA 10 µg

PEP-315-18 Murine PDGF-BB 10 µg

PEP-450-35 Murine Persephin 20 µg

PEP-250-39 Murine PF-4 (CXCL4) 20 µg

PEP-315-16 Murine Prolactin 50 µg

PEP-250-07 Murine RANTES (CCL5) 20 µg

PEP-450-26 Murine RELMα 25 µg

PEP-450-26B Murine RELMβ 25 µg

PEP-450-26G Murine RELMγ 25 µg

PEP-450-28 Murine Resistin 25 µg

PEP-250-03 Murine SCF 10 µg

PEP-250-20A Murine SDF-1α (CXCL12) 10 µg

PEP-250-20B Murine SDF-1β (CXCL12) 10 µg

PEP-210-25 Murine SF20 10 µg

PEP-315-11 Murine sRANKL 10 µg

PEP-315-01A Murine TNF-α 20 µg

PEP-315-14 Murine TPO 10 µg

PEP-315-19 Murine TRAIL 50 µg

PEP-450-32 Murine VEGF165 10 µg

PEP-315-20 Murine WNT-3a 10 µg

Rat Codice Descrizione Confezione

PEP-450-50 Rat CNTF 25 µg

PEP-400-25 Rat EGF 100 µg

PEP-400-29 Rat FGF-basic 50 µg

PEP-400-26 Rat Fractalkine/CX3CL1 20 µg

PEP-450-51 Rat GDNF 10 µg

PEP-400-23 Rat GM-CSF 10 µg

PEP-400-10 Rat GRO/KC (CXCL1) 25 µg

PEP-400-11 Rat GROβ/MIP-2 (CXCL2) 25 µg

PEP-400-20 Rat IFN-γ 100 µg

PEP-400-01A Rat IL-1α 10 µg

PEP-400-01B Rat IL-1β 10 µg

PEP-400-02 Rat IL-2 20 µg

PEP-400-03 Rat IL-3β 10 µg






PEP-400-19 Rat IL-10 10 µg

PEP-400-16 Rat IL-13 (109aa) 10 µg

PEP-400-16L Rat IL-13 (113aa) 10 µg


Catalogo 2010-11


PeproTech: Citochine, chemochine e fattori di crescita

Rat Codice Descrizione Confezione

PEP-400-24 Rat IL-15 10 µg

PEP-400-33 Rat IP-10 25 µg

PEP-400-21 Rat Leptin 1000 µg

PEP-400-12 Rat MCP-1 (CCL2) 10 µg

PEP-400-28 Rat M-CSF 10 µg

PEP-400-15 Rat MIP-1α (CCL3) 20 µg

PEP-400-09 Rat MIP-1β (CCL4) 20 µg

PEP-400-27 Rat Prolactin 50 µg

PEP-400-13 Rat RANTES (CCL5) 20 µg

PEP-400-35 Rat Resistin 25 µg

PEP-400-22 Rat SCF 10 µg

PEP-400-32A Rat SDF-1 alpha 10 µg

PEP-400-32B Rat SDF-1 beta 10 µg

PEP-400-30 Rat sRANKL 10 µg

PEP-400-14 Rat TNF-α 20 µg

PEP-400-31 Rat VEGF 165 10 µg

Others Codice Descrizione Confezione

PEP-350-03 Viral MIP-2 50 µg


Catalogo 2010-11


PeproTech: Anticorpi Monoclonali

Anticorpi Monoclonali Codice Descrizione Confezione

PEP-500-M64 Anti-Human BAFF 500 μg

PEP-500-M120 Anti-Human BMP-4 500 μg

PEP-500-M123 Anti-Human BMP-7 500 μg

PEP-500-M31 Anti-Human Eotaxin-2 500 μg

PEP-500-M32 Anti-Human Eotaxin-3 500 μg

PEP-500-M40 Anti-Human FGF-5 500 μg

PEP-500-M38 Anti-Human FGF-basic 500 μg

PEP-500-M89 Anti-Human IFN-α 500 μg

PEP-500-M90 Anti-Human IFN-γ 500 μg

PEP-500-M01B Anti-Human IL-1β 500 μg

PEP-500-M02 Anti-Human IL-2 500 μg








PEP-500-M43 Anti-Human LEC 500 μg

PEP-500-M27 Anti-Human Leptin 500 μg

PEP-500-M71 Anti-Human MCP-1 500 μg




PEP-500-M41 Anti-Human MDC 500 μg

PEP-500-M42 Anti-Human MIG 500 μg

PEP-500-M74 Anti-Human MIP-1α 500 μg

PEP-500-M28 Anti-Human MIP-3α 500 μg

PEP-500-M29 Anti-Human MIP-3β 500 μg

PEP-500-M33 Anti-Human NAP-2 500 μg

PEP-500-M24 Anti-Human NT-4 500 μg

PEP-500-M75 Anti-Human RANTES 500 μg

PEP-500-M91 Anti-Human Resistin 500 μg

PEP-500-M44 Anti-Human SCF 500 μg

PEP-500-M02R Anti-Human sIL-2R 500 μg

PEP-500-M46 Anti-Human sRANKL 500 μg

PEP-500-M48 Anti-Human TECK 500 μg

PEP-500-M66 Anti-Human TGFβ1 500 μg

PEP-500-M26 Anti-Human TNF-α 500 μg

PEP-500-M49 Anti-Human TRAIL/ApoII 500 μg

PEP-500-M88 Anti-Human VEGF 500 μg

PEP-500-M85 Anti-Human β-NGF 500 μg


Catalogo 2010-11


PeproTech: Anticorpi Policlonali

Anti-Human Purificati Codice Descrizione Confezione

PEP-500-P167G Anti-Human 4-1BB Receptor 50 µg

PEP-500-P169 Anti-Human 4-1BBL 50 µg

PEP-500-P205 Anti-Human Adipolean Variant 50 µg

PEP-500-P244 Anti-Human AITRL 50 µg

PEP-500-P238 Anti-Human ApoE3 50 µg

PEP-500-P192 Anti-Human APRIL 50 µg

PEP-500-P245 Anti-Human Artemin 50 µg

PEP-500-P163G Anti-Human BAFF 50 µg

PEP-500-P141 Anti-Human BCA-1 50 µg

PEP-500-P253 Anti-Human BD-1 50 µg

PEP-500-P161G Anti-Human BD-2 50 µg


PEP-500-P142G Anti-Human CD40 Ligand 50 µg


PEP-500-P84 Anti-Human BDNF 50 µg

PEP-500-P254 Anti-Human Betacellulin 50 µg

PEP-500-P195 Anti-Human BMP-2 50 µg

PEP-500-P198 Anti-Human BMP-7/OP-1 50 µg

PEP-500-P237 Anti-Human BRAK 50 µg

PEP-500-P101 Anti-Human Cardiotrophin-1 50 µg

PEP-500-P140 Anti-Human CNTF 50 µg

PEP-500-P252 Anti-Human CTGF 50 µg

PEP-500-P212 Anti-Human CTGFL/WISP-2 50 µg

PEP-500-P200 Anti-Human CXCL16 50 µg

PEP-500-P45 Anti-Human EGF 50 µg

PEP-500-P188G Anti-Human EG-VEGF 50 µg

PEP-500-P172G Anti-Human EMAP-II 50 µg

PEP-500-P91 Anti-Human ENA-78 50 µg

PEP-500-P262 Anti-Human Endostatin 50 µg

PEP-500-P103G Anti-Human Eotaxin-2 50 µg

PEP-500-P156G Anti-Human Eotaxin-3 50 µg

PEP-500-P109 Anti-Human Exodus-2 50 µg

PEP-500-P184G Anti-Human FasL/Apo-I 50 µg

PEP-500-P17 Anti-Human FGF-acidic 50 µg

PEP-500-P18 Anti-Human FGF-basic 50 µg

PEP-500-P158 Anti-Human FGF-4 50 µg

PEP-500-P151G Anti-Human FGF-10 50 µg


PEP-500-P152 Anti-Human FGF-17 50 µg


PEP-500-P42 Anti-Human flt3-Ligand 50 µg

PEP-500-P207 Anti-Human Follistatin 50 µg

PEP-500-P98 Anti-Human Fractalkine 50 µg

PEP-500-P288 Anti Human Heregulin beta-1 100 µg

PEP-500-P193G Anti-Human gAcrp30/Adipolean 50 µg

PEP-500-P210 Anti-Human Galectin-1 50 µg

PEP-500-P246 Anti-Human Galectin-3 50 µg

PEP-500-P120 Anti-Human GCP-2 50 µg


Catalogo 2010-11


PeproTech: Anticorpi Policlonali Codice Descrizione Confezione

PEP-500-P43 Anti-Human G-CSF 50 µg

PEP-500-P235 Anti-Human GDF-3 50 µg

PEP-500-P81 Anti-Human GDNF 50 µg

PEP-500-P33 Anti-Human GM-CSF 50 µg

PEP-500-P92 Anti-Human GRO/MGSA 50 µg

PEP-500-P104 Anti-Human GROβ 50 µg

PEP-500-P105 Anti-Human GROγ 50 µg

PEP-500-P106 Anti-Human HCC-1 50 µg

PEP-500-P110 Anti-Human I-309 50 µg

PEP-500-P287 Anti-Human ICAM-1 100 µg

PEP-500-P247 Anti-Human IFN-Lambda-2 50 µg

PEP-500-P32AG Anti-Human IFN-α 50 µg

PEP-500-P32B Anti-Human IFN-β 50 µg

PEP-500-P32 Anti-Human IFN-γ 50 µg

PEP-500-P230 Anti-Human IGF-BP3 50 µg

PEP-500-P232 Anti-Human IGF-BP5 50 µg

PEP-500-P11 Anti-Human IGF-I 50 µg

PEP-500-P12 Anti-Human IGF-II 50 µg

PEP-500-P21A Anti-Human IL-1α 50 µg

PEP-500-P209 Anti-Human IL-1RA 100 µg

PEP-500-P22 Anti-Human IL-2 50 µg

PEP-500-P22G Anti-Human IL-2 50 µg

















PEP-500-P248 Anti-Human IL-17B 50 µg

PEP-500-P89 Anti-Human IL-17E 50 µg

PEP-500-P90 Anti-Human IL-17F 50 µg







PEP-500-P93 Anti-Human IP-10 50 µg

PEP-500-P132 Anti-Human I-TAC 50 µg


Catalogo 2010-11



Codice Descrizione Confezione

PEP-500-P19 Anti-Human KGF 50 µg

PEP-500-P187G Anti-Human LD78β 50 µg

PEP-500-P125G Anti-Human LEC/NCC-4 50 µg

PEP-500-P86 Anti-Human Leptin 50 µg

PEP-500-P179 Anti-Human LIGHT 50 µg

PEP-500-P40 Anti-Human Lymphotactin 50 µg

PEP-500-P34 Anti-Human MCP-1MCAF 50 µg

PEP-500-P35 Anti-Human MCP-2 50 µg

PEP-500-P37G Anti-Human MCP-3 50 µg

PEP-500-P04 Anti-Human MCP-4 50 µg

PEP-500-P04G Anti-Human MCP-4 50 µg

PEP-500-P44 Anti-Human M-CSF 50 µg

PEP-500-P107 Anti-Human MDC 50 µg

PEP-500-P243 Anti-Human MIA 50 µg

PEP-500-P255 Anti-Human MIA-2 50 µg

PEP-500-P171 Anti-Human Midkine 50 µg

PEP-500-P50 Anti-Human MIG 50 µg

PEP-500-P38G Anti-Human MIP-1α 50 µg

PEP-500-P38B Anti-Human MIP-1β 50 µg

PEP-500-P124 Anti-Human MIP-3 50 µg

PEP-500-P95A Anti-Human MIP-3α 50 µg

PEP-500-P95B Anti-Human MIP-3β 50 µg

PEP-500-P108 Anti-Human MIP-4 50 µg

PEP-500-P123G Anti-Human MIP-5 50 µg

PEP-500-P236 Anti-Human Nanog 50 µg

PEP-500-P03 Anti-Human NAP-2 50 µg

PEP-500-P03G Anti-Human NAP-2 50 µg

PEP-500-P271 Anti-Human Neuroserpin 50 µg

PEP-500-P102 Anti-Human Neurturin 50 µg

PEP-500-P186 Anti-Human NNT-1/BCSF-3 50 µg

PEP-500-P257 Anti-Human NOV 50 µg

PEP-500-P126G Anti-Human NP-1 50 µg

PEP-500-P82G Anti-Human NT-3 50 µg

PEP-500-P83G Anti-Human NT-4 50 µg

PEP-500-P30 Anti-Human Oncostatin M 50 µg

PEP-500-P149 Anti-Human OPG 50 µg

PEP-500-P260 Anti-Human PAI-1 50 µg

PEP-500-P226 Anti-Human PIGF 50 µg

PEP-500-P46 Anti-Human PDGF-AA 50 µg

PEP-500-P47 Anti-Human PDGF-BB 50 µg

PEP-500-P138 Anti-Human Persephin 50 µg

PEP-500-P05 Anti-Human PF-4 50 µg

PEP-500-P36 Anti-Human RANTES 50 µg

PEP-500-P217 Anti-Human RELMβ 50 µg

PEP-500-P183G Anti-Human Resistin 50 µg

PEP-500-P227 Anti-Human sCD22 50 µg

PEP-500-P142 Anti-Human sCD40 Ligand 50 µg

PEP-500-P48 Anti-Human SCF 50 µg

PEP-500-P48G Anti-Human SCF 50 µg


Catalogo 2010-11




PEP-500-P99G Anti-Human SCGF-β 50 µg

PEP-500-P87A Anti-Human SDF-1α 50 µg

PEP-500-P87BG Anti-Human SDF-1β 50 µg

PEP-500-P144 Anti-Human sRANK Receptor 50 µg

PEP-500-P133 Anti-Human sRANKL 50 µg

PEP-500-P133G Anti-Human sRANKL 50 µg

PEP-500-P143 Anti-Human sTNF-Receptor Type I 50 µg

PEP-500-P168 Anti-Human sTNF-Receptor Type II 50 µg

PEP-500-P135 Anti-Human TRAIL/Apo2L 50 µg

PEP-500-P166G Anti-Human TACI 50 µg

PEP-500-P280 Anti-Human TIMP-1 50 µg

PEP-500-P97 Anti-Human TARC 50 µg

PEP-500-P134 Anti-Human TECK 50 µg

PEP-500-P16 Anti-Human TGF-α 50 µg

PEP-500-P240 Anti-Human TL-1A 50 µg

PEP-500-P31A Anti-Human TNF-α 50 µg

PEP-500-P31AG Anti-Human TNF-α 50 µg

PEP-500-P31B Anti-Human TNF-β 50 µg

PEP-500-P49G Anti-Human TPO 50 µg

PEP-500-P258 Anti-Human TSLP 50 µg

PEP-500-P137G Anti-Human TWEAK 50 µg

PEP-500-P256 Anti-Human Vaspin 50 µg

PEP-500-P10 Anti-Human VEGF 50 µg

PEP-500-P250 Anti-Human WNT-1 50 µg

PEP-500-P251 Anti-Human WNT-3a 50 µg

PEP-500-P10G Anti-Human VEGF 50 µg

PEP-500-P85 Anti-Human β-NGF 50 µg

Anti-Human Biotinilati Codice Descrizione Confezione

PEP-500-P167Gbt Biotinylated Anti-Human 4-1BB Receptor 25 µg

PEP-500-P169Bt Biotinylated Anti-Human 4-1BBL 25 µg

PEP-500-P193Gbt Biotinylated Anti-Human Adipolean 25 µg

PEP-500-P205bt Biotinylated Anti-Human Adipolean Variant 25 µg

PEP-500-P244bt Biotinylated Anti-Human AITRL 25 µg

PEP-500-P238bt Biotinylated Anti-Human ApoE3 25 µg

PEP-500-P192bt Biotinylated Anti-Human APRIL 25 µg

PEP-500-P245Bt Biotinylated Anti-Human Artemin 25 µg

PEP-500-P163Gbt Biotinylated Anti-Human BAFF 25 µg

PEP-500-P141bt Biotinylated Anti-Human BCA-1 25 µg

PEP-500-P253bt Biotinylated Anti-Human BD-1 25 µg

PEP-500-P161Gbt Biotinylated Anti-Human BD-2 25 µg

PEP-500-P241Bt Biotinylated Anti-Human BD-3 25 µg

PEP-500-P268Bt Biotinylated Anti-Human BD-4 25 µg

PEP-500-P84bt Biotinylated Anti-Human BDNF 25 µg

PEP-500-P254Bt Biotinylated Anti-Human Betacellulin 25 µg

PEP-500-P195bt Biotinylated Anti-Human BMP-2 25 µg


Catalogo 2010-11




PEP-500-P198bt Biotinylated Anti-Human BMP-7/OP-1 25 µg

PEP-500-P237Bt Biotinylated Anti-Human BRAK 25 µg

PEP-500-P101bt Biotinylated Anti-Human Cardiotrophin-1 25 µg

PEP-500-P140bt Biotinylated Anti-Human CNTF 25 µg

PEP-500-P252Bt Biotinylated Anti-Human CTGF 25 µg

PEP-500-P212bt Biotinylated Anti-Human CTGFL/WISP-2 25 µg

PEP-500-P200bt Biotinylated Anti-Human CXCL16 25 µg

PEP-500-P45bt Biotinylated Anti-Human EGF 25 µg

PEP-500-P188Gbt Biotinylated Anti-Human EG-VEGF 25 µg

PEP-500-P172Gbt Biotinylated Anti-Human EMAP-II 25 µg

PEP-500-P91bt Biotinylated Anti-Human ENA-78 25 µg

PEP-500-P103Gbt Biotinylated Anti-Human Eotaxin-2 25 µg

PEP-500-P156Gbt Biotinylated Anti-Human Eotaxin-3 25 µg

PEP-500-P262Bt Biotinylated Anti-Human Endostatin 25 µg

PEP-500-P109bt Biotinylated Anti-Human Exodus-2 25 µg

PEP-500-P184Gbt Biotinylated Anti-Human FasL/Apo-I 25 µg

PEP-500-P17bt Biotinylated Anti-Human FGF-acidic 25 µg

PEP-500-P18bt Biotinylated Anti-Human FGF-basic 25 µg

PEP-500-P158bt Biotinylated Anti-Human FGF-4 25 µg

PEP-500-P151Gbt Biotinylated Anti-Human FGF-10 25 µg


PEP-500-P152bt Biotinylated Anti-Human FGF-17 25 µg


PEP-500-P42bt Biotinylated Anti-Human flt3-Ligand 25 µg

PEP-500-P207Bt Biotinylated Anti-Human Follistatin 25 µg

PEP-500-P98bt Biotinylated Anti-Human Fractalkine 25 µg

PEP-500-P210bt Biotinylated Anti-Human Galectin-1 25 µg

PEP-500-P246bt Biotinylated Anti-Human Galectin-3 25 µg

PEP-500-P120bt Biotinylated Anti-Human GCP-2 25 µg

PEP-500-P43bt Biotinylated Anti-Human G-CSF 25 µg

PEP-500-P235bt Biotinylated Anti-Human GDF-3 25 µg

PEP-500-P81bt Biotinylated Anti-Human GDNF 25 µg

PEP-500-P33bt Biotinylated Anti-Human GM-CSF 25 µg

PEP-500-P92bt Biotinylated Anti-Human GRO/MGSA 25 µg

PEP-500-P104bt Biotinylated Anti-Human GROβ 25 µg

PEP-500-P105bt Biotinylated Anti-Human GROγ 25 µg

PEP-500-P106bt Biotinylated Anti-Human HCC-1 25 µg

PEP-500-P288Bt Biotinylated Anti-Human Heregulin beta-1 50 µg

PEP-500-P110bt Biotinylated Anti-Human I-309 25 µg

PEP-500-P287Bt Biotinylated Anti-Human ICAM-1 50 µg

PEP-500-P247bt Biotinylated Anti-Human IFN-Lambda-2 25 µg

PEP-500-P32Abt Biotinylated Anti-Human IFN-α 25 µg

PEP-500-P32Bbt Biotinylated Anti-Human IFN-β 25 µg

PEP-500-P32bt Biotinylated Anti-Human IFN-γ 25 µg

PEP-500-P230bt Biotinylated Anti-Human IGF-BP3 25 µg



PEP-500-P11bt Biotinylated Anti-Human IGF-I 25 µg

PEP-500-P12bt Biotinylated Anti-Human IGF-II 25 µg


Catalogo 2010-11



PEP-500-P20bt Biotinylated Anti-Human IL-10 25 µg

PEP-500-P21Abt Biotinylated Anti-Human IL-1α 25 µg

PEP-500-P209Bt Biotinylated Anti-Human IL-1RA 50 µg


PEP-500-P22Gbt Biotinylated Anti-Human IL-2 25 µg















PEP-500-P07bt Biotinylated Anti-Human IL-17A 25 µg

PEP-500-P248Bt Biotinylated Anti-Human IL-17B 25 µg

PEP-500-P89bt Biotinylated Anti-Human IL-17E 25 µg

PEP-500-P90bt Biotinylated Anti-Human IL-17F 25 µg




PEP-500-P191Bt Biotinylated Anti-Human IL-21 25 µg


PEP-500-P261Bt Biotinylated Anti-Human IL-33 25 µg

PEP-500-P93bt Biotinylated Anti-Human IP-10 25 µg

PEP-500-P132bt Biotinylated Anti-Human I-TAC 25 µg

PEP-500-P19bt Biotinylated Anti-Human KGF 25 µg

PEP-500-P125bt Biotinylated Anti-Human LEC 25 µg

PEP-500-P187Gbt Biotinylated Anti-Human LD78β 25 µg

PEP-500-P125Gbt Biotinylated Anti-Human LEC/NCC-4 25 µg

PEP-500-P86bt Biotinylated Anti-Human Leptin 25 µg

PEP-500-P179bt Biotinylated Anti-Human LIGHT 25 µg

PEP-500-P40bt Biotinylated Anti-Human Lymphotactin 25 µg

PEP-500-P34bt Biotinylated Anti-Human MCP-1MCAF 25 µg

PEP-500-P35bt Biotinylated Anti-Human MCP-2 25 µg

PEP-500-P37Gbt Biotinylated Anti-Human MCP-3 25 µg

PEP-500-P04bt Biotinylated Anti-Human MCP-4 25 µg

PEP-500-P04Gbt Biotinylated Anti-Human MCP-4 25 µg

PEP-500-P44bt Biotinylated Anti-Human M-CSF 25 µg

PEP-500-P107bt Biotinylated Anti-Human MDC 25 µg

PEP-500-P243bt Biotinylated Anti-Human MIA 25 µg

PEP-500-P255bt Biotinylated Anti-Human MIA-2 25 µg

PEP-500-P171bt Biotinylated Anti-Human Midkine 25 µg

PEP-500-P50bt Biotinylated Anti-Human MIG 25 µg


Catalogo 2010-11



PEP-500-P38Gbt Biotinylated Anti-Human MIP-1α 25 µg

PEP-500-P38BGbt Biotinylated Anti-Human MIP-1β 25 µg

PEP-500-P38Bbt Biotinylated Anti-Human MIP-1β 25 µg

PEP-500-P124bt Biotinylated Anti-Human MIP-3 25 µg

PEP-500-P95Abt Biotinylated Anti-Human MIP-3α 25 µg

PEP-500-P95Bbt Biotinylated Anti-Human MIP-3β 25 µg

PEP-500-P108bt Biotinylated Anti-Human MIP-4 25 µg

PEP-500-P123Gbt Biotinylated Anti-Human MIP-5 25 µg

PEP-500-P236bt Biotinylated Anti-Human Nanog 25 µg

PEP-500-P03bt Biotinylated Anti-Human NAP-2 25 µg

PEP-500-P3Gbt Biotinylated Anti-Human NAP-2 25 µg

PEP-500-P186Bt Biotinylated Anti-Human NNT-1/BCSF-3 25 µg

PEP-500-P126Gbt Biotinylated Anti-Human NP-1 25 µg

PEP-500-P82Gbt Biotinylated Anti-Human NT-3 25 µg

PEP-500-P83bt Biotinylated Anti-Human NT-4 25 µg

PEP-500-P83Gbt Biotinylated Anti-Human NT-4 25 µg

PEP-500-P271Bt Biotinylated Anti-Human Neuroserpin 25 µg

PEP-500-P102Bt Biotinylated Anti-Human Neurturin 25 µg

PEP-500-P257bt Biotinylated Anti-Human NOV 25 µg

PEP-500-P30bt Biotinylated Anti-Human Oncostatin M 25 µg

PEP-500-P149bt Biotinylated Anti-Human OPG 25 µg

PEP-500-P260bt Biotinylated Anti-Human PAI-1 25 µg

PEP-500-P46bt Biotinylated Anti-Human PDGF-AA 25 µg

PEP-500-P47bt Biotinylated Anti-Human PDGF-BB 25 µg

PEP-500-P226bt Biotinylated Anti-Human PIGF 25 µg

PEP-500-P138Bt Biotinylated Anti-Human Persephin 25 µg

PEP-500-P05bt Biotinylated Anti-Human PF-4 25 µg

PEP-500-P36bt Biotinylated Anti-Human RANTES 25 µg

PEP-500-P217bt Biotinylated Anti-Human RELMβ 25 µg

PEP-500-P183Gbt Biotinylated Anti-Human Resistin 25 µg

PEP-500-P227bt Biotinylated Anti-Human CD22 25 µg

PEP-500-P142bt Biotinylated Anti-Human sCD40 Ligand 25 µg

PEP-500-P142Gbt Biotinylated Anti-Human sCD40 Ligand 25 µg

PEP-500-P48bt Biotinylated Anti-Human SCF 25 µg

PEP-500-P48Gbt Biotinylated Anti-Human SCF 25 µg

PEP-500-P99Gbt Biotinylated Anti-Human SCGF-β 25 µg

PEP-500-P87Abt Biotinylated Anti-Human SDF-1α 25 µg

PEP-500-P87BGbt Biotinylated Anti-Human SDF-1β 25 µg

PEP-500-P144bt Biotinylated Anti-Human sRANK Receptor 25 µg

PEP-500-P133bt Biotinylated Anti-Human sRANKL 25 µg

PEP-500-P133Gbt Biotinylated Anti-Human sRANKL 25 µg

PEP-500-P143bt Biotinylated Anti-Human sTNF-Receptor Type I 25 µg

PEP-500-P168bt Biotinylated Anti-Human sTNF-Receptor Type II 25 µg

PEP-500-P135bt Biotinylated Anti-Human sTRAIL/Apo2L 25 µg

PEP-500-P166Gbt Biotinylated Anti-Human TACI 25 µg

PEP-500-P97bt Biotinylated Anti-Human TARC 25 µg

PEP-500-P134bt Biotinylated Anti-Human TECK 25 µg

PEP-500-P16bt Biotinylated Anti-Human TGF-α 25 µg

PEP-500-P280Bt Biotinylated Anti-Human TIMP-1 25 µg


Catalogo 2010-11



PEP-500-P240bt Biotinylated Anti-Human TL-1A 25 µg

PEP-500-P31Abt Biotinylated Anti-Human TNF-α 25 µg

PEP-500-P31Agbt Biotinylated Anti-Human TNF-α 25 µg

PEP-500-P31Bbt Biotinylated Anti-Human TNF-β 25 µg

PEP-500-P49Gbt Biotinylated Anti-Human TPO 25 µg

PEP-500-P258bt Biotinylated Anti-Human TSLP 25 µg

PEP-500-P137Gbt Biotinylated Anti-Human TWEAK 25 µg

PEP-500-P256bt Biotinylated Anti-Human Vaspin 25 µg

PEP-500-P10bt Biotinylated Anti-Human VEGF 25 µg

PEP-500-P10Gbt Biotinylated Anti-Human VEGF 25 µg

PEP-500-P250bt Biotinylated Anti-Human WNT-1 25 µg

PEP-500-P251Bt Biotinylated Anti-Human WNT-3a 25 µg

PEP-500-P85bt Biotinylated Anti-Human β-NGF 25 µg

Anti-Murine Purificati Codice Descrizione Confezione

PEP-500-P128 Anti-Murine B71 50 µg

PEP-500-P112 Anti-Murine C-10 50 µg

PEP-500-P147 Anti-Murine CTLA-4 50 µg

PEP-500-P201G Anti-Murine CXCL16 50 µg

PEP-500-P174G Anti-Murine EGF 50 µg

PEP-500-P175G Anti-Murine Eotaxin-2 50 µg

PEP-500-P114 Anti-Murine Exodus-2 50 µg

PEP-500-P66 Anti-Murine FGF-9 50 µg

PEP-500-P69 Anti-Murine G-CSF 50 µg

PEP-500-P65 Anti-Murine GM-CSF 50 µg

PEP-500-P119 Anti-Murine IFN-γ 50 µg

PEP-500-P157G Anti-Murine IGF-I 50 µg

PEP-500-P51A Anti-Murine IL-1α 50 µg

PEP-500-P51 Anti-Murine IL-1β 50 µg

PEP-500-P111 Anti-Murine IL-2 50 µg







PEP-500-M128 Anti-Murine IL-10 500 µg

PEP-500-P155G Anti-Murine IL-12 50 µg

PEP-500-M59 Anti-Murine IL-12 500 μg



PEP-500-P265 Anti-Murine IL-17A 50 µg

PEP-500-P153G Anti-Murine IL-18BP 50 µg



PEP-500-P129 Anti-Murine IP-10 50 µg


Catalogo 2010-11



PEP-500-P113 Anti-Murine JE (MCP-1) 50 µg

PEP-500-P115 Anti-Murine KC 50 µg

PEP-500-P68 Anti-Murine Leptin 50 µg

PEP-500-P146 Anti-Murine LIX 50 µg

PEP-500-P127 Anti-Murine MCP-2 50 µg

PEP-500-P116G Anti-Murine MCP-3 50 µg

PEP-500-P61 Anti-Murine MCP-5 50 µg

PEP-500-P62G Anti-Murine M-CSF 50 µg

PEP-500-P176 Anti-Murine MDC 50 µg

PEP-500-P121 Anti-Murine MIP-1α 50 µg

PEP-500-P213 Anti-Murine MIP-1β 50 µg

PEP-500-P117 Anti-Murine MIP-1γ 50 µg

PEP-500-P130 Anti-Murine MIP-2 50 µg

PEP-500-P118 Anti-Murine RANTES 50 µg

PEP-500-P214 Anti-Murine RELMα 50 µg

PEP-500-P215 Anti-Murine RELMβ 50 µg

PEP-500-P182G Anti-Murine Resistin 50 µg

PEP-500-P71 Anti-Murine SCF 50 µg

PEP-500-P164G Anti-Murine SDF-1α 50 µg

PEP-500-P259 Anti-Murine SF-20 50 µg

PEP-500-P63 Anti-Murine sRANKL 50 µg

PEP-500-P64 Anti-Murine TNF-α 50 µg

PEP-500-P64G Anti-Murine TNF-α 50 µg

PEP-500-P131 Anti-Murine VEGF 50 µg

Anti-Murine Biotinilati Codice Descrizione Confezione

PEP-500-P128bt Biotinylated Anti-Murine B71 25 µg

PEP-500-P112bt Biotinylated Anti-Murine C-10 25 µg

PEP-500-P147bt Biotinylated Anti-Murine CTLA-4 25 µg

PEP-500-P201Gbt Biotinylated Anti-Murine CXCL16 25 µg

PEP-500-P174Gbt Biotinylated Anti-Murine EGF 25 µg

PEP-500-P175Gbt Biotinylated Anti-Murine Eotaxin-2 25 µg

PEP-500-P114bt Biotinylated Anti-Murine Exodus-2 25 µg

PEP-500-P66bt Biotinylated Anti-Murine FGF-9 25 µg

PEP-500-P69bt Biotinylated Anti-Murine G-CSF 25 µg

PEP-500-P65bt Biotinylated Anti-Murine GM-CSF 25 µg

PEP-500-P119bt Biotinylated Anti-Murine IFN-γ 25 µg

PEP-500-P157Gbt Biotinylated Anti-Murine IGF-I 25 µg

PEP-500-P51Abt Biotinylated Anti-Murine IL-1α 25 µg

PEP-500-P51bt Biotinylated Anti-Murine IL-1β 25 µg

PEP-500-P111bt Biotinylated Anti-Murine IL-2 25 µg








Catalogo 2010-11



PEP-500-P155Gbt Biotinylated Anti-Murine IL-12 25 µg


PEP-500-P173Bt Biotinylated Anti-Murine IL-15 25 µg

PEP-500-P265Bt Biotinylated Anti-Murine IL-17A 25 µg

PEP-500-P153Gbt Biotinylated Anti-Murine IL-18BP 25 µg



PEP-500-P129bt Biotinylated Anti-Murine IP-10 25 µg

PEP-500-P113bt Biotinylated Anti-Murine JE (MCP-1) 25 µg

PEP-500-P115bt Biotinylated Anti-Murine KC 25 µg

PEP-500-P68bt Biotinylated Anti-Murine Leptin 25 µg

PEP-500-P146bt Biotinylated Anti-Murine LIX 25 µg

PEP-500-P127bt Biotinylated Anti-Murine MCP-2 25 µg

PEP-500-P116Gbt Biotinylated Anti-Murine MCP-3 25 µg

PEP-500-P61bt Biotinylated Anti-Murine MCP-5 25 µg

PEP-500-P62Gbt Biotinylated Anti-Murine M-CSF 25 µg

PEP-500-P176bt Biotinylated Anti-Murine MDC 25 µg

PEP-500-P121bt Biotinylated Anti-Murine MIP-1α 25 µg

PEP-500-P213bt Biotinylated Anti-Murine MIP-1β 25 µg

PEP-500-P117bt Biotinylated Anti-Murine MIP-1γ 25 µg

PEP-500-P130bt Biotinylated Anti-Murine MIP-2 25 µg

PEP-500-P118bt Biotinylated Anti-Murine RANTES 25 µg

PEP-500-P214Bt Biotinylated Anti-Murine RELMα 25 µg

PEP-500-P215bt Biotinylated Anti-Murine RELMβ 25 µg

PEP-500-P182Gbt Biotinylated Anti-Murine Resistin 25 µg

PEP-500-P71bt Biotinylated Anti-Murine SCF 25 µg

PEP-500-P259bt Biotinylated Anti-Murine SCF 25 µg

PEP-500-P164Gbt Biotinylated Anti-Murine SDF-1α 25 µg

PEP-500-P63bt Biotinylated Anti-Murine sRANKL 25 µg

PEP-500-P64bt Biotinylated Anti-Murine TNF-α 25 µg

PEP-500-P64Gbt Biotinylated Anti-Murine TNF-α 25 µg

PEP-500-P131bt Biotinylated Anti-Murine VEGF 25 µg

Anti-Rat Purificati Codice Descrizione Confezione

PEP-500-P79 Anti-Rat CNTF 50 µg

PEP-500-P277 Anti-Rat EGF 50 µg

PEP-500-P74 Anti-Rat GRO/KC 50 µg

PEP-500-P75 Anti-Rat GROβ/MIP-2 50 µg

PEP-500-P180G Anti-Rat IL-1α 50 µg

PEP-500-P80 Anti-Rat IL-1β 50 µg

PEP-500-P274 Anti-Rat IL-2 50 µg

PEP-500-P177G Anti-Rat IL-3β 50 µg



PEP-500-P73G Anti-Rat IL-6 50 µg


PEP-500-P122G Anti-Rat IFN-γ 50 µg


Catalogo 2010-11



PEP-500-P290 Anti-Rat IP-10 100 µg

PEP-500-P185G Anti-Rat Leptin 50 µg

PEP-500-P76 Anti-Rat MCP-1MCAF 50 µg

PEP-500-P77 Anti-Rat MIP-1α 50 µg

PEP-500-P78 Anti-Rat RANTES 50 µg

PEP-500-P202G Anti-Rat SCF 50 µg

PEP-500-P72 Anti-Rat TNF-α 50 µg

PEP-500-P275 Anti-Rat VEGF 50 µg

Anti-Rat Biotinilati Codice Descrizione Confezione

PEP-500-P79bt Biotinylated Anti-Rat CNTF 25 µg

PEP-500-P74bt Biotinylated Anti-Rat GRO/KC 25 µg

PEP-500-P75bt Biotinylated Anti-Rat GROβ/MIP-2 25 µg

PEP-500-P277bt Biotinylated Anti-Rat EGF 25 µg

PEP-500-P122bt Biotinylated Anti-Rat IFN-γ 25 µg

PEP-500-P122Gbt Biotinylated Anti-Rat IFN-γ 25 µg

PEP-500-P180Gbt Biotinylated Anti-Rat IL-1α 25 µg

PEP-500-P80bt Biotinylated Anti-Rat IL-1β 25 µg

PEP-500-P274bt Biotinylated Anti-Rat IL-2 25 µg

PEP-500-P177Gbt Biotinylated Anti-Rat IL-3β 25 µg



PEP-500-P73Gbt Biotinylated Anti-Rat IL-6 25 µg


PEP-500-P290Bt Biotinylated Anti-Rat IP-10 50 µg

PEP-500-P185Gbt Biotinylated Anti-Rat Leptin 25 µg

PEP-500-P76bt Biotinylated Anti-Rat MCP-1MCAF 25 µg

PEP-500-P77bt Biotinylated Anti-Rat MIP-1α 25 µg

PEP-500-P78bt Biotinylated Anti-Rat RANTES 25 µg

PEP-500-P202Gbt Biotinylated Anti-Rat SCF 25 µg

PEP-500-P72bt Biotinylated Anti-Rat TNF-α 25 µg

PEP-500-P275bt Biotinylated Anti-Rat VEGF 25 µg

Control Immunoglobulin Codice Descrizione Confezione

PEP-500-G00 Control Goat Immunoglobin 1 mg

PEP-500-M00 Control Mouse Immunoglobulin 250 μg

PEP-500-P00 Control Rabbit Immunoglobin 500 µg


Catalogo 2010-11


PeproTech: ELISA Development Kits

ELISA Development Kits (Anticorpi+Standard+HRP-Avidin) Codice Descrizione Confezione

PEP-900-K208 Human 4-1BB Receptor 10 plates

PEP-900-K202 Human BD-1 10 plates

PEP-900-M202 Human BD-1 Mini 2 plates



PEP-900-K372 Human Betacellulin 10 plates

PEP-900-K255 Human BMP-2 10 plates

PEP-900-K158 Human CNTF 10 plates

PEP-900-K317 Human CTGF 10 plates

PEP-900-K230 Human CXCL16 10 plates

PEP-900-M230 Human CXCL16 Mini 2 plates

PEP-900-K05 Human EGF 10 plates

PEP-900-K244 Human EG-VEGF 10 plates

PEP-900-K167 Human Eotaxin-3 10 plates

PEP-900-K08 Human FGF-basic 10 plates

PEP-900-M08 Human FGF-basic Mini 2 plates

PEP-900-K299 Human Follistatin 10 plates

PEP-900-K77 Human G-CSF 10 plates

PEP-900-K30 Human GM-CSF 10 plates

PEP-900-K38 Human GRO α/MGSA 10 plates

PEP-900-K120 Human GRO β 10 plates

PEP-900-K27 Human IFN-γ 10 plates

PEP-900-M27 Human IFN-γ Mini 2 plates

PEP-900-K11 Human IL-α 10 plates

PEP-900-K12 Human IL-2 10 plates












PEP-900-K84 Human IL-17/IL-17A 10 plates

PEP-900-M84 Human IL-17A Mini 2 plates

PEP-900-K234 Human IL-17E 10 plates

PEP-900-M234 Human IL-17E Mini 2 plates

PEP-900-K277 Human IL-17F 10 plates

PEP-900-M12 Human IL-2 Mini 2 plates








Catalogo 2010-11


PeproTech: ELISA Development Kits Codice Descrizione Confezione



PEP-900-K39 Human IP-10 10 plates

PEP-900-K151 Human I-TAC 10 plates

PEP-900-K31 Human MCP-1 10 plates

PEP-900-K357 Human MIA-2 10 plates

PEP-900-K190 Human Midkine 10 plates

PEP-900-K35 Human MIP-1α 10 plates

PEP-900-K40 Human NAP-2 10 plates

PEP-900-K338 Human NOV 10 plates

PEP-900-K307 Human P1GF-1 10 plates

PEP-900-K383 Human PAI 10 plates

PEP-900-K04 Human PDGF-BB 10 plates

PEP-900-K33 Human RANTES 10 plates

PEP-900-M33 Human RANTES Mini 2 plates

PEP-900-K235 Human Resistin 10 plates

PEP-900-K145 Human sCD40L 10 plates

PEP-900-M145 Human sCD40 Mini 2 plates

PEP-900-K34 Human SCF 10 plates

PEP-900-K142 Human sRANKL 10 plates

PEP-900-K141 Human Strail/APO2L 10 plates

PEP-900-K216 Human TACI 10 plates

PEP-900-K290 Human TL-1A 10 plates

PEP-900-K25 Human TNF-α 10 plates

PEP-900-M25 Human TNF-α Mini 2 plates

PEP-900-K44 Human TPO 10 plates

PEP-900-K334 Human TSLP 10 plates

PEP-900-K149 Human TWEAK 10 plates

PEP-900-K10 Human VEGF 10 plates

PEP-900-M10 Human VEGF Mini 2 plates

PEP-900-K60 Human β-NGF 10 plates

PEP-900-K179 Murine EGF 10 plates

PEP-900-K103 Murine G-CSF 10 plates

PEP-900-K55 Murine GM-CSF 10 plates

PEP-900-K98 Murine IFN-γ 10 plates

PEP-900-M98 Murine IFN-γ Mini 2 plates

PEP-900-K170 Murine IGF-1 10 plates

PEP-900-K82 Murine IL-1α 10 plates

PEP-900-K47 Murine IL-1β 10 plates

PEP-900-M47 Murine IL-1β Mini 2 plates

PEP-900-K108 Murine IL-2 10 plates






PEP-900-M97 Murine IL-12 Mini 2 plates


PEP-900-M207 Murine IL-13 Mini 2 plates


Catalogo 2010-11


PeproTech: ELISA Development Kits Codice Descrizione Confezione

PEP-900-M153 Murine IP-10 Mini 2 plates

PEP-900-K126 Murine JE/MPC1 10 plates

PEP-900-M126 Murine JE/MPC1 Mini 2 plates

PEP-900-K127 Murine KC 10 plates

PEP-900-M127 Murine KC Mini 2 plates

PEP-900-K76 Murine Leptin 10 plates

PEP-900-K123 Murine MCP-3 10 plates

PEP-900-K245 Murine M-CSF 10 plates

PEP-900-K197 Murine MDC 10 plates

PEP-900-K125 Murine MIP-1α 10 plates

PEP-900-K278 Murine MIP-1β 10 plates

PEP-900-M278 Murine MIP-1β Mini 2 plates

PEP-900-K152 Murine MIP-2 10 plates

PEP-900-K124 Murine RANTES 10 plates

PEP-900-K78 Murine SCF 10 plates

PEP-900-K233 Murine sRANKL 10 plates

PEP-900-K54 Murine TNF-α 10 plates

PEP-900-M54 Murine TNF-α Mini 2 plates

PEP-900-K99 Murine VEGF 10 plates

PEP-900-K57 Rat GRO-KC 10 plates

PEP-900-K109 Rat IFN-γ 10 plates

PEP-900-K204 Rat IL-1α 10 plates

PEP-900-K91 Rat IL-β 10 plates

PEP-900-K86 Rat IL-6 10 plates

PEP-900-M86 Rat IL-6 Mini 2 plates

PEP-900-K59 Rat MCP-1 10 plates

PEP-900-K75 Rat MIP-1 α 10 plates

PEP-900-K72 Rat RANTES 10 plates

PEP-900-K258 Rat SCF 10 plates

PEP-900-K73 Rat TNF-α 10 plates


Catalogo 2010-11



Catalogo 2010-11


MabTech Caratteristiche dei kit per ELISpot ed ELISA 32 ELISpot & ELISA Kits 33

Human 33 Monkey 40 Mouse 42 Bovine 45 Ovine 45 Equine 45 Porcine 46 Rat 46

Altri (Streptavidin, Anti-CD, etc) 47


Catalogo 2010-11


MabTech: caratteristiche dei kit per ELISpot ed ELISA • ELISpot PRO Kit: precoated PVDF plates, ALP-labelled detection mAb,

BCIP/NBT substrate • ELISpot PLUS Kit: PVDF plates, coating mAb, biotinylated detection mAb,

Streptavidin-ALP, BCIP/NBT substrate • ELISpot Kit: coating mAb, biotinylated detection mAb, Streptavidin-enzyme

(ALP or HRP) • ELISA Kit: coating mAb, biotinylated detection mAb, Streptavidin-enzyme (ALP

or HRP), cytokine standard • ApoA1 ELISA kit: coating mAb, biotinylated detection mAb, Streptavidin-

enzyme (ALP or HRP) • Galectin-3 HRP kit: HRP-labelled detection mAb, blocking reagent Le piastre sono disponibili in formato trasparente o bianco. La maggior parte dei lettori (Zeiss, AID, Biosys) può utilizzarle entrambe; in questo caso Vi suggeriamo di operare con piastre trasparenti. Altri lettori (CTL, Aelvis) danno migliori risultati con le piastre bianche.


Catalogo 2010-11


MabTech: Human ELISpot & ELISA Kits

Human Interferon-gamma Codice Descrizione Confezione MAB-3420-1A-6 Human IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3420-1A-20 Human IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3420-1H-6 Human IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3420-1H-20 Human IFN-g ELISA (HRP) (for 20 plates) 1 kit

MAB-3420-1HP-2 Human IFN-g ELISA PRO Kit (for 2 plates) 1 kit

MAB-3420-1HP-10 Human IFN-g ELISA PRO Kit (for 10 plates) 1 kit

MAB-3420-2A Human IFN-g ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3420-2APT-2 Human IFN-g ELISpot PRO Kit transparent (ALP) (for 2 plates) 1 kit

MAB-3420-2APT-10 Human IFN-g ELISpot PRO Kit transparent (ALP) (for 10 plates) 1 kit

MAB-3420-2APW-2 Human IFN-g ELISpot PRO Kit white (ALP) (for 2 plates) 1 kit

MAB-3420-2APW-10 Human IFN-g ELISpot PRO Kit white (ALP) (for 10 plates) 1 kit

MAB-3420-2AW-Plus Human IFN-g ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3420-2H Human IFN-g ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3420-2HPT-2 Human IFN-g ELISpot PRO Kit transparent (HRP) (for 2 plates) 1 kit

MAB-3420-2HPT-10 Human IFN-g ELISpot PRO Kit transparent (HRP) (for 10 plates) 1 kit

MAB-3420-2HPW-2 Human IFN-g ELISpot PRO Kit white (HRP) (for 2 plates) 1 kit

MAB-3420-2HPW-10 Human IFN-g ELISpot PRO Kit white (HRP) (for 10 plates) 1 kit

MAB-3420-2HW-Plus Human IFN-g ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3420-2AST-2 Human IFN-g ELISpot PRO Kit (ALP) precoated strips plates (for 2 plates) 1 kit

MAB-3420-4APT-4 Human IFN-g ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3420-4APW-4 Human IFN-g ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3420-4AST-4 Human IFN-g ELISpot PLUS Kit (ALP), precoated strips plates (for 4 plates) 1 kit

MAB-3420-4HPT-4 Human IFN-g ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3420-4HPW-4 Human IFN-g ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3420-4HST-4 Human IFN-g ELISpot PLUS Kit (HRP), precoated strips plates (for 4 plates) 1 kit

MAB-3420-3-250 Anti-Human IFN-g mAb 1-D1K, purified 250 µg

MAB-3420-3-1000 Anti-Human IFN-g mAb 1-D1K, purified 1 mg

MAB-3420-6-250 Anti-Human IFN-g mAb 7-B6-1, biotinylated 250 µg

MAB-3420-6-1000 Anti-Human IFN-g mAb 7-B6-1, biotinylated 1 mg

MAB-3420-7 Anti-Human IFN-g mAb 1-D1K, FITC 250 µg

Human CEF Peptide Pool Codice Descrizione Confezione

MAB-3615-1 CEF Peptide Pool 100 µl

Human CD-3 Stimulation (positive control) Codice Descrizione Confezione

MAB-3605-1-50 Anti-CD3 mAb CD3-2, purified 500 µl

Human Interferon-alpha (subtype 2) Codice Descrizione Confezione MAB-3423-1A-6 Human IFN-a MT-1/2 ELISA (ALP) (for 6 plates) 1 kit

MAB-3423-1A-20 Human IFN-a MT-1/2 ELISA (ALP) (for 20 plates) 1 kit

MAB-3423-1H-6 Human IFN-a MT-1/2 ELISA (HRP) (for 6 plates) 1 kit


MAB-3423-2A Human IFN-a MT-1/2 ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3423-2AW-Plus Human IFN-a MT-1/2 ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3423-2H Human IFN-a MT-1/2 ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3423-2HW-Plus Human IFN-a MT-1/2 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3423-3-250 Anti-Human IFN-a mAb MT-1, purified 250 µg

MAB-3423-3-1000 Anti-Human IFN-a mAb MT-1, purified 1 mg

MAB-3423-6-250 Anti-Human IFN-a mAb MT-2, biotinylated 250 µg

MAB-3423-6-1000 Anti-Human IFN-a mAb MT-2, biotinylated 1 mg


Catalogo 2010-11



Human Interferon-alpha (subtypes 8, 10, 14, 17) Codice Descrizione Confezione MAB-3424-1A-6 Human IFN-a MT-3/4 ELISA (ALP) (for 6 plates) 1 kit

MAB-3424-1A-20 Human IFN-a MT-3/4 ELISA (ALP) (for 20 plates) 1 kit



MAB-3424-2A Human IFN-a MT-3/4 ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3424-2AW-Plus Human IFN-a MT-3/4 ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3424-2H Human IFN-a MT-3/4 ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3424-2HW-Plus Human IFN-a MT-3/4 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3424-3-250 Anti-Human IFN-a mAb MT-3, purified 250 µg

MAB-3424-3-1000 Anti-Human IFN-a mAb MT-3, purified 1 mg

MAB-3424-6-250 Anti-Human IFN-a mAb MT-4, biotinylated 250 µg

MAB-3424-6-1000 Anti-Human IFN-a mAb MT-4, biotinylated 1 mg

Human Interferon-alpha (pan specific)

Codice Descrizione Confezione MAB-3425-1A-6 Human IFN-a pan ELISA (ALP) (for 6 plates) 1 kit

MAB-3425-1A-20 Human IFN-a pan ELISA (ALP) (for 20 plates) 1 kit

MAB-3425-1H-6 Human IFN-a pan ELISA (HRP) (for 6 plates) 1 kit

MAB-3425-1H-20 Human IFN-a pan ELISA (HRP) (for 20 plates) 1 kit

MAB-3425-1HP-2 Human IFN-a ELISA PRO Kit (for 2 plates) 1 kit

MAB-3425-1HP-10 Human IFN-a ELISA PRO Kit (for 10 plates) 1 kit

MAB-3425-2A Human IFN-a pan ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3425-2AW-Plus Human IFN-a pan ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3425-2H Human IFN-a pan ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3425-2HW-Plus Human IFN-a pan ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3425-3-250 Anti-Human IFN-a mAb MT-1/-3/-5, purified 250 µg

MAB-3425-3-1000 Anti-Human IFN-a mAb MT-1/-3/-5, purified 1 mg

MAB-3425-6-250 Anti-Human IFN-a mAb MT-2/-4/-6, biotinylated 250 µg

MAB-3425-6-1000 Anti-Human IFN-a mAb MT-2/-4/-6, biotinylated 1 mg

Human Interleukin-1 beta (IL-1F2)

Codice Descrizione Confezione MAB-3415-1A-6 Human IL-1b ELISA (ALP) (for 6 plates) 1 kit

MAB-3415-1A-20 Human IL-1b ELISA (ALP) (for 20 plates) 1 kit

MAB-3415-1H-6 Human IL-1b ELISA (HRP) (for 6 plates) 1 kit

MAB-3415-1H-20 Human IL-1b ELISA (HRP) (for 20 plates) 1 kit

MAB-3415-1HP-2 Human IL-1b ELISA PRO Kit (for 2 plates) 1 kit

MAB-3415-1HP-10 Human IL-1b ELISA PRO Kit (for 10 plates) 1 kit

MAB-3415-2A Human IL-1b ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3415-2AW-Plus Human IL-1b ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3415-2H Human IL-1b ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3415-2HW-Plus Human IL-1b ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3415-3-250 Anti-Human IL-1b mAb IL1b-I, purified 250 µg

MAB-3415-3-1000 Anti-Human IL-1b mAb IL1b-I, purified 1 mg

MAB-3415-6-250 Anti-Human IL-1b mAb IL1b-II, biotinylated 250 µg

MAB-3415-6-1000 Anti-Human IL-1b mAb IL1b-II, biotinylated 1 mg


Catalogo 2010-11



Human Interleukin-2 Codice Descrizione Confezione MAB-3440-1A-6 Human IL-2 ELISA (ALP) (for 6 plates) 1 kit

MAB-3440-1A-20 Human IL-2 ELISA (ALP) (for 20 plates) 1 kit

MAB-3440-1H-6 Human IL-2 ELISA (HRP) (for 6 plates) 1 kit


MAB-3440-1HP-2 Human IL-2 ELISA PRO Kit (for 2 plates) 1 kit


MAB-3440-2A Human IL-2 ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3440-2AW-Plus Human IL-2 ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3440-2H Human IL-2 ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3440-2HW-Plus Human IL-2 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3440-4APT-4 Human IL-2 ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3440-4APW-4 Human IL-2 ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3440-4HPT-4 Human IL-2 ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3440-4HPW-4 Human IL-2 ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3440-2-250 Anti-Human IL-2 mAbs IL2-I/249, purified 250 µg

MAB-3440-2-1000 Anti-Human IL-2 mAbs IL2-I/249, purified 1 mg

MAB-3440-6-250 Anti-Human IL-2 mAb IL2-II, biotinylated 250 µg

MAB-3440-6-1000 Anti-Human IL-2 mAb IL2-II, biotinylated 1 mg








MAB-3410-2APT-2 Human IL-4 ELISpot PRO Kit transparent (for 2 plates) 1 kit

MAB-3410-2APT-10 Human IL-4 ELISpot PRO Kit transparent (for 10 plates) 1 kit

MAB-3410-2APW-2 Human IL-4 ELISpot PRO Kit white (for 2 plates) 1 kit

MAB-3410-2APW-10 Human IL-4 ELISpot PRO Kit white (for 10 plates) 1 kit




MAB-3410-4APT-4 Human IL-4 ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3410-4APW-4 Human IL-4 ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3410-4HPT-4 Human IL-4 ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3410-4HPW-4 Human IL-4 ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3410-3-250 Anti-Human IL-4 mAb IL4-I (82.4), purified 250 µg

MAB-3410-3-1000 Anti-Human IL-4 mAb IL4-I (82.4), purified 1 mg

MAB-3410-6-250 Anti-Human IL-4 mAb IL4-II (12.1), biotinylated 250 µg

MAB-3410-6-1000 Anti-Human IL-4 mAb IL4-II (12.1), biotinylated 1 mg


Catalogo 2010-11












MAB-3490-2ΗW-Plus Human IL-5 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3490-3-250 Anti-Human IL-5 mAb TRFK5, purified 250 µg

MAB-3490-3-1000 Anti-Human IL-5 mAb TRFK5, purified 1 mg

MAB-3490-6-250 Anti-Human IL-5 mAb 5A10, biotinylated 250 µg

MAB-3490-6-1000 Anti-Human IL-5 mAb 5A10, biotinylated 1 mg










MAB-3460-2ΗW-Plus Human IL-6 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3460-3-250 Anti-Human IL-6 mAb 13A5, purified 250 µg

MAB-3460-3-1000 Anti-Human IL-6 mAb 13A5, purified 1 mg

MAB-3460-6-250 Anti-Human IL-6 mAb 39C3, biotinylated 250 µg

MAB-3460-6-1000 Anti-Human IL-6 mAb 39C3, biotinylated 1 mg











MAB-3430-3-250 Anti-Human IL-10 mAb 9D7, purified 250 µg

MAB-3430-3-1000 Anti-Human IL-10 mAb 9D7, purified 1 mg

MAB-3430-6-1000 Anti-Human IL-10 mAb 12G8, biotinylated 1 mg

MAB-3430-6-250 Anti-Human IL-10 mAb 12G8, biotinylated 250 µg


Catalogo 2010-11



Human Interleukin-12 (total IL-12) Codice Descrizione Confezione MAB-3450-1A-6 Human IL-12 (total) ELISA (ALP) (for 6 plates) 1 kit

MAB-3450-1A-20 Human IL-12 (total) ELISA (ALP) (for 20 plates) 1 kit

MAB-3450-1H-6 Human IL-12 (total) ELISA (HRP) (for 6 plates) 1 kit

MAB-3450-1H-20 Human IL-12 (total) ELISA (HRP) (for 20 plates) 1 kit

MAB-3450-1HP-2 Human IL-12 (total) ELISA PRO Kit (for 2 plates) 1 kit

MAB-3450-1HP-10 Human IL-12 (total) ELISA PRO Kit (for 10 plates) 1 kit

MAB-3450-2A Human IL-12 (total) ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3450-2AW-Plus Human IL-12 (total) ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3450-2H Human IL-12 (total) ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3450-2HW-Plus Human IL-12 (total) ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3450-3-250 Anti-Human IL-12 mAb IL12-I, purified 250 µg

MAB-3450-3-1000 Anti-Human IL-12 mAb IL12-I, purified 1 mg



MAB-3450-8-250 Anti-Human IL-12 mAb IL12-III, biotinylated 250 µg

MAB-3450-8-1000 Anti-Human IL-12 mAb IL12-III, biotinylated 1 mg

Human Interleukin-12 (IL-12 p70) Codice Descrizione Confezione MAB-3455-1A-6 Human IL-12 (p70) ELISA (ALP) (for 6 plates) 1 kit

MAB-3455-1A-20 Human IL-12 (p70) ELISA (ALP) (for 20 plates) 1 kit

MAB-3455-1H-6 Human IL-12 (p70) ELISA (HRP) (for 6 plates) 1 kit

MAB-3455-1H-20 Human IL-12 (p70) ELISA (HRP) (for 20 plates) 1 kit

MAB-3455-2A Human IL-12 (p70) ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3455-2AW-Plus Human IL-12 (p70) ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3455-2H Human IL-12 (p70) ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3455-2HW-Plus Human IL-12 (p70) ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit











MAB-3470-3-250 Anti-Human IL-13 mAb IL13-I, purified 250 µg

MAB-3470-3-1000 Anti-Human IL-13 mAb IL13-I, purified 1 mg




Catalogo 2010-11



Human Interleukin-17A Codice Descrizione Confezione MAB-3520-1A-6 Human IL-17A ELISA (ALP) (for 6 plates) 1 kit

MAB-3520-1A-20 Human IL-17A ELISA (ALP) (for 20 plates) 1 kit

MAB-3520-1H-6 Human IL-17A ELISA (HRP) (for 6 plates) 1 kit

MAB-3520-1H-20 Human IL-17A ELISA (HRP) (for 20 plates) 1 kit

MAB-3520-2A Human IL-17A ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3520-2AW-Plus Human IL-17A ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3520-2H Human IL-17A ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3520-2HW-Plus Human IL-17A ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3520-3-250 Anti-Human IL-17A mAb MT44.6, purified 250 µg

MAB-3520-3-1000 Anti-Human IL-17A mAb MT44.6, purified 1 mg

MAB-3520-6-250 Anti-Human IL-17A mAb MT44.6, biotinylated 250 µg

MAB-3520-6-1000 Anti-Human IL-17A mAb MT44.6, biotinylated 1 mg

Human MIP-1b Codice Descrizione Confezione MAB-3495-1A-6 Human MIP-1b ELISA (ALP) (for 6 plates) 1 kit

MAB-3495-1A-20 Human MIP-1b ELISA (ALP) (for 20 plates) 1 kit

MAB-3495-1H-6 Human MIP-1b ELISA (HRP) (for 6 plates) 1 kit

MAB-3495-1H-20 Human MIP-1b ELISA (HRP) (for 20 plates) 1 kit

MAB-3495-1HP-2 Human MIP-1b 6 ELISA PRO Kit (for 2 plates) 1 kit

MAB-3495-1HP-10 Human MIP-1b ELISA PRO Kit (for 10 plates) 1 kit

MAB-3495-2A Human MIP-1b ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3495-2AW-Plus Human MIP-1b ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3495-2H Human MIP-1b ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3495-2HW-Plus Human MIP-1b ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3495-3-250 Anti-Human MIP-1b mAb MIP1b-I, purified 250 µg

MAB-3495-3-1000 Anti-Human MIP-1b mAb MIP1b-I, purified 1 mg

MAB-3495-6-250 Anti-Human MIP-1b mAb MIP1b-II, biotinylated 250 µg

MAB-3495-6-1000 Anti-Human MIP-1b mAb MIP1b-II, biotinylated 1 mg

Human GM-CSF Codice Descrizione Confezione MAB-3480-1A-6 Human GM-CSF ELISA (ALP) (for 6 plates) 1 kit

MAB-3480-1A-20 Human GM-CSF ELISA (ALP) (for 20 plates) 1 kit

MAB-3480-1H-6 Human GM-CSF ELISA (HRP) (for 6 plates) 1 kit

MAB-3480-1H-20 Human GM-CSF ELISA (HRP) (for 20 plates) 1 kit

MAB-3480-1HP-2 Human GM-CSF ELISA PRO Kit (for 2 plates) 1 kit

MAB-3480-1HP-10 Human GM-CSF ELISA PRO Kit (for 10 plates) 1 kit

MAB-3480-2A Human GM-CSF ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3480-2AW-Plus Human GM-CSF ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3480-2H Human GM-CSF ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3480-2HW-Plus Human GM-CSF ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3480-3-250 Anti-Human GM-CSF mAb 21C11, purified 250 µg

MAB-3480-3-1000 Anti-Human GM-CSF mAb 21C11, purified 1 mg

MAB-3480-6-250 Anti-Human GM-CSF mAb 23B6, biotinylated 250 µg

MAB-3480-6-1000 Anti-Human GM-CSF mAb 23B6, biotinylated 1 mg


Catalogo 2010-11



Human TNF-alpha Codice Descrizione Confezione MAB-3510-1A-6 Human TNF-a ELISA (ALP) (for 6 plates) 1 kit

MAB-3510-1A-20 Human TNF-a ELISA (ALP) (for 20 plates) 1 kit

MAB-3510-1H-6 Human TNF-a ELISA (HRP) (for 6 plates) 1 kit

MAB-3510-1H-20 Human TNF-a ELISA (HRP) (for 20 plates) 1 kit

MAB-3510-2A Human TNF-a ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3510-2AW-Plus Human TNF-a ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3510-2H Human TNF-a ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3510-2HW-Plus Human TNF-a ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3510-3-250 Anti-Human TNF-a mAb TNFa-I, purified 250 µg

MAB-3510-3-1000 Anti-Human TNF-a mAb TNFa-I, purified 1 mg

MAB-3510-6-250 Anti-Human TNF-a mAb TNFa-II, biotinylated 250 µg

MAB-3510-6-1000 Anti-Human TNF-a mAb TNFa-II, biotinylated 1 mg

Human Perforin

Codice Descrizione Confezione MAB-3465-1A-6 Human Perforin ELISA (ALP) (for 6 plates) 1 kit

MAB-3465-1A-20 Human Perforin ELISA (ALP) (for 20 plates) 1 kit

MAB-3465-1H-6 Human Perforin ELISA (HRP) (for 6 plates) 1 kit

MAB-3465-1H-20 Human Perforin ELISA (HRP) (for 20 plates) 1 kit

MAB-3465-1HP-2 Human Perforin ELISA PRO Kit (for 2 plates) 1 kit

MAB-3465-1HP-10 Human Perforin ELISA PRO Kit (for 10 plates) 1 kit

MAB-3465-2A Human Perforin ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3465-2AW-Plus Human Perforin ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3465-2H Human Perforin ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3465-2HW-Plus Human Perforin ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3465-4APT-4 Human Perforin ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3465-4APW-4 Human Perforin ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3465-4HPT-4 Human Perforin ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3465-4HPW-4 Human Perforin ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3465-3-250 Anti-Human Perforin mAbs Pf-80/164, purified 500 µg

MAB-3465-3-1000 Anti-Human Perforin mAbs Pf-80/164, purified 2 mg

MAB-3465-6-250 Anti-Human Perforin mAb Pf-344, biotinylated 250 µg

MAB-3465-6-1000 Anti-Human Perforin mAb Pf-344, biotinylated 1 mg

MAB-3465-7 Anti-Human Perforin mAb Pf-344, FITC 250 µg

Human Granzyme B Codice Descrizione Confezione MAB-3485-1A-6 Human Granzyme B ELISA (ALP) (for 6 plates) 1 kit

MAB-3485-1A-20 Human Granzyme B ELISA (ALP) (for 20 plates) 1 kit

MAB-3485-1H-6 Human Granzyme B ELISA (HRP) (for 6 plates) 1 kit

MAB-3485-1H-20 Human Granzyme B ELISA (HRP) (for 20 plates) 1 kit

MAB-3485-2A Human Granzyme B ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3485-2AW-Plus Human Granzyme B ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3485-2H Human Granzyme B ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3485-2HW-Plus Human Granzyme B ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3485-3-250 Anti-Human Granzyme B mAb GB10, purified 250 µg

MAB-3485-3-1000 Anti-Human Granzyme B mAb GB10, purified 1 mg

MAB-3485-6-250 Anti-Human Granzyme B mAb GB11, biotinylated 250 µg

MAB-3485-6-1000 Anti-Human Granyzme B mAb GB11, biotinylated 1 mg


Catalogo 2010-11


MabTech: Monkey ELISpot & ELISA Kits

Monkey Interferon-gamma Codice Descrizione Confezione MAB-3420M-1A-6 Monkey IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3420M-1A-20 Monkey IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3420M-1H-6 Monkey IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3420M-1H-20 Monkey IFN-g ELISA (HRP) (for 20 plates) 1 kit

MAB-3420M-1HP-2 Monkey IFN-g ELISA PRO Kit (for 2 plates) 1 kit

MAB-3420M-1HP-10 Monkey IFN-g ELISA PRO Kit (for 10 plates) 1 kit

MAB-3420M-2A Monkey IFN-g ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3420M-2APT-2 Monkey IFN-g ELISpot PRO Kit transparent (for 2 plates) 1 kit

MAB-3420M-2APT-10 Monkey IFN-g ELISpot PRO Kit transparent (for 10 plates) 1 kit

MAB-3420M-2AST-2 Monkey IFN-g ELISpot PRO Kit transparent strips plates (for 2 plates) 1 kit

MAB-3420M-2APW-2 Monkey IFN-g ELISpot PRO Kit white (for 2 plates) 1 kit

MAB-3420M-2APW-10 Monkey IFN-g ELISpot PRO Kit white (for 10 plates) 1 kit

MAB-3420M-2AW-Plus Monkey IFN-g ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3420M-2H Monkey IFN-g ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3420M-2HPT-2 Monkey IFN-g ELISpot PRO Kit transparent (HRP) (for 2 plates) 1 kit

MAB-3420M-2HPT-10 Monkey IFN-g ELISpot PRO Kit transparent (HRP) (for 10 plates) 1 kit

MAB-3420M-2HPW-2 Monkey IFN-g ELISpot PRO Kit white (HRP) (for 2 plates) 1 kit

MAB-3420M-2HPW-10 Monkey IFN-g ELISpot PRO Kit white (HRP) (for 10 plates) 1 kit

MAB-3420M-2HW-Plus Monkey IFN-g ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3420M-4APT-4 Monkey IFN-g ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3420M-4APW-4 Monkey IFN-g ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3420M-4AST-4 Monkey IFN-g ELISpot PLUS Kit (ALP), transparent strips plates (for 4 plates) 1 kit

MAB-3420M-4HPT-4 Monkey IFN-g ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3420M-4HPW-4 Monkey IFN-g ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3420M-4HST-4 Monkey IFN-g ELISpot PLUS Kit (HRP), transparent strips plates (for 4 plates) 1 kit

MAB-3420M-3-250 Anti-Human Monkey IFN-g mAb GZ-4, purified 250 µg

MAB-3420M-3-1000 Anti-Human Monkey IFN-g mAb GZ-4, purified 1 mg

MAB-3420-6-250 Anti-Human Monkey IFN-g mAb 7-B6-1, biotinylated 250 µg

MAB-3420-6-1000 Anti-Human Monkey IFN-g mAb 7-B6-1, biotinylated 1 mg

Monkey Interleukin-2

Codice Descrizione Confezione MAB-3440M-1A-6 Monkey IL-2 ELISA (ALP) (for 6 plates) 1 kit

MAB-3440M-1A-20 Monkey IL-2 ELISA (ALP) (for 20 plates) 1 kit

MAB-3440M-1H-6 Monkey IL-2 ELISA (HRP) (for 6 plates) 1 kit

MAB-3440M-1H-20 Monkey IL-2 ELISA (HRP) (for 20 plates) 1 kit

MAB-3440M-1HP-2 Monkey IL-2 ELISA PRO Kit (for 2 plates) 1 kit

MAB-3440M-1HP-10 Monkey IL-2 ELISA PRO Kit (for 10 plates) 1 kit

MAB-3440M-2A Monkey IL-2 ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3440M-2AW-Plus Monkey IL-2 ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3440M-2H Monkey IL-2 ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3440M-2HW-Plus Monkey IL-2 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3440M-4APT-4 Monkey IL-2 ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3440M-4APW-4 Monkey IL-2 ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3440M-4HPT-4 Monkey IL-2 ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3440M-4HPW-4 Monkey IL-2 ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3440M-2-250 Anti-Human Monkey IL-2 mAb IL2M-I/249, purified 250 µg

MAB-3440M-2-1000 Anti-Human Monkey IL-2 mAb IL2M-I/249, purified 1 mg

MAB-3440-6-250 Anti-Human Monkey IL-2 mAb IL2-II, biotinylated 250 µg

MAB-3440-6-1000 Anti-Human Monkey IL-2 mAb IL2-II, biotinylated 1 mg


Catalogo 2010-11


MabTech: Monkey ELISpot & ELISA Kits

Monkey Interleukin-17a Codice Descrizione Confezione MAB-3520M-1A-6 Monkey IL-17A ELISA (ALP) (for 6 plates) 1 kit

MAB-3520M-1A-20 Monkey IL-17A ELISA (ALP) (for 20 plates) 1 kit

MAB-3520M-1H-6 Monkey IL-17A ELISA (HRP) (for 6 plates) 1 kit

MAB-3520M-1H-20 Monkey IL-17A ELISA (HRP) (for 20 plates) 1 kit

MAB-3520M-2A Monkey IL-17A ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3520M-2AW-Plus Monkey IL-17A ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3520M-2H Monkey IL-17A ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3520M-2HW-Plus Monkey IL-17A ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3520M-3-250 Anti-Human Monkey IL-17A mAb MT241, purified 250 µg

MAB-3520M-3-1000 Anti-Human Monkey IL-17A mAb MT241, purified 1 mg

MAB-3520-6-250 Anti-Human IL-17A mAb MT504, biotinylated 250 µg

MAB-3520-6-1000 Anti-Human IL-17A mAb MT504, biotinylated 1 mg

Monkey CD-3 Stimulation (positive control) Vedi Human CD-3 Stimulation (positive control), pagina 33


Vedi Human Interleukin-4, pagina 35





Monkey Interleukin-12 (total) Vedi Human Interleukin-12 (total), pagina 37



Monkey GM-CSF

Vedi Human GM-CSF, pagina 38

Monkey Perforin

Vedi Human Perforin, pagina 39


Catalogo 2010-11


MabTech: Mouse ELISpot & ELISA Kits

Mouse Interferon-gamma Codice Descrizione Confezione MAB-3321-1A-6 Mouse IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3321-1A-20 Mouse IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3321-1H-6 Mouse IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3321-1H-20 Mouse IFN-g ELISA (HRP) (for 20 plates) 1 kit

MAB-3321-1HP-2 Mouse IFN-g ELISA PRO Kit (for 2 plates) 1 kit

MAB-3321-1HP-10 Mouse IFN-g ELISA PRO Kit (for 10 plates) 1 kit

MAB-3321-2A Mouse IFN-g ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3321-2AW-Plus Mouse IFN-g ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3321-2H Mouse IFN-g ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3321-2HW-Plus Mouse IFN-g ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3321-4APT-4 Mouse IFN-g ELISpot PLUS Kit (ALP), precoated transparent plates (for 4 plates) 1 kit

MAB-3321-4APW-4 Mouse IFN-g ELISpot PLUS Kit (ALP), precoated white plates (for 4 plates) 1 kit

MAB-3321-4HPT-4 Mouse IFN-g ELISpot PLUS Kit (HRP), precoated transparent plates (for 4 plates) 1 kit

MAB-3321-4HPW-4 Mouse IFN-g ELISpot PLUS Kit (HRP), precoated white plates (for 4 plates) 1 kit

MAB-3321-3-250 Anti-Mouse IFN-g mAb AN18, purified 250 µg

MAB-3321-3-1000 Anti-Mouse IFN-g mAb AN18, purified 1 mg

MAB-3321-6-250 Anti-Mouse IFN-g mAb R4-6A2, biotinylated 250 µg

MAB-3321-6-1000 Anti-Mouse IFN-g mAb R4-6A2, biotinylated 1 mg

Mouse Interleukin-2

Codice Descrizione Confezione MAB-3441-1A-6 Mouse IL-2 ELISA (ALP) (for 6 plates) 1 kit

MAB-3441-1A-20 Mouse IL-2 ELISA (ALP) (for 20 plates) 1 kit

MAB-3441-1H-6 Mouse IL-2 ELISA (HRP) (for 6 plates) 1 kit


MAB-3441-1HP-2 Mouse IL-2 ELISA PRO Kit (for 2 plates) 1 kit


MAB-3441-2A Mouse IL-2 ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3441-2AW-Plus Mouse IL-2 ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3441-2H Mouse IL-2 ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3441-2HW-Plus Mouse IL-2 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3441-3-250 Anti-Mouse IL-2 mAb 1A12, purified 250 µg

MAB-3441-3-1000 Anti-Mouse IL-2 mAb 1A12, purified 1 mg

MAB-3441-6-250 Anti-Mouse IL-2 mAb 5H4, biotinylated 250 µg

MAB-3441-6-1000 Anti-Mouse IL-2 mAb 5H4, biotinylated 1 mg

Mouse Interleukin-4











MAB-3311-3-250 Anti-Mouse IL-4 mAb 11B11, purified 250 µg

MAB-3311-3-1000 Anti-Mouse IL-4 mAb 11B11, purified 1 mg

MAB-3311-6-250 Anti-Mouse IL-4 mAb BVD6-24G2, biotinylated 250 µg

MAB-3311-6-1000 Anti-Mouse IL-4 mAb BVD6-24G2, biotinylated 1 mg


Catalogo 2010-11



Mouse Interleukin-5 Codice Descrizione Confezione MAB-3391-1A-6 Mouse IL-5 ELISA (ALP) (for 6 plates) 1 kit










MAB-3391-3-250 Anti-Mouse IL-5 mAb TRFK5, purified 250 µg

MAB-3391-3-1000 Anti-Mouse IL-5 mAb TRFK5, purified 1 mg

MAB-3391-6-250 Anti-Mouse IL-5 mAb TRFK4, biotinylated 250 µg

MAB-3391-6-1000 Anti-Mouse IL-5 mAb TRFK4, biotinylated 1 mg

Mouse Interleukin-10









MAB-3431-3-250 Anti-Mouse IL-10 mAb 16E3, purified 250 µg

MAB-3431-3-1000 Anti-Mouse IL-10 mAb 16E3, purified 1 mg

MAB-3431-4-250 Anti-Mouse IL-10 mAb 16E3, biotinylated 250 µg

MAB-3431-4-1000 Anti-Mouse IL-10 mAb 16E3, biotinylated 1 mg

MAB-3431-5-250 Anti-Mouse IL-10 mAb 2°5, purified 250 µg

MAB-3431-5-1000 Anti-Mouse IL-10 mAb 2°5, purified 1 mg

MAB-3431-6-250 Anti-Mouse IL-10 mAb 2A5, biotinylated 250 µg

MAB-3431-6-1000 Anti-Mouse IL-10 mAb 2A5, biotinylated 1 mg

Mouse Interleukin-12 (total IL-12)

Codice Descrizione Confezione MAB-3451-1A-6 Mouse IL-12 (total) ELISA (ALP) (for 6 plates) 1 kit

MAB-3451-1A-20 Mouse IL-12 (total) ELISA (ALP) (for 20 plates) 1 kit

MAB-3451-1H-6 Mouse IL-12 (total) ELISA (HRP) (for 6 plates) 1 kit

MAB-3451-1H-20 Mouse IL-12 (total) ELISA (HRP) (for 20 plates) 1 kit

MAB-3451-1HP-2 Mouse IL-12 (total) ELISA PRO Kit (for 2 plates) 1 kit

MAB-3451-1HP-10 Mouse IL-12 (total) ELISA PRO Kit (for 10 plates) 1 kit

MAB-3451-2A Mouse IL-12 (total) ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3451-2AW-Plus Mouse IL-12 (total) ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3451-2H Mouse IL-12 (total) ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3451-2HW-Plus Mouse IL-12 (total) ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3451-3-250 Anti-Mouse IL-12 mAb C15.6, purified 250 µg

MAB-3451-3-1000 Anti-Mouse IL-12 mAb C15.6, purified 1 mg

MAB-3451-6-250 Anti-Mouse IL-12 mAb C17.8, biotinylated 250 µg

MAB-3451-6-1000 Anti-Mouse IL-12 mAb C17.8, biotinylated 1 mg


Catalogo 2010-11



Mouse Interleukin-12 (IL-12 p70) Codice Descrizione Confezione MAB-3456-1A-6 Mouse IL-12 (p70) ELISA (ALP) (for 6 plates) 1 kit

MAB-3456-1A-20 Mouse IL-12 (p70) ELISA (ALP) (for 20 plates) 1 kit

MAB-3456-1H-6 Mouse IL-12 (p70) ELISA (HRP) (for 6 plates) 1 kit

MAB-3456-1H-20 Mouse IL-12 (p70) ELISA (HRP) (for 20 plates) 1 kit

MAB-3456-1HP-2 Mouse IL-12 (p70) ELISA PRO Kit (for 2 plates) 1 kit

MAB-3456-1HP-10 Mouse IL-12 (p70) ELISA PRO Kit (for 10 plates) 1 kit

MAB-3456-2A Mouse IL-12 (p70) ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3456-2AW-Plus Mouse IL-12 (p70) ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3456-2H Mouse IL-12 (p70) ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3456-2HW-Plus Mouse IL-12 (p70) ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3456-3-250 Anti-Mouse IL-12 mAb C17.8, purified 250 µg

MAB-3456-3-1000 Anti-Mouse IL-12 mAb C17.8, purified 1 mg

MAB-3456-6-250 Anti-Mouse IL-12 mAb IL12-IV, biotinylated 250 µg

MAB-3456-6-1000 Anti-Mouse IL-12 mAb IL12-IV, biotinylated 1 mg

Mouse Interleukin-17A Codice Descrizione Confezione MAB-3521-1A-6 Mouse IL-17A ELISA (ALP) (for 6 plates) 1 kit

MAB-3521-1A-20 Mouse IL-17A ELISA (ALP) (for 20 plates) 1 kit

MAB-3521-1H-6 Mouse IL-17A ELISA (HRP) (for 6 plates) 1 kit

MAB-3521-1H-20 Mouse IL-17A ELISA (HRP) (for 20 plates) 1 kit

MAB-3521-2A Mouse IL-17A ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3521-2AW-Plus Mouse IL-17A ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3521-2H Mouse IL-17A ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3521-2HW-Plus Mouse IL-17A ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3521-3-250 Anti-Mouse IL-17A mAb IL17A-I, purified 250 µg

MAB-3521-3-1000 Anti-Mouse IL-17A mAb IL17A-I, purified 1 mg

MAB-3521-6-250 Anti-Mouse IL-17A mAb IL17A-II, biotinylated 250 µg

MAB-3521-6-1000 Anti-Mouse IL-17A mAb IL17A-II, biotinylated 1 mg


Catalogo 2010-11


MabTech: Others ELISpot & ELISA Kits

Bovine Interferon-gamma Codice Descrizione Confezione MAB-3115-1A-6 Bovine IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3115-1A-20 Bovine IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3115-1H-6 Bovine IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3115-1H-20 Bovine IFN-g ELISA (HRP) (for 20 plates) 1 kit

MAB-3115-2A Bovine/Ovine/Equine IFN-g ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3115-2AW-Plus Bovine/Ovine/Equine IFN-g ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3115-2H Bovine/Ovine/Equine IFN-g ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3115-2HW-Plus Bovine/Ovine/Equine IFN-g ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3115-3-250 Anti-Bovine IFN-g mAb bIFNg-I, purified 250 µg

MAB-3115-3-1000 Anti-Bovine IFN-g mAb bIFNg-I, purified 1 mg

MAB-3115-6-125 Anti-Bovine IFN-g mAb PAN, biotinylated 125 µg


MAB-3115-7 Anti-Bovine IFN-g mAb PAN, FITC 500 µg

Ovine Interferon-gamma

Codice Descrizione Confezione MAB-3116-1A-6 Ovine IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3116-1A-20 Ovine IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3116-1H-6 Ovine IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3116-1H-20 Ovine IFN-g ELISA (HRP) (for 20 plates) 1 kit










Equine Interferon-gamma

Codice Descrizione Confezione MAB-3117-1A-6 Equine IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3117-1A-20 Equine IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3117-1H-6 Equine IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3117-1H-20 Equine IFN-g ELISA (HRP) (for 20 plates) 1 kit











Catalogo 2010-11


MabTech: Others ELISpot & ELISA Kits

Porcine Interferon-gamma Codice Descrizione Confezione MAB-3130-1A-6 Porcine IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3130-1A-20 Porcine IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3130-1H-6 Porcine IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3130-1H-20 Porcine IFN-g ELISA (HRP) (for 20 plates) 1 kit

MAB-3130-2A Porcine IFN-g ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3130-2AW-Plus Porcine IFN-g ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3130-2H Porcine IFN-g ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3130-2HW-Plus Porcine IFN-g ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3130-3-250 Anti-Porcine IFN-g mAb POR, purified 250 µg

MAB-3130-3-1000 Anti-Porcine IFN-g mAb POR, purified 1 mg




Rat Interferon-gamma Codice Descrizione Confezione MAB-3220-1A-6 Rat IFN-g ELISA (ALP) (for 6 plates) 1 kit

MAB-3220-1A-20 Rat IFN-g ELISA (ALP) (for 20 plates) 1 kit

MAB-3220-1H-6 Rat IFN-g ELISA (HRP) (for 6 plates) 1 kit

MAB-3220-1H-20 Rat IFN-g ELISA (HRP) (for 20 plates) 1 kit

MAB-3220-2A Rat IFN-g ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3220-2AW-Plus Rat IFN-g ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3220-2H Rat IFN-g ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3220-2HW-Plus Rat IFN-g ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3220-3-250 Anti-Rat IFN-g mAb rIFNg-I, purified 250 µg

MAB-3220-3-1000 Anti-Rat IFN-g mAb rIFNg-I, purified 1 mg

MAB-3220-6-250 Anti-Rat IFN-g mAb rIFNg-II, biotinylated 250 µg

MAB-3220-6-1000 Anti-Rat IFN-g mAb rIFNg-II, biotinylated 1 mg


Catalogo 2010-11


MabTech: Altri (Streptavidin, Anti-CD, etc)

Human CD3 Codice Descrizione Confezione MAB-3605-1-250 Anti-CD3 mAb CD3-2, purified 250 µg

MAB-3605-1-1000 Anti-CD3 mAb CD3-2, purified 1 mg

MAB-3605-2 Anti-CD3 mAb CD3-2, FITC 100 tests

MAB-3605-3 Anti-CD3 mAb CD3-2, rhodamine 100 tests

Human CD4

Codice Descrizione Confezione MAB-3606-1-250 Anti-CD4 mAb CD4-1, purified 250 µg




Human CD8

Codice Descrizione Confezione MAB-3607-1-250 Anti-CD8 mAb CD8-1, purified 250 µg




Human CD40

Codice Descrizione Confezione MAB-3601-1-250 Anti-CD40 mAb S2C6, purified 250 µg

MAB-3601-1-1000 Anti-CD40 mAb S2C6, purified 1 mg

MAB-3601-2-250 Anti-CD40 mAb S2C6, biotinylated 250 µg

MAB-3601-2-1000 Anti-CD40 mAb S2C6, biotinylated 1 mg

MAB-3601-3 Anti-CD40 mAb S2C6, FITC 100 tests

MAB-3603-1-250 Anti-CD40 mAb 17:40, purified 250 µg

MAB-3603-1-1000 Anti-CD40 mAb 17:40, purified 1 mg

Human CD43

Codice Descrizione Confezione MAB-3602-1-250 Anti-CD43 mAb B1B6, purified 250 µg

MAB-3602-1-1000 Anti-CD43 mAb B1B6, purified 1 mg

MAB-3602-2-250 Anti-CD43 mAb B1B6, biotinylated 250 µg

MAB-3602-2-1000 Anti-CD43 mAb B1B6, biotinylated 1 mg

MAB-3602-3 Anti-CD43 mAb B1B6, FITC 100 tests

Human Insulin

Codice Descrizione Confezione MAB-3120-3-250 Anti-Human Insulin mAb E2-E3, purified 250 µg

MAB-3120-3-1000 Anti-Human Insulin mAb E2-E3, purified 1 mg

MAB-3120-6-250 Anti-Human Insulin mAb 2D11-H5, biotinylated 250 µg

MAB-3120-6-1000 Anti-Human Insulin mAb 2D11-H5, biotinylated 1 mg


Catalogo 2010-11



Human Apolipoprotein A1 (ApoA1) Codice Descrizione Confezione MAB-3710-1A-6 Human ApoA1 ELISA Kit (ALP) (for 6 plates) 1 kit

MAB-3710-1A-20 Human ApoA1 ELISA Kit (ALP) (for 20 plates) 1 kit

MAB-3710-1H-6 Human ApoA1 ELISA Kit (HRP) (for 6 plates) 1 kit

MAB-3710-1H-20 Human ApoA1 ELISA Kit (HRP) (for 20 plates) 1 kit

MAB-3710-1HP-2 Human ApoA1 ELISA PRO Kit (for 2 plates) 1 kit

MAB-3710-1HP-10 Human ApoA1 ELISA PRO Kit (for 10 plates) 1 kit

MAB-3710-2A Human ApoA1 ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3710-2AW-Plus Human ApoA1 ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3710-2H Human ApoA1 ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3710-2HW-Plus Human ApoA1 ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3710-3-250 Anti-ApoA1 mAb HDL 110, purified 250 µg

MAB-3710-3-1000 Anti-ApoA1 mAb HDL 110, purified 1 mg

MAB-3710-6-250 Anti-ApoA1 mAb HDL 44, biotinylated 250 µg

MAB-3710-6-1000 Anti-ApoA1 mAb HDL 44, biotinylated 1 mg

MAB-3710-7 Anti-ApoA1 mAb HDL 44, FITC 100 µg

MAB-3710-8 Anti-ApoA1 mAb HDL 44, rhodamine 100 µg

Human Apolipoprotein B (ApoB)

Codice Descrizione Confezione MAB-3715-1A-6 Human ApoB ELISA Kit (ALP) (for 6 plates) 1 kit

MAB-3715-1A-20 Human ApoB ELISA Kit (ALP) (for 20 plates) 1 kit

MAB-3715-1H-6 Human ApoB ELISA Kit (HRP) (for 6 plates) 1 kit

MAB-3715-1H-20 Human ApoB ELISA Kit (HRP) (for 20 plates) 1 kit

MAB-3715-1HP-2 Human ApoB ELISA PRO Kit (for 2 plates) 1 kit

MAB-3715-1HP-10 Human ApoB ELISA PRO Kit (for 10 plates) 1 kit

MAB-3715-2A Human ApoB ELISpot Kit (ALP) (for 4 plates) 1 kit

MAB-3715-2AW-Plus Human ApoB ELISpot PLUS Kit (ALP) (for 4 plates) 1 kit

MAB-3715-2H Human ApoB ELISpot Kit (HRP) (for 4 plates) 1 kit

MAB-3715-2HW-Plus Human ApoB ELISpot PLUS Kit (HRP) (for 4 plates) 1 kit

MAB-3715-3-250 Anti-ApoB mAb LDL 20/17, purified 250 µg

MAB-3715-3-1000 Anti-ApoB mAb LDL 20/17, purified 1 mg

MAB-3715-6-250 Anti-ApoB mAb LDL 11, biotinylated 250 µg

MAB-3715-6-1000 Anti-ApoB mAb LDL 11, biotinylated 1 mg

MAB-3715-7 Anti-ApoB mAb LDL 11, FITC 100 µg

MAB-3715-8 Anti-ApoB mAb LDL 11, rhodamine 100 µg

Human Apolipoprotein E (ApoE)

Codice Descrizione Confezione MAB-3712-1A-6 Human ApoE ELISA Kit (ALP) (for 6 plates) 1 kit

MAB-3712-1A-20 Human ApoE ELISA Kit (ALP) (for 20 plates) 1 kit

MAB-3712-1H-6 Human ApoE ELISA Kit (HRP) (for 6 plates) 1 kit

MAB-3712-1H-20 Human ApoE ELISA Kit (HRP) (for 20 plates) 1 kit

MAB-3712-3-250 Anti-ApoE mAb E276, purified 250 µg

MAB-3712-3-1000 Anti-ApoE mAb E276, purified 1 mg

MAB-3712-6-250 Anti-ApoE mAb E887, biotinylated 250 µg

MAB-3712-6-1000 Anti-ApoE mAb E887, biotinylated 1 mg


Catalogo 2010-11



Human Xanthine Oxidase Codice Descrizione Confezione MAB-3604-3-250 Anti-Xanthine Oxidase mAb X1-7, purified 250 µg

MAB-3604-3-1000 Anti-Xanthine Oxidase mAb X1-7, purified 1 mg

Human Amyloid-β

Codice Descrizione Confezione MAB-3740-5-100 Anti-Human Amyloid-β mAb bm-AbetaN, purified 100 µg

MAB-3740-5-250 Anti-Human Amyloid-β mAb bm-AbetaN, purified 250 µg

MAB-3740-5-1000 Anti-Human Amyloid-β mAb bm-AbetaN, purified 1 mg

MAB-3740-6-100 Anti-Human Amyloid-β mAb bm-AbetaN, biotinylated 100 µg

MAB-3740-6-250 Anti-Human Amyloid-β mAb bm-AbetaN, biotinylated 250 µg

MAB-3740-6-1000 Anti-Human Amyloid-β mAb bm-AbetaN, biotinylated 1 mg

Streptavidin-ALP/HRP

Codice Descrizione Confezione MAB-3310-8 Streptavidin-ALP 1 ml

MAB-3310-9 Streptavidin-HRP 1 ml

MAB-3310-10 Streptavidin-ALP for ELISpot 1 ml

IgE

Codice Descrizione Confezione MAB-3810-3-250 Anti-Human ΙgΕ mAb 107, purified 250 µg

MAB-3810-3-1000 Anti-Human ΙgΕ mAb 107, purified 1 mg

MAB-3810-6-250 Anti-Human ΙgΕ mAb 182, biotinylated 250 µg

MAB-3810-6-1000 Anti-Human ΙgΕ mAb 182, biotinylated 1 mg

MAB-3810-1HP-2 Human IgE ELISA PRO Kit (for 2 plates) 1 kit

MAB-3810-1HP-10 Human IgE ELISA PRO Kit (for 10 plates) 1 kit

MAB-3810-1A-6 Human IgE ELISA kit (ALP) for 6 plates

MAB-3810-1A-20 Human IgE ELISA kit (ALP) for 20 plates

MAB-3810-1H-6 Human IgE ELISA kit (HRP) for 6 plates

MAB-3810-1H-20 Human IgE ELISA kit (HRP) for 20 plates

Antigen-specific IgE detection reagent

Codice Descrizione Confezione MAB-3810-7-250 Anti-Human ΙgΕ mAbs 107/182/101, purified 250 µg

MAB-3810-7-1000 Anti-Human ΙgΕ mAbs 107/182/101, purified 1 mg

MAB-3810-8-250 Anti-Human ΙgΕ mAbs 107/182/101, biotinylated 250 µg

MAB-3810-8-1000 Anti-Human ΙgΕ mAbs 107/182/101, biotinylated 1 mg

Anti-Ig-Enzyme Conjugates (ALP)

Codice Descrizione Confezione MAB-3310-1 Goat Anti-Human IgG-ALP (IgG-specific) 1 ml

MAB-3310-2 Goat Anti-Human IgM-ALP (IgM-specific) 1 ml

MAB-3310-3 Goat Anti-Human IgA-ALP (IgA-specific) 1 ml

MAB-3310-4 Goat Anti-Mouse IgG-ALP (IgG-specific) 1 ml

MAB-3310-5 Goat Anti-Mouse IgG+IgM-ALP 1 ml

MAB-3310-6 Goat Anti-Rat IgG-ALP 1 ml

MAB-3310-7 Goat Anti-Rabbit IgG-ALP 1 ml


Catalogo 2010-11



Ready-to-use substrates Codice Descrizione Confezione MAB-3650-10 BCIP/NBT substrate 125 ml

MAB-3651-10 TMB substrate 125 ml

Plate-substrate-4-Pack Codice Descrizione Confezione MAB-3653-MWB-4 4 white MSIP plates + BCIP/NBT substrate 4 plates, substrate

MAB-3653-MTB-4 4 transp. MSIP plates + BCIP/NBT substrate 4 plates, substrate

MAB-3653-EWB-4 4 white ELIIP plates + BCIP/NBT substrate 4 plates, substrate

MAB-3653-MWT-4 4 white MSIP plates + TMB substrate 4 plates, substrate

MAB-3653-MTT-4 4 transp. MSIP plates +TMB substrate 4 plates, substrate

MAB-3653-EWT-4 4 white ELIIP plates + TMB substrate 4 plates, substrate

ELISA diluent Codice Descrizione Confezione MAB-3652-D2 Ready-to-use ELISA diluent 2 x 120 ml


Catalogo 2010-11


ELISA Kit per lo studio delle implicazioni di ormoni, citochine e altri fattori

ELISA Kits

Human ELISA Kits: Apoptosi Alcool Test Amine Biogeniche Cardiologia – Pressione Sanguigna Citochine e Chemochine Diabetologia – Molecole di Regolazione Pancreatica Droghe d’Abuso Farmaci Biologici – Nuovi Farmaci Fertilità Gastroenterologia Immunologia Infezione e Infiammazione Intolleranze Alimentari\ Malattie Infettive Metabolismo del Ferro Metabolismo Energetico e Lipidico Metabolismo Osseo Metallo Proteinasi Molecole di Adesione Nefrologia Neurologia Oncologia Ormoni Rapid Test Stress Ossidativo Vitamine

Studi Animali

Elenco Completo disponibile sul Catalogo Diagnostica 2009-10 oppure sul nostro sito sotto la categoria “Diagnostica”.


Catalogo 2010-11



Catalogo 2010-11


Sistemi magnetici: Ademtech Proteomica 54

Proteina A e G Ig Adem kit Histidin Adem kit

Epigenetica 57

ChIP-Adem-Kit

Cell Sorting 58

Bio-Adembeads Viro-Adembeads

Genomics 61

Direct mRNA purification kit Separazione DNA Smart D-N-Adem kit

Supporti e apparati magnetici 64 Sviluppo di Immunoassay 65 Reagenti e soluzioni 66


Catalogo 2010-11


Proteomica: Bio-Adembeads Protein A/G Caratteristiche:

• Le Bio-Adembeads Protein A/G forniscono un sistema molto semplice per l’immunoprecipitazione di proteine e purificazione di anticorpi

• Le nanoparticelle magnetiche, a forma perfettamente sferica ed elevata superficie disponibile, sono coniugate a proteina A o G in grado di legare esclusivamente le Ig

• La fase di preclearing non è necessaria e il background è molto ridotto • L’anticorpo può venire immobilizzato sulle nanoparticelle (crosslinking) mediante trattamento con DMP

Procedura:

Risultati:

Vantaggi:

• Applicazioni illimitate con qualsiasi anticorpo • Tecnologia Magnetica: Alta specificità, nessun background • Rapidità di esecuzione: Meno di 1 ora • Nessuna eluizione di IgG: Procedura per IgG crosslinking • Elevata capacità: Fino a 100 test per 2ml di prodotto • Semplicità: Nessuna centrifugazione. Si evita il passaggio di preclearing. Limitato numero di lavaggi

Prodotto Diametro particelle (nm) Volume Codice

300 2 ml ADE-04230 Proteina A

300 5 ml ADE-04231

300 2 ml ADE-04330 Proteina G

300 5 ml ADE-04331

300 2 ml ADE-04630 Proteina A/G

300 5 ml ADE-04631


Catalogo 2010-11


Proteomica: Bio-Ig Adem-kit Caratteristiche:

• Ig Adem-kit offre una gamma completa di soluzioni per la purificazione di immunoglobuline da siero, sovranatanti di colture di ibridomi e asciti

• La procedura semplice e rapida non richiede la rigenerazione delle nanoparticelle magnetiche che sono aliquotate e pronte all’uso

• Alta affinità di legame • Versatile: si può applicare a Ig di varie specie: uomo, topo, ratto, coniglio e pollo

Procedura:

Risultati:

Vantaggi:

• Purificazione delle Immunoglobuline totali: IgG, IgM, IgA • Elevata capacità di legame: Per purificazione su larga scala › 15 mg di IgG/ml di particelle magnetiche • Ampia Specificità: Applicabile a siero, asciti o surnatante di numerose specie • Semplice da usare • Rapido: Meno di 30 minuti

Prodotto Descrizione Volume Codice

15 purificazioni, fino a 4 mg di Ig

ADE-04400 Ig Adem-Kit Per purificazione Ig

2 x 15 purificazioni, fino a 4 mg di Ig

ADE-04401


Catalogo 2010-11


Proteomica: Bio-Histidine Adem-kit Caratteristiche:

• Sistema adatto alla purificazione di proteine ricombinanti • L’affinità dell’istidina per il nichel di cui sono ricoperte le nanoparticelle magnetiche consente la purificazione

selettiva di proteine di fusione con istidina • Alta affinità di legame con un background ridotto • Tecnologia magnetica che permette di concentrare la proteina target attraverso l’utilizzo di biglie magnetiche

"pre-caricate" con Nichel Procedura:

Risultati:

Vantaggi:

• Elevata capacità di legame: 4 mg/ml. • Flessibilità: E' possibile adattare il volume delle biglie alla quantità del campione. • Convenienza: Sistema economico. • Versatilità: Può essere usato con i normali supporti magnetici e con il sistema semi-automatico AutoMag. • Semplicità: Le proteine His-Tag possono essere eluite dalle biglie magnetiche grazie a buffers contenenti

imidazolo.


Histidine Adem-Kit Per purificazione di

proteine ricombinanti 2 ml per 65 purificazioni

fino a 60 µg 6XHis-tagged protein ADE-04500


Catalogo 2010-11


Epigenetica: ChIP-Adem-Kit Il ChIP-Adem-Kit è un sistema innovativo, concepito specificamente per lo studio delle interazioni tra fattori di trascrizione o istoni e DNA. Le nanoparticelle rivestite di proteina A o G, dopo incubazione con il Blocking Buffer, posseggono una specifica superficie, progettata per ottenere un’ efficiente immunoprecipitazione del complesso Proteina/DNA e per minimizzare l’aspecificità. Proteine ed altri contaminanti vengono eliminati durante i passaggi di lavaggio. Il protocollo per ChIP viene notevolmente semplificato, usando le nanoparticelle di Proteina A e G, legate covalentemente al DNA. Alcuni passaggi sono stati completamente eliminati. A differenza delle tradizionali particelle di agarosio, la fase di pre-clearing non è necessaria. Con le ChIP-Adembeads associate al Blocking buffer si riduce il background e si massimizza la quantità di templato disponibile per la reazione, ottenendo una più elevata sensibilità dove il recupero massimo di DNA è critico. Il DNA isolato può essere usato nelle successive applicazioni: PCR e Real-Time PCR. Vantaggi:

• Efficienza elevata - ChIP-Adembeads in combinazione con il Blocking Buffers

riducono il background - Nessun problema di sedimentazione.

• Risparmio di tempo e fatica - I passaggi di centrifugazione sono stati sostituiti da una

separazione magnetica rapida, riducendo il tempo dimanipolazione durante il saggio.

- I passaggi indaginosi sono ridotti o completamenteeliminati.

- Nessun pre-clearing. - Cattura e amplificazione diretta: applicabile direttamente

a PCR e Real-time PCR senza il passaggio di eluizione. • Di facile uso: il ChIP-adem-Kit contiene tutti i componenti

necessari per monitorare con successo le interazioni trafattori di trascrizione e istoni con il DNA

• Migliora la sensibilità: più efficiente del sistema con sefarosio.

• Ideale per il trattamento di molti campionicontemporaneamente.

Prodotto Composizione Codice

ChIP-Adembeads Protein A Protein A beads + Blocking Buffer, 40 ChIP ADE-04240

ChIP-Adem-Kit Protein A Complete kit, 25 ChIP ADE-04241

ChIP-Adembeads Protein G Protein G beads + Blocking Buffer, 40 ChIP ADE-04340

ChIP-Adem-Kit Protein G Complete kit, 25 ChIP ADE-04341


Catalogo 2010-11


Cell Sorting: Bio-Adembeads Antibodies Sistema magnetico dedicato alla separazione cellulare (cell sorting) Le Bio-Adembeads di 300nm sono coniugate ad anticorpi (IgG, IgM) AffinityPure diretti contro diverse specie (topo, ratto, coniglio, uomo) Le dimensioni ridotte e costanti e la forma perfettamente sferica delle particelle forniscono uno strumento compatibile con diversi sistemi di analisi quali citofluorimetria a flusso e microscopia Caratteristiche: Biglie coniugate con anticorpi secondari o monoclonali

• Goat Anti-(Mouse, Rat, Rabbit, Human) IgG • Goat Anti-(Mouse, Rat, Rabbit, Human) IgM • Mouse Anti-Biotin • Streptavidin • CD4, CD8 e CD14

Procedura:

Risultati:

Vantaggi:

• Metodo rapido: Cattura tutti i tipi di cellule di specie differenti in meno di 5 minuti • Elevata specificità: Tutti gli anticorpi sono Fc specifici, non c’è cross-reattività • Metodo compatibile con Citofluorimetria e analisi al microscopio

Prodotto Diametro particelle (nm) Volume Codice 2 ml ADE-G05030 10 ml ADE-G05031 Goat anti Mouse IgG 300 50 ml ADE-G05032 2 ml ADE-G05130 10 ml ADE-G05131 Goat anti Rat IgG 300 50 ml ADE-G05132 2 ml ADE-G05230 10 ml ADE-G05231 Goat anti Rabbit IgG 300 50 ml ADE-G05232 2 ml ADE-G05330 10 ml ADE-G05331 Goat anti Human IgG 300 50 ml ADE-G05332 2 ml ADE-G05430 10 ml ADE-G05431 Mouse anti Biotin 300 50 ml ADE-G05432 2 ml ADE-M05030

Goat anti Mouse IgM 300 10 ml ADE-M05031 2 ml ADE-M05130

Goat anti Rat IgM 300 10 ml ADE-M05131 2 ml ADE-M05230

Goat anti Rabbit IgM 300 10 ml ADE-M05231 2 ml ADE-M05330

Goat anti Human IgM 300 10 ml ADE-M05331


Catalogo 2010-11


Cell Sorting: others Bio-Adembeads Streptavidin Le nanoparticelle magnetiche coniugate a Streptavidina forniscono un sistema molto semplice per l’isolamento di molecole biotinilate nella separazione di DNA, purificazione di RNA messaggero, separazione cellulare, purificazione di proteine


0,5 ml ADE-03120

1 ml ADE-03121

5 ml ADE-03122 200

10 ml ADE-03123

0,5 ml ADE-03130

1 ml ADE-03131

5 ml ADE-03132

Bio-Adembeads Streptavidin

300

10 ml ADE-03133


Catalogo 2010-11


Cell Sorting: Viro-Adembeads, virus capture and culture Le Viro-Adembeads sono progettate per la cattura e coltura dei virus, in maniera rapida e semplice. I virus legati alle nanoparticelle magnetiche sono vitali e conservano l’infettività. Il nuovo metodo permette di migliorare l’efficienza d’infezione del virus, concentrandolo e promuovendo il contatto cellula-virus. Le viro-Adembeads facilitano i protocolli comunemente usati per l’infezione virale. Vantaggi:

• Per la cattura e concentrazione dei virus da molteplici campioni biologici. • Semplice e veloce: 30 minuti. • Delicato metodo di separazione dei virus: isola virus vivi e infettivi. • Viro-Adembeads migliora la coltura dei virus: aumentando e accelerando la produzione virale • Sensibilità più elevata paragonata ai metodi convenzionali (centrifugazione) • Riproducibilità ingente rispetto ai metodi convenzionali di centrifugazione.

Prodotto Descrizione Quantità Codice

Viro-AdemBeads Per la cattura e la coltura

dei virus 25 Preparazioni ADE-07010


Catalogo 2010-11


Genomics: Direct mRNA Adembeads purification kit Caratteristiche:

• Consente la purificazione diretta di mRNA a partire da cellule, sangue, tessuto o piante • Permette di lavorare in volumi di eluzione ridotti (10 < x < 50 µl) • Versatile: si adatta alla purificazione di mRNA in piccola o larga scala. • Ridottissima contaminazione da RNA ribosomale • Veloce: il processamento richiede meno di 30 minuti

Procedura:

Risultati:

Vantaggi:

• Rapidità: meno di 20 min • Elevata Resa: rilevazione anche di bassi livelli di mRNA • Elevata purezza • Nessuna degradazione di mRNA • Nessuna contaminazione di DNA • Bassa contaminazione di rRNA • Facilità di esecuzione • Nessuna eluizione richiesta: compatibile con RT-PCR


Direct mRNAdemBeads Purification Kit

30 campioni Fino a 30 milioni di cellule ADE-06021


Catalogo 2010-11


Genomics: others mRNA Adembeads Total mRNA Adembeads purification kit Per la purificazione di mRNA direttamente da cellule, tessuti o sangue intero o per arricchimento da Total RNA.

• Sistema magnetico per la purificazione di mRNA a partire da RNA totale • Pronto all’uso: non è richiesta la rigenerazione delle nanoparticelle magnetiche • Non è richiesta l’eluizione del campione: compatibile con amplificazione direttamente dalle nanoparticelle • Ridottissima contaminazione da RNA ribosomale • Alto grado di purezza, assenza di degradazione, livello di arricchimento dell’mRNA non fornito da altri

protocolli o altre procedure classiche di purificazione su colonna • Volume di eluizione ridotto: compreso tra 10 e 50 µl.


Mini Kit (25 campioni) mRNA da total RNA

(fino a 2,5 mg di total) ADE-06011

Midi Kit (50 campioni) mRNA da total RNA

(fino a 5 mg di total) ADE-06012

mRNAdembeads Purification Kit

Maxi Kit (10 campioni) mRNA da total RNA

(fino a 10 mg di total) ADE-06013

96mRNA Isolation System

• Kit basato su particelle magnetiche in formato piastra da 96 pozzetti che consente la purificazione di mRNA a partire da RNA totale, cellule o sangue

• Perfetto per il processamento simultaneo di molti campioni • Ridotta contaminazione da rRNA • Veloce: il processamento richiede meno di 30 minuti • Utilizzabile direttamente per RT-PCR


96 mRNAdemBeads purification Kit

1X96 campioni Fino a 100.000 cellule ADE-06031

Total RNA Isolation Reagent

• Reagente ottimizzato per estrarre RNA totale di alta qualità privo di contaminazione da DNA • Procedura semplice: reagente unico, pronto all’uso, non necessita di ulteriori trattamenti quali la

digestione con DNAsi • Ottima resa: fino a 100 µg di RNA a partire da 107 cellule


RNA purification Protocollo Fenolo cloroformio in meno di 1 ora 200 ml ADE-10603


Catalogo 2010-11


Genomics: Smart DNA D-N-Ademkit Caratteristiche:

Smart D-N-Adem kit è un innovativo sistema specificamente designato per la cattura del gDNA e l’amplificazione diretta. Il kit è pronto all’uso e contiene tutto l’occorrente per purificare il gDNA, a partire da sangue, PBMC, batteri, cellule e tessuti animali. Le nanoparticelle sono ricoperte da uno specifico polimero, designato per la cattura del DNA tramite interazioni elettrostatiche. Il risultato dell’estrazione è compatibile con PCR diretta e con Real Time PCR, senza la necessità di eluire il campione.

• Reagente ottimizzato per estrarre gDNA di alta qualità in meno di 10 minuti • Procedura semplice e altamente riproducibile • Nessuna eluizione • Nessuna aggiunta di etanolo

Procedura:

Risultati:

Superior DNA recovery: no DNA lost, increased sensivity

A human fragment GAPDH was then amplifed form the samples by Real-Time PCR. Cycle for the Smart D-N-Adem kit samples were 23.10, 27.36, 31.55 and for the column-purified samples, 30.05 and 33.48 and ND (1000, 10 cells respectively).

Vantaggi:

• Cattura e amplificazione diretta senza il passaggio di eluizione • Semplicità di esecuzione e velocità: meno di 10 minuti (incluso il passaggio di lisi) a temperatura

ambiente • Nessuna perdita di DNA: recupero più alto • Sensibilità più elevata rispetto alle altre tecnologie di purificazione • A partire da piccole quantità di campione: 0,1µl di sangue, 10 cellule… • Sistema altamente riproducibile

Prodotto Quantità Codice

Smart D-N-Adem kit for blood Da sangue (1-50µl)

Da PBMC (1-500000) ADE-06111

Smart D-N-Adem kit for cells and tissues Da cellule (1-500000)

Da tessuti animali (1-25 mg) ADE-06121

Smart D-N-Adem kit for bacteria Da 10µl di coltura batterica

Da 1 colonia ADE-06131


Catalogo 2010-11


Supporti e apparati magnetici: Ademtech-Mag SV, MV, HV, MSV, 96 Ademtech offre una vasta gamma di magneti che è stata sviluppata sulla base di una configurazione alta energia: Adem-Mag SV

• Adapted to a small volume of purification • Application: molecular biology • Compact and easy to bring out • Code: ADE-20101

Up to 1,5 ml

Adem-Mag MV

• Adapted to a standard volume of purification • Application: cell sorting, proteins isolation • Code: ADE-20102

Up to 15 ml

Adem-Mag HV

• Adapted to a large volume of purification • Application: large scale isolation • Code: ADE-20103

Up to 50 ml

Adem-Mag MSV

• Adapted to perform up to 12 experiments • Application: molecular biology • Code: ADE-20104

12 position (1,5 or 2 ml)

Adem-Mag 96

• Adapted for 96-well microtitre plates • Applications: molecular biology purification • Code: ADE-20106


SV (Small Volume Magnet) 1 posizione 1,5 o 2 ml ADE-20101

MV (medium Volume Magnet) 1 posizione 15 ml ADE-20102

HV (High Volume Magnet) 1 posizione 50 ml ADE-20103 ADEM-MAG

MSV (Multiple Small Volume) 12 posizioni 1,5 o 2 ml ADE-20104

ADEM-MAG 96 1 x 96 plate magnetic device incluso spaziatore ADE-20106

Spacer for 96 well-plate Spaziatore ADE-20107


Catalogo 2010-11


Sviluppo di Immunoassay Standard-Adembeads: 200, 300 nm

Carboxyl-Adembeads, Amino-Adembeads • Ademtech offre una vasta gamma di particelle magnetiche da usare in immunoassay • Le Standard-Adembeads sono ricoperte da polimeri ad alta densità di COOH o NH2 • Sono appositamente disegnate per immobilizzazione di anticorpi e proteine • La superficie disponibile per il coupling è elevata • Le particelle presentano omogeneità nelle dimensioni sferiche • Le dimensioni ridotte non permettono la sedimentazione: le particelle rimangono in sospensione


1 ml ADE-02110

5 ml ADE-02111 100

10 ml ADE-02112

1 ml ADE-02120

5 ml ADE-02121

10 ml ADE-02122 200

20 ml ADE-02123

1 ml ADE-02130

5 ml ADE-02131

10 ml ADE-02132

COOH

300

20 ml ADE-02133

3 ml ADE-02220

15 ml ADE-02221

30 ml ADE-02222 200

60 ml ADE-02223

3 ml ADE-02230

15 ml ADE-02231

30 ml ADE-02232

NH2

300

60 ml ADE-02233 Masterbeads: 500 nm

Masterbeads carboxylic acid, Masterbeads Streptavidin • Appositamente sviluppate per soddisfare criteri di automazione • Diametro maggiore con differenti funzionalità di superficie • Assicurano procedure di separazione veloci ed efficienti mantenendo alta affinità di legame


1 ml ADE-02150

5 ml ADE-02151

10 ml ADE-02152 COOH 500

20 ml ADE-02153

1 ml ADE-03150

2 ml ADE-03151

5 ml ADE-03152 Streptavidin 500

10 ml ADE-03153

3 ml ADE-02250

15 ml ADE-02251

30 ml ADE-02252 NH2 500

60 ml ADE-02253


Catalogo 2010-11


Reagenti e soluzioni

Reagenti e soluzioni per un’efficiente coniugazione delle proteine

Activation Buffer, EDC, EGDE, Amino-1 Activation Buffer, Amino-2 Activation Buffer, Storage Buffer, Immobilisation Buffer Gamma di tamponi e reagenti pronti all’uso per coupling di proteine specifiche sulla superficie delle nanoparticelle. Sono disponibili tabelle guida per aiutare a scegliere il metodo giusto per l’immobilizzazione delle proteine


Activation Buffer 10X 15 ml ADE-10101

Amino1 Activation Buffer 10X 15 ml ADE-10102

22,5 ml ADE-10103 Amino 2 Activation Buffer 20X

90 ml ADE-10104

Storage Buffer 10X 15 ml ADE-10201

ADEM-BUFFER

Immobilization Buffer 10X 5 ml ADE-10301

15 mg ADE-10401

50 mg ADE-10402

100 mg ADE-10403 EDC

200 mg ADE-10404

3 ml ADE-10501

15 ml ADE-10502

30 ml ADE-10503

Coupling Reagents

EGDE

60 ml ADE-10504 Servizi su commissione

Ademtech, specialista nella produzione di nanoparticelle superparamagnetiche di diverse dimensioni (200nm, 300nm, 500nm) offre la sua esperienza nella coniugazione con le beads. Ogni fase di studio è svolta secondo i criteri del controllo di qualità ISO 9001 allo scopo di assicurare i migliori risultati con la maggior affidabilità e riproducibilità.


Catalogo 2010-11


Transfezione e trasduzione: OZ Biosciences Magnetofection 68

PolyMag CombiMag

Dati sperimentali applicativi su PolyMag e CombiMag PolyMag Neo NeuroMag SilenceMag ViroMag

Dati sperimentali applicativi su ViroMag FluoMag SelfMag Piastre magnetiche Starting Kit Pubblicazioni Magnetofection

Tee technology (lipid based reagents) 87

DreamFect DreamFect Gold EcoTransfect VeroFect FlyFectin Lullaby

Protein Delivery 95

Antibody delivery system Protein delivery system

Related products (gene tools) 99

Bradford Protein Assay DNA Markers

Assay Kits 103

GeneBlaster Beta-Galactosidase X-Gal Staining Kit Short Hairpin RNA (ShRNA)


Catalogo 2010-11


Magnetofection: Principio del metodo Caratteristiche:

Metodo innovativo per migliorare l'efficienza delle trasfezioni. Le nanoparticelle magnetiche, rivestite con molecole cationiche che legano acidi nucleici, reagenti di transfezione o virus da trasportare all'interno della cellula, vi penetrano con alta efficienza tramite un campo magnetico, fornito da una piastra. Per una migliore funzionalità, le Magnetofection sono disponibili come:

• PolyMag, per tutti gli acidi nucleici • CombiMag, per tutti i tipi di vettori (virus e reagenti per trasfezioni) • SilenceMag, per siRNA • ViroMag, per applicazioni virali • FluoMag, particelle fluorescenti

Procedura:

Risultati:

Do not electroshock your cells anymore!

Vantaggi:

• economico (sono necessarie piccole quantità di DNA e di reagente) • efficiente • semplice (consiste in due soli passaggi, vedi disegno esplicativo) • compatibile con Terreni di coltura contenenti siero • atossico (le particelle di per sé non sono tossiche per la cellula e nel giro di 24-72 ore vengono inglobate nei

vacuoli)


Catalogo 2010-11


Magnetofection: PolyMag Caratteristiche: Per tutti gli acidi nucleici e per tutte le condizioni di trasfezione. L’universale e potente formulazione PolyMag permette di ottenere un’efficienza di trasfezione molto elevata. Reagente esclusivo e ottimizzato, composto da nanoparticelle magnetiche rivestite da molecole cationiche, è stato testato con successo con:

• Tutti i tipi di acidi nucleici: DNA "nudo", RNA, siRNA, ODN... • Linee cellulari, cellule primarie e cellule difficili da transfettare. • Cellule adese o in sospensione

Procedura: PolyMag è di facile utilizzo:

• Aggiungere l’acido nucleico a PolyMag • Aggiungere questa miscela alle cellule • Incubare la piastra di coltura sul magnete (15’) • Rimuovere il magnete e procedere come d’abitudine

Risultati:

Vantaggi: Grazie all’efficienza della tecnologia Magnetofection, PolyMag è altamente efficace anche con basse quantità di acidi nucleici. Elevata efficienza di trasfezione può quindi essere ottenuta in tempi brevi e con piccole quantità di materiale, consentendo di risparmiare tempo e denaro. Codice Prodotto Confezione

OZB-PN30100 PolyMag 100 µl

OZB-PN30200 PolyMag 200 µl

OZB-PN31000 PolyMag 1 ml


Catalogo 2010-11


Magnetofection: CombiMag Caratteristiche: Soluzione unica per tutti i vettori. Il reagente CombiMag è stato realizzato per essere di ausilio ai normali reagenti di trasfezione ed è il metodo adatto per implementare l’efficienza dei protocolli standard. CombiMag può essere usato con tutti i tipi di acidi nucleici, complessati con ogni reagente di trasfezione. Tutti i tipi cellulari, anche difficili da trasfettare e cellule primarie, possono essere transfettati con elevata efficienza usando CombiMag. Procedura: CombiMag è molto facile da applicare:

• Preparate il vostro DNA (complessato con il solito reagente di trasfezione) • Aggiungete CombiMag alla miscela di reazione • Aggiungete il complesso ottenuto alle cellule • Incubate la piastra di coltura sul magnete (15’) • Rimuovete il magnete e procedete come d’abitudine.

Risultati:

Primary Chondrocytes (Pig)

Standard Transfection

(reagent F) Magnetofection

CombiMag + reagent

Primary Rat Hippocampal Neuron

Transfection Reagent L + CombiMag

Vantaggi: Sarete in grado di creare il vostro sistema ottimale senza cambiare il protocollo, aumentando significativamente l’efficienza di trasfezione. CombiMag è stato associato a molti reagenti per transfezione esistenti, incluso il nostro DreamFect (lipopoliammine). Codice Prodotto Confezione

OZB-CM20100 CombiMag 100 µl

OZB-CM20200 CombiMag 200 µl

OZB-CM21000 CombiMag 1 ml


Catalogo 2010-11


Magnetofection: Dati sperimentali applicativi Linee cellulari testate con successo:

Cell Line Cell Type Source PolyMag CombiMag

16HBE14o Airway Epithelium Human 181RDB Pancreatic Cells Human n.d. 1,3

143B Osteosarcoma Human n.d. 3 293, HEK-293 293-T, -EBNA

Transfected (embryonic) Kidney Human

3T6 Embryonic fibroblast Mouse A431 Epidermal Carcinoma Human n.d. 3

A549 Non-small cell lung carcinoma Human n.d. 2,3

AM-C6SC8 Kidney Porcine n.d. 3

AR42J Epithelial Rat n.d.

ß-TC Pancreactic Islet ß cells Mouse n.d.

B16F10 Melanoma Mouse B16F10 .9 Melanoma Mouse BEAS-2B Bronchial epithelial cells Human

3

BHK-21 Kidney Hamster BIU-87 Bladder cancer Human n.d.

BTK-143 Osteosarcoma Human 3

C2C12 Myoblast Mouse n.d. 3

C6 Glioma Rat 3

CALU 3 Lung adenocarcinoma (epithelial) Human 3

CHO Epithelial-like (Ovary) Hamster CHO-K1 Epithelial-like (Ovary) Hamster CL7.1 Fibroblast Mouse

3

Colo-205 Colon adenocarcinoma Human n.d. 3

COS-1 Fibroblast (Kidney) Green Monkey COS-7 Fibroblast (Kidney) Green Monkey CRFK Kidney Feline n.d. 3

CT-26 Colon Carcinoma Mouse 2,3

CV-1 Fibroblast-like (Kidney) Monkey DU-145 Prostate Human n.d. 3

ECV-304 Uroepithelium Human n.d. 3

EJ28 Bladder carcinoma Human n.d.

F9 Embryonal teratocarcinoma Mouse n.d.

FaDu Head-&-neck squamous carcinoma Human n.d.

H4IIE Hepatoma Rat n.d. 3

HaCaT Immortalized Keratinocytes Human n.d. HBL-100 Transformed Breast Human n.d. 3

HCT-116 Colon adenocarcinoma Human n.d. 3

HCT-15 Colon adenocarcinoma Human n.d. 3

HeLa Cervical Epithelial Carcinoma Human 2,3

Hep2 Laryngeal Epithelium Human 2,3

Hep 3B Liver Carcinoma Human n.d. 3

HepG2 Hepatoma Human HFF Foreskin Fibroblast Human 3

hOSE Ovarian epithelium Human n.d. 3

HSG Salivary gland epithelium Human n.d. 3

HT1080 Fibrosarcoma Human 2,3

HT-22 Hippocampus Mouse n.d. 3

HT-29 Colon carcinoma Human n.d. 3

HUVEC Endothelial Cells (primary) Human 2,3

Jurkat * Acute-T cell lymphoma Human n.d. 2,3

K-562 * Myelogenous Leukemia Human n.d. KC Embryonic Drosophila n.d.


Catalogo 2010-11


Magnetofection: Dati sperimentali applicativi

Cell Line Cell Type Source PolyMag CombiMag

L6 Muscle cells Rat n.d.

L929 Fibrosarcoma Mouse LNCaP Prostate Carcinoma Human n.d. 3

LoVo Colon adenocarcinoma Human n.d. 3

LS174T Colon adenocarcinoma Human n.d. 3

MEF Embryonic fibroblast Mouse 3

MCF-7 Breast Adenocarcinoma Human MDCK Normal -Kidney Canine MeWo Melanoma Human n.d. 3

mICc12 Intestine Mouse n.d. 3

MRC5 Lung Embryonic Human N2A Neuroblastoma Mouse 2,3

NCI-H82 Small cell lung carcinoma Human n.d.

NIH3T3 Fibroblasts Mouse 2,3

NG108-15 Neuroblastoma - glioma fusion Mouse-Rat n.d. 3

NS20Y Neuroblastoma Mouse 3

OK, OK/NHE1 Kidney Opossum n.d. 3

P815 * Mastocytoma Mouse n.d. 2,3

PC-12 Pheochromocytoma (adrenal) Rat PC3 Prostate carcinoma Human n.d. 3

PT-11 Kidney Fibroblast Bovine n.d. 3

RAW Macrophage (Monocyte) Mouse n.d. 2

Rcho-1 Giant trophoblasts Mouse n.d. 3

SaOS Osteosarcoma Human n.d. 1,3

SH-SY5Y Neuroblastoma Human 2,3

SK-MEL-28 Melanoma Human n.d. 3

SK-MES-1 Lung squamous carcinoma Human n.d.

SKOV-3 Ovarian carcinoma Human n.d. 3

SM10 Placenta Mouse n.d. 3

SW-480 Colon adenocarcinoma Human n.d. 2,3

STC1 Intestinal endocrine Mouse n.d. n.d.

THP-1 * Myelomonocytic Human n.d. 2,3

tsA201 Embryonic kidney Human n.d. 3

U87 Glioma Human 3

U937 * Histiocytic Lymphoma Human V79 Lung Fibroblasts Hamster Vero Kidney Green Monkey 3

VSa13, VSa16 Bone-derived Fish (Sparus aurata) 2

Primary Cells Source PolyMag CombiMag

Airway Epithelium Porcine Human n.d.

Primary Aortic Endothelial Cells (PAEC) Human Bovine

Pig 2

Carotid Artery Smooth Muscle Cells Bovine n.d.

Chondrocytes Pig

Rabbit 2 65-80

Chromaffin Cells Bovine n.d. Cytotrophoblastic cells n.d. Dendritic Human n.d.

Embryonic Fibroblasts (MEF) Mouse n.d. 3

Epicardial mesothelial cells Rat n.d. Diploid Fibroblasts (HDF) Human n.d. 3


Catalogo 2010-11


Magnetofection: Dati sperimentali applicativi

Primary Cells Source PolyMag CombiMag

Fibroblast Human n.d. Fibrochondrocytes Pig 2

Gastric Gland Cells Human

Glial Human

Rat Mouse

Hepatocytes Rat n.d. 3

Hippocampal neurons Mouse

Rat 2,3 10-15

HUVEC Endothelial Cells (primary) Human 2,3

Keratinocytes Human Mouse

n.d.

Mammal Epithelium (HMEC) Human n.d. Nasal Airway Epithelium Human

Neurons Mouse

Rat 2,3

Peripheral Blood Lymphocytes Human Mouse

n.d. 1

Smooth muscle (SMC) Porcine n.d. 2,3

Stroma - Endotrium Human n.d.

Trophoblastic Cells Human n.d. * Suspension Cells 1 Corresponds to successfully transfected in combination with adenovirus 2 Corresponds to successfully transfected by CombiMag in association with another commercial transfection reagent 3 Corresponds to successfully transfected by CombiMag in association with DreamFect™


Catalogo 2010-11


Magnetofection: PolyMag Neo

Caratteristiche:

Questo reagente è stato specificatamente creato per ottenere un’elevata efficienza di trasfezione in combinazione con una superiore espressione del transgene. PolyMag Neo rappresenta l’ultima evoluzione della Magnetofection basata sui reagenti di trasfezione ed è quindi il modo ideale per trasfettare efficacemente e senza tossicità un’ampia gamma di linee cellulari. Procedura: PoliMag Neo è molto facile e rapido da applicare:

• Aggiungete gli acidi nucleici al PoliMag Neo • Aggiungete la miscela alle cellule • Incubate la piastra di coltura sul magnete (15’) • Rimuovete il magnete e procedete come d’abitudine.

Risultati:

Vantaggi: PoliMag Neo è efficace sia su linee cellulari che su cellule primarie; permette di trasfettare hard-t-transfect cells con effetti tossici completamente ridotti.

Codice Prodotto Confezione

OZB-PG60100 PoliMag Neo 100 µl

OZB-PG60200 PoliMag Neo 200 µl

OZB-PG61000 PoliMag Neo 1 ml


Catalogo 2010-11


Magnetofection: NeuroMag Caratteristiche: NeuroMag è il primo sistema magnetico dedicato alla trasfezione di neuroni. E’ perfetto per neuroni primari e può essere utilizzato per linee cellulari e cellule gliali. La trasfezione può essere eseguita con neuroni da 3 a 21 DIV. Grazie a queste proprietà uniche, NeuroMag consente di seguire la maturazione dei neuroni trasfettati a distanza di diversi giorni dall’esperimento di trasfezione. E’ un reagente pronto all’uso che forma complessi non covalenti con gli acidi nucleici attraverso interazioni elettrostatiche e idrofobiche, è completamente biodegradabile, non tossico e non interferisce con i meccanismi cellulari. Procedura: NeuroMag è molto facile e rapido da applicare:

• Aggiungete gli acidi nucleici al NeuroMag • Aggiungete la miscela alle cellule • Incubate la piastra di coltura sul magnete (15’) • Rimuovete il magnete e procedete come d’abitudine.

Risultati:

N2A cells 3 days after transfection with NeuroMag

Comparison of transfection efficiency with other transfection reagents

Vantaggi: NeuroMag è ideale per la trasfezione ad elevata efficienza di neuroni primari; inoltre permette una lunga espressione del transgene (più di 7 giorni) Codice Prodotto Confezione

OZB-NM50200 NeuroMag 200 µl

OZB-NM50500 NeuroMag 500 µl

OZB-NM51000 NeuroMag 1 ml


Catalogo 2010-11


Magnetofection: SilenceMag Caratteristiche: Il più potente trasportatore di siRNA. Semplice, rapido e facile da applicare, SilenceMag non è tossico ed è compatibile con siero. Specificamente designato per siRNA, questo reagente permette lo studio del silenziamento dei geni utilizzando minime quantità di siRNA.

• Elevata efficienza in numerosi tipi cellulari (cellule primarie, linee cellulari e cellule "difficili") • Reagente adatto a tutte le applicazioni per siRNA (co-trasfezioni e silenziamento genico endogeno)

Procedura: SilenceMag è molto facile e rapido da applicare:

• Miscelate siRNA e SilenceMag • Aggiungete la miscela alle cellule • Incubate la piastra di coltura sul magnete (15’) • Rimuovete il magnete e procedete come d’abitudine.

Risultati:

Vantaggi: SilenceMag concentra e introduce grandi quantità di siRNA all’interno delle cellule, conducendo a eccezionali effetti knockdown, anche con dosi molto basse di siRNA, da 10 a 100 volte meno siRNA. Codice Prodotto Confezione

OZB-SM10200 SilenceMag 200 µl

OZB-SM10500 SilenceMag 500 µl

OZB-SM11000 SilenceMag 1 ml

OZB-SM13000 SilenceMag 3 x 1 ml


Catalogo 2010-11


Magnetofection: ViroMag Caratteristiche: Unica soluzione per applicazioni virali. ViroMag una soluzione unica e versatile per molte applicazioni virali, consente di:

• Aumentare l’efficienza di transduzione • Infettare cellule non permissive • Concentrare i virus nelle cellule o nel mezzo di coltura • Sincronizzare l’assorbimento delle cellule (infezioni senza modificazioni dei virus) • Migliorare l’infettività dei virus con basse quantità di vettore • Direzionare la transduzione all’interno di un’area specifica.

ViroMag è il reagente che offre una soluzione a tali applicazioni. Presenta un’efficienza eccezionalmente elevata nel promuovere, controllare e assistere trasduzioni virali. ViroMag e ViroMag R/L

La formulazione ViroMag è applicabile a tutti i virus, mentre ViroMag R/L è una formulazione implementata, specificamente designata per retrovirus e lentivirus. Universalità

ViroMag e ViroMag R/L sono applicabili ad un ampia varietà di linee cellulari e cellule primarie e possono essere combinati con ogni virus (vedi tabella a pagina 29). Risultati:

Vantaggi: L’uso di ViroMag con adenovirus permette un aumento da 500 volte dell’espressione genica, paragonato alle infezioni standard. L’uso di ViroMag R/L con retrovirus o lentivirus permette un miglioramento di 100 volte dell’espressione genica, rispetto alle infezioni standard. Concentrare, promuovere e accelerare l’infezione Titolazioni retrovirali possono essere aumentate da 1000 a 4000 volte. E’ possibile concentrare il virus del morbillo, gli adeno-virus associati, virus non-enveloped e virus enveloped. L’efficienza di trasduzione di adenovirus PEGilati può essere restaurata. Aumentare la capacità virale di infezione Significativi miglioramenti dell’infettività di retrovirus possonoessere ottenuti usando ViroMag e ViroMag R/L.


Catalogo 2010-11


Magnetofection: ViroMag Infettare cellule non permissive L’associazione di virus con ViroMag permette di forzare l’infezione di cellule non permissive.

Sincronizzare la trasduzione Usando i reagenti ViroMag è possibile sincronizzare l’assorbimento dei virus su cellule primarie ed immortalizzate. Non tossico Le particelle di ViroMag e ViroMag R/L non sono tossiche. In piùaccelerando il processo di infezione evitano potenziali effetti dannosidovuti ad una eccessiva permanenza nel mezzo di coltura.

ViroMag R/L, specifico per retrovirus/lentivirus Formulazione implementata di ViroMag, offre più elevata efficienza per tutte le applicazioni virali. Con retrovirus e lentivirus.

Applicazioni per HIV ViroMag R/L è il reagente che permette di aumentare drasticamentel’infezione di cellule CD4+ con HIV.


OZB-VM40100 ViroMag 100 µl

OZB-VM40200 ViroMag 200 µl

OZB-VM41000 ViroMag 1 ml

OZB-RL40100 ViroMag R/L 100 µl

OZB-RL40200 ViroMag R/L 200 µl

OZB-RL41000 ViroMag R/L 1 ml


Catalogo 2010-11


Magnetofection: Dati sperimentali applicativi Linee cellulari testate con successo:

Cell Line Cell Type Source

16HBE Airway epithelium Human

181RDB Pancreatic cells Human

143B Osteosarcoma Human

293, HEK-293 Transformed embryonic kidney Human

A431 Epidermal carcinoma Human

A549 Non-small cell lung carcinoma Human

B16F0, B16F10 Melanoma Mouse

B95a B lymphoblastoid Simian

BHK-21 Kidney Hamster

BT4C Glioma cells Rat

C2C12 Myoblast Mouse

C6 Glioma Rat

CHO, CHO-K1 Epithelial-like (ovary) Hamster

COLO 205 Colon adenocarcinoma Human

COS-1, COS-7 Fibroblast (kidney) Green Monkey

CRFK Kidney Feline

CT-26 Colon carcinoma Mouse

CV-1 Fibroblast-like (kidney) Monkey

D-17P4 Osteosarcoma Canine

DU-145 Prostate Human

ECV-304 Uroepithelium Human

EJ28 Bladder carcinoma Human

H4IIE Hepatoma Rat

HaCaT Immortalized keratinocytes Human

HBL-100 Transformed breast Human

HCT-15 Colon adenocarcinoma Human

HeLa Cervical epithelial carcinoma Human

Hep2 Laryngeal epithelium Human

Hep 3B Liver carcinoma Human

HepG2 Hepatoma Human

hOSE Ovarian epithelium Human

HT1080 Fibrosarcoma Human

HT-22 Hippocampus Mouse

HT-29 Colon carcinoma Human

HUVEC Endothelial cells (primary) Human

Jurkat Acute-T cell lymphoma Human

K-562 Myelogenous leukemia Human

KC Embryonic Drosophila

L6 Muscle cells Rat

L, L929 Fibrosarcoma Mouse

LNCaP Prostate carcinoma Human

LoVo Colon adenocarcinoma Human

LS174T Colon adenocarcinoma Human

MCF-7 Breast Adenocarcinoma Human

MDCK Normal -Kidney Canine

MEF Embryonic fibroblast Mouse

MeWo Melanoma Human

mICc12 Intestine Mouse

N2A Neuroblastoma Mouse

NCI-H82 Small cell lung carcinoma Human

NG108-15 Neuroblastoma - glioma fusion Mouse-Rat

NIH3T3 Fibroblasts Mouse

NS20Y Neuroblastoma Mouse


Catalogo 2010-11


Magnetofection: Dati sperimentali applicativi Cell Line Cell Type Source

P815 Mastocytoma Mouse

PC-12 Pheochromocytoma (adrenal) Rat

PC3 Prostate carcinoma Human

Rcho-1 Giant trophoblasts Mouse

SaOS Osteosarcoma Human

SH-SY5Y Neuroblastoma Human

SK-MEL-28 Melanoma Human

SKOV-3 Ovarian carcinoma Human

SM10 Placenta Mouse

SW-480 Colon adenocarcinoma Human

THP-1 Myelomonocytic Human

tsA201 Embryonic kidney Human

U87 Glioma Human

U937 Histiocytic lymphoma Human

Vero Kidney Green Monkey

Primary Cells Source

Airway epithelium Porcine Human

Primary aortic endothelial cells (PAEC) Human Bovine

Carotid artery smooth muscle cells Bovine

Chondrocytes Pig

Chromaffin cells Bovine

Cytotrophoblastic cells Human Mouse

Embryonic fibroblasts (MEF) Mouse

Epicardial mesothelial cells Rat

Fibroblast Human

Fibrochondrocytes Pig

Gastric myofibroblasts Human

Glial Human Mouse

Rat Hepatocytes Rat

HIppocampal neurons Mouse

Rat HUVEC Human

Keratinocytes Human Mouse

HMEC Human

Nasal airway epithelium Human

Neurons Mouse

Rat

Primary Peripheral Blood Lymphocytes Human Mouse

Smooth Muscle cells (SMC) Porcine

Trophoblastic cells Human

Virus Type Virus name

Adenovirus / Adeno-Associated Virus Ad5 LacZ, Ad5-PEG

Lentivirus / Retrovirus HIV, MuLV, MLV, FIV, SIV

Herpes virus HSV-1

Alpha virus Sindbis virus

Baculovirus

Rhabdovirus VSV

Polyomavirus SV40

Paramyxovirus Measles


Catalogo 2010-11


Magnetofection: FluoMag Caratteristiche: Metodo semplice ed efficiente per trasfettare cellule in coltura e in vivo evidenziandone il percorso: RED FluoMag, particelle magnetiche coniugate con tetrametilrodamina La tetrametilrodamina:

• Non è sensibile a variazioni di pH • È legata covalentemente alle particelle magnetiche: la procedura di marcatura non influisce sull’attività delle

nanoparticelle magnetiche e, di conseguenza, non influenza l’efficienza di trasfezione. Le 4 tipologie standard di prodotto, si trovano ora anche rodaminate:

• FluoMag-P analogo di PolyMag per il trasporto di DNA e acidi Nucleici • FluoMag-S analogo di SilenceMag per RNA Silencing • FluoMag-C analogo di CombiMag • FluoMag-V equivalente a ViroMag per le applicazioni virali

Risultati:

COS7 cells (5x104 cells/well), growing on coverslips, were transfected in 24-well plates with 1 μg/well of pLacZ or pEGFP plasmid and with 1 μl of FluoMag-P per μg of DNA as described in the Magnetofection instruction manual. At several times post-transfection, cells were fixed and observed under a fluorescent microscope equipped with a CCD camera. At 30 minutes and 4 hours-A post transfection, only rhodamine fluorescence was analyzed. In some pictures, nuclei were stained with DAPI (blue).

Vantaggi: Le particelle magnetiche fluorescenti, sono adatte a numerose applicazioni:

• Per doppie marcature e studi di co-localizzazione, utilizzando acidi nucleici marcati con GFP o FITC • Per analisi al FACS, microscopia a fluorescenza e confocale • Per seguire i meccanismi di trasfezione (interazione tra cellule, percorso intracellulare…) • Per FRET (Risonanza a fluorescenza con Trasferimento di Energia) • Come traccianti per lo studio delle vescicole di endocitosi • Per determinare la stabilità dei complessi nei vari “ambienti biologici” • Per analizzare l’associazione di acidi nucleici o reagenti di trasfezione o virus con le particelle magnetiche


OZB-FC10100 FluoMag-C 100 µl

OZB-FP10100 FluoMag-P 100 µl

OZB-FS10100 FluoMag-S 100 µl

OZB-FV10100 FluoMag-V 100 µl


Catalogo 2010-11


Magnetofection: SelfMag Caratteristiche: SelfMag permette di creare il proprio sistema di trasporto magnetico. Questi kit sono stati progettati in modo da consentire l’accoppiamento tra la molecola di interesse e le nanoparticelle magnetiche, in modo da trasportarle all’interno della cellula. Può essere utilizzato per il trasporto di proteine, peptici, oligonucleotidi, droghe o di qualsiasi altra molecola all’interno di cellule vive. La molecola di interesse viene covalentemente accoppiata grazie ai gruppi COOH o NH2 presenti sulla superficie cellulare delle nanoparticelle magnetiche. Queste particelle accoppiate vengono quindi trasportate all’interno della cellula grazie ud un reagente MagFectin. Vengono proposti 2 diversi tipi di Kit: - SelfMag Carboxy Kit contenente gruppi reattivi COOH - SelfMag Amino Kit contenente gruppi reattivi NH2

Risultati:

Vantaggi:

• Disponibile per il trasporto di numerose molecole • Pronto all’uso • Elevata efficienza • Protocollo semplice e dettagliato


OZB-SA10000 SelfMag Amino Kit

OZB- SA11000 SelfMag Amino Beads 1 ml

OZB-SA12000 Buffer Kit A

OZB-SC20000 SelfMag Carboxy Kit

OZB-SC21000 SelfMag Carboxy Beads 1 ml

OZB-SC22000 Buffer Kit C

OZB-SF40000 MagFectin 1 ml


Catalogo 2010-11


Magnetofection: Piastre magnetiche Tre tipologie di piastre magnetiche ottimizzate per efficienti trasfezioni: Super Magnetic Plate:

Nuova piastra, a maggiore intensità magnetica. Studiata per adattarsi a tutti i tipi di supporti per colture cellulari: 6, 12, 24, 96 pozzetti, piastre da 35 mm, T-flasks, etc.

• Code: OZB-MF10000

96-Magnets Plate:

Adatta a tutti i formati ed ideale per le piastre da 96 pozzetti.

• Code: OZB-MF10096

Codice Prodotto

OZB-MF10000 Super Magnetic Plate

OZB-MF10096 96-Magnets Plate

OZB-MF14000 Magnetic Plate for 4 culture dishes


Catalogo 2010-11


Magnetofection: Starting Kit

Codice Prodotto

OZB-KC30200 Magnetofection Starting Kit (OZB-MF10000+OZB-PN30100+OZB-CM20100+OZB-PG60100)

OZB-KC30296 Magnetofection Starting Kit (OZB-MF10096+OZB-PN30100+OZB-CM20100+OZB-PG60100)

OZB-KC30300 Magnetofection Starting Kit (OZB-MF10000+OZB-SM10200)

OZB-KC30396 Magnetofection Starting Kit (OZB-MF10096+OZB-SM10200)

OZB-KC30400 Magnetofection Starting Kit (OZB-MF10000+OZB-PN30100+OZB-CM20100+ OZB-SM10200+OZB-PG60100)

OZB-KC30496 Magnetofection Starting Kit (OZB-MF10096+OZB-PN30100+OZB-CM20100+ OZB-SM10200+OZB-PG60100)

OZB-KC30500 Magnetofection Starting Kit (OZB-MF10000+OZB-VM40200)

OZB-KC30596 Magnetofection Starting Kit (OZB-MF10096+OZB-VM40200)

OZB-KC30600 Magnetofection Starting Kit (OZB-MF10000+OZB-VM40100+OZB-RL40100)

OZB-KC30696 Magnetofection Starting Kit (OZB-MF10096+OZB-VM40100+OZB-RL40100)

OZB-KC30700 Magnetofection Starting Kit (OZB-MF10000+OZB-RL40200)

OZB-KC30796 Magnetofection Starting Kit (OZB-MF10096+OZB-RL40200)

OZB-KC30800 Magnetofection Starting Kit (OZB-MF10000+OZB-NM50200)

OZB-KC30896 Magnetofection Starting Kit (OZB-MF10096+OZB-NM50200)

OZB-KM30200 Magnetofection Starting Kit (OZB-PN30100+OZB-CM20100+OZB-PG60100)

OZB-KM30300 Magnetofection Starting Kit (OZB-PN30100+OZB-SM10200+OZB-PG60100)

OZB-KM30350 Magnetofection Starting Kit (OZB-CM20100+OZB-SM10200)

OZB-KM30400 Magnetofection Starting Kit (OZB-PN30100+OZB-CM20100+OZB-SM10200+OZB-PG60100)

OZB-KM30500 Magnetofection Starting Kit (OZB-VM40100+OZB-RL40100)


Catalogo 2010-11


Magnetofection: Pubblicazioni Generality / Technology / Method

Author Title References

Kim EA & al. Phosphorylation and transactivation of Pax6 by homeodomain-interacting protein kinase 2 J Biol Chem 2006 Jan.; 10.1074/jbc.M507227200

Ferrari S. & al. Barriers to and new approaches for gene therapy and gene delivery in cystic fibrosis

Advanced Drug Delivery Reviews 2005 Dec. ; Vol54, Issue 11, p1373-1393

Bonetta L. The inside scoop—evaluating gene delivery methods Nature Methods 2005 Nov. ; 2, 875-883

Bystrzejewski M. & al. Arc plasma route to carbon-encapsulated magnetic nanoparticles for biomedical applications

Sensors and Actuators B: Chemical 2005 Aug; Volume 109, Issue 1, P 81-85

Gupta AK & al. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications

Biomaterials 2006 June ; Volume 26, Issue 18, p3995-4021

Neuberger T. & al. Superparamagnetic nanoparticles for biomedical applications: Possibilities and limitations of a new drug delivery system

J Magn Magn Mat. 2005 May; Volume 293, Issue 1, p483-496

Hengge UR & al. Progress and prospects of skin gene therapy: a ten year history

Clinics in Dermatology 2005 Jan. ; Vol 23, Issue 1, p107-114

Conwell CC & al. Recent Advances in Non-viral Gene Delivery Advances in Genetics 2005 ; Volume 53, p1-18

Davies JC & al. Airway Gene Therapy Advances in Genetics 2005 ; Volume 54, p291-314

Mehier-Humbert JS & al. Physical methods for gene transfer: Improving the kinetics of gene delivery into cells.

Advanced Drug Delivery Reviews 2005 ; 57(5) :733-753

Griesenbach U. & al Advances in cystic fibrosis gene therapy Curr Opin Pulm Med. 2004 Nov;10(6):542-546

Wagner E. & al. Targeted nucleic acid delivery into tumors: new avenues for cancer therapy

Biomedecine & Pharmacotherapy 2004 Apr. ; Vol 58, Issue 3, p152-161

Gould P. & al. Nanoparticles probe biosystems. Materials Today 2004 Feb ; 36 :43 :00

Berry CC. & al. The influence of transferrin stabilised magnetic nanoparticles on human dermal fibroblasts in culture.

Intl J Pharma 2004 ; Jan 269(1) ; 211-225

Plank C. & al. Enhancing and targeting nucleic acid delivery by magnetic force.

Expert Opin. Biol. Ther. 2003 Aug; 3(5): 745-758.

Krotz F. & al. Magnetofection--a highly efficient tool for antisense oligonucleotide delivery in vitro and in vivo. Mol. Ther. 2003 May; 7 (5 Pt 1): 700-710.

Plank C. & al. The magnetofection method: using magnetic force to enhance gene delivery.

Biol. Chem. 2003 May; 384 (5): 737-747.

Plank C. & al. Magnetofection: enhancing and targeting gene delivery with superparamagnetic nanoparticles and magnetic fields. J. Liposome Research 2003 Feb; 13 (1): 29-32.

Schmidt-Wolf GD & al. Non-viral and hybrid vectors in human gene therapy: an update

Trends in Mo Med 2003 Feb. ; Vol9, Issue 2, p67-72

Scherer F & al. Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo.

Gene Ther. 2002 Jan; 9(2): 102-109.

PolyMag reagent

Author Title References Notes

Doshida M. & al. Raloxifene increases proliferation and upregulates telomerase activity in HUVEC

JBC, 23 June 2006-07-18 DOI:10.1074/jbc.M513251200 siRNA delivery in HUVEC

Sapet C. & al. Thrombin-induced endothelial microparticle generation: Identification of a novel pathway involving ROCK-II activation by caspase-2

Blood, May 2006, DOI:10.1182/blood-2006-04-014175

Transfection of HMEC-1 cells

Recklies AD & al. Inflammatory cytokines induce production of chitinase-3-like protein 1 by articular chondrocytes.

J Biol Chem 2005 Oct . M5:02325 Successful transfection of Chondrocytes

Fransen M. & al. Analysis of human Pex19p's domain structure by pentapeptide scanning mutagenesis.

J Mol Biol. 2005 Mar 11;346(5):1275-86.

Efficient transfection of Fibroblasts with PolyMag reagent

Schillinger U & al. Advances in Magnetofection – Magnetically guided nucleic acid delivery.

J Magn Magn Mat. 2005; 293:501-508

CombiMag reagent

Author Title References Notes

Mizutani T. & al. Mechanisms of establishment of persistent SARS-CoV-infected cells

BBRC, 2006 June, 347 (2006) 261-265

CombiMag + VeroFect transfection reagent is the best system for transfection of Vero-E6 cells.

Basile JR & al. Semaphorin 4D provides a link between axon guidance and tumor-induced angiogenesis

PNAS, 2006 June, vol. 106 No. 24, 9017-9022

High efficiency of CombiMag for HNSCC cells transfection with shRNA

Kaneko M. & al. Activity of a novel PDGF β-receptor enhancer during the cell cylcle and upon differentiation of neuroblastoma

Exp Cell Res, April 2006 Doi:10.1016/j.yexcr.2006.03.05

BE2 neuroblastoma cells transfection with Combimag associated to another reagent

Kaur S. & al. Robo4 signaling in endothelial cells imply attraction guidance mechanisms

J. Biol. Chem, 10.1074/jbc.M508853200

Transfection of PAEC with CombiMag associated to another reagent


Catalogo 2010-11


Magnetofection: Pubblicazioni

Basile JR & al.

Semaphorin 4D/plexin-B1 induces endothelial cell migration through the activation of PYK2, Src, and the phosphatidylinositol 3-kinase-Akt pathway.

Mol Cell Bio. 2005 Aug; 25(16):6889-98..

76% of transfection efficiency on PAEC cells with CombiMag reagent

Chudotvorova I. & al. Early expression of KCC2 in rat hippocampal cultures augments expression of functional GABA synapses.

J Physiol. 2005 Aug 1; 566 (Pt3):671-9.

3 times enhancement in primary hippocampal neurons

Uchida Y. & al. Semaphorin3A signalling is mediated via sequential Cdk5 and GSK3β phosphorylation of CRMP2…

Genes to Cells 2005 Feb, vol. 10, p.165

Transfection of siRNA in primary culture of rat embryonic DRG (E14) performed with CombiMag associated to antoher transfection reagent

Mizutani T. & al. Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells

FEMS Immunol Med Microbiol, 2005

Transfection of siRNA in Vero cells using CombiMag associated to antoher transfection reagent

Gersting SW & al. Gene delivery to respiratory epithelial cells by magnetofection.

J Gene Med. 2004 Aug; 6(8): 913-922.

Epithelial cells transfected with CombiMag

Huth S. & al. Insights into the mechanism of magnetofection using PEI-based magnetofectins for gene transfer.

J Gene Med. 2004 Aug; 6(8): 923-936.

Krotz F. & al. Magnetofection potentiates gene delivery to cultured endothelial cells.

J. Vasc. Res. 2003 Sep-Oct; 40(5): 425-434.

Transfection of endothelial cells with CombiMag

Xiang JJ & al. IONP-PLL: a novel non-viral vector for efficient gene delivery.

J Gene Med. 2003 Sep;5(9):803-817.

In vivo applications (lung, brain, spleen, kidney)

Hirao K. & al. Targeted gene delivery to human osteosarcoma cells with magnetic cationic liposomes under a magnetic field.

Int J Oncol. 2003 May;22(5):1065-1071

Transfection of osteosarcoma cells

SilenceMag reagent

Autor Title References Notes

Sapet C. & al. Thrombin-induced endothelial microparticle generation: Identification of a novel pathway involving ROCK-II activation by caspase-2

Blood, May 2006, DOI:10.1182/blood-2006-04-014175

Transfection siRNA delivery in HMEC-1 cells

Smith C. Sharpening the tools of RNA interference Nature Methods, June 2006, 475:486 State-of-the-art for siRNA delivery

McCaig & al. The role of matrix metalloproteinase (MMP)-7 in redefining the gastric micro-environment in response to Helicobacter pylori

Gastroenterology, May 2006 Transfection of siRNA into primary gastric myofibroblasts with SilenceMag

ViroMag & ViroMag R/L reagents (and other magnetic particles used with viruses)

Autor Title References Virus type / Application

Kadota SI & al. Enhancing of measles virus infection by magnetofection.

J Virol Methods. 2005 May 16

Mok H. & al. Evaluation of polyethylene glycol modification of first-generation and helper-dependent adenoviral vectors to reduce innate immune responses.

Mol Ther. 2005 Jan;11(1):66-79.

Chan L. & al. Conjugation of lentivirus to paramagnetic particles via nonviral proteins allows efficient concentration and infection of primary acute myeloid leukemia cells.

J Virol 2005; 79(20):13190-4 Lentivirus & primary acute myeloid leukemia cells

Morishita N. & al. Magnetic nanoparticles with surface modification enhanced gene delivery of HVJ-E vector.

Biochemical and Biophysical Research Communications 2005; 334(4):1121-1126

Campos SK & al. Avidin-based targeting and purification of a protein IX-modified, metabolically biotinylated adenoviral vector.

Mol Ther 2004 Jun;9(6):942-54. Adenovirus

Raty JK & al. Enhanced gene delivery by avidin-displaying baculovirus. Mol Ther 2004 Feb;9(2):282-91 Baculovirus

Sato H & al. Virus concentration using polyethyleleimine-conjugated magnetic beads for improving the sensitivity of nucleic acid amplification tests

J Virol Methods. 2003 Dec; 114:11-19

Concentration of viruses

Tai MF & al. Generation of magnetic retroviral vectors with magnetic nanoparticles.

Rev. Adv. Mater. Sci. 5 (2003) 319-323.

Pandori M. & al. Adenovirus-microbead conjugates possess enhanced infectivity: a new strategy for localized gene delivery.

Virology 2002 Aug 1; 299(2):204-12

Adenovirus

Mah C. & al. Improved method of recombinant AAV2 delivery for systemic targeted gene therapy. Mol Ther. 2002 Jul; 6(1):106-12 AAV

Hughes C. & al. Streptavidin paramagnetic particles provide a choice of three affinity-based capture and magnetic concentration strategies for retroviral vectors.

Mol Ther. 2001 Apr;3(4):623-30


Catalogo 2010-11


Tee technology Caratteristiche:

Tee Technology: la nuova generazione di lipopoliammine. Per risparmiare tempo, materiale ed euro La Tecnologia Tee (Triggered Endosomal Escape) combina e sfrutta le proprietà dei lipidi cationici e dei polimeri, per realizzare un trasporto estremamente efficiente, di DNA all’interno delle cellule. A questa tecnologia si possono ascrivere prodotti diversi con punti di forza diversi:

• DreamFect • EcoTransfect • VeroFect

Procedura:

Due soli semplici passaggi:

• Miscelare DNA e reagente • Aggiungere alle cellule

Vantaggi:

• Compattamento del DNA all’interno di nanoparticelle, efficientemente internalizzate dalle cellule • Protezione degli acidi nucleici contro la degradazione delle nucleasi • Efficiente destabilizzazione della membrana e trasporto all’interno della cellula • Altamente efficiente anche con basse quantità di acidi nucleici


Catalogo 2010-11


Tee technology: DreamFect Caratteristiche: DreamFect: l’universale E’ adatto a tutti i tipi di vettori: DNA plasmidico, mRNA, siRNA, oligonucleotidi, etc. Può essere usato per tutte le applicazioni: trasfezioni stabili e transienti, co-trasfezioni, high throughput screening…

I complessi formati da DNA e DreamFect posseggono un eccezionale capacità di destabilizzare la membrana cellulare, permettendo il trasporto di importanti quantità di DNA nel citosol. La trasfezione è altamente efficiente e riproducibile, anche con piccole quantità di acidi nucleici. Risultati:

Vantaggi:

• Universale (per linee cellulari e cellule primarie) • Polivalente (per diversi tipi di acidi nucleici) • Estremamente efficiente • Semplice, pronto all’uso e rapido • Non tossico • Compatibile con terreni di coltura contenenti siero • Economico


OZB-DF40500 DreamFect 0,5 ml

OZB-DF41000 DreamFect 1 ml

OZB-DF45000 DreamFect 5 x 1 ml


Catalogo 2010-11


Tee technology: DreamFect Gold

Caratteristiche: DreamFect Gold: reagenti per trasfezione di ultima generazione Permette di trasfettare tutti i tipi di acidi nucleici con una efficienza davvero alta. Grazie alla sua formulazione DremFectTM Gold offre una grande quantità di acidi nucleici che consentono un elevata espressione proteica, superiore rispetto ad altri reagenti di trasfezione.

E’ completamente biodegradabile e non interferisce con i meccanismi cellulari. Conseguentemente è mantenuta un’alta vitalità cellulare in ogni esperimento e qualsiasi potenziale effetto secondario è evitato. Vantaggi:

• Livelli di espressione proteica superiori ad altri reagenti di trafezione • Elevata efficienza di trasfezione • Polivalente (per tutti i tipi di acidi nucleici) • Biodegradabile • Semplice, pronto all’uso e rapido • Compatibile con terreni di coltura contenenti siero


OZB-DG80500 DreamFect Gold 0,5 ml

OZB-DG81000 DreamFect Gold 1 ml

OZB-DG85000 DreamFect Gold 5 x 1 ml


Catalogo 2010-11


Tee technology: EcoTransfect Caratteristiche: EcoTransfect: economico Reagente dedicato alle trasfezioni delle linee cellulari più comuni, per esperimenti quotidiani. E’ la soluzione ideale per analizzare rapidamente l’attività biologica degli acidi nucleici, per eseguire trasfezioni di routine a basso costo.

Può essere usato per tutti gli esperimenti di routine con DNA plasmidico, DNA lineare, frammenti da PCR attivati trascrizionalmente, mRNA, oligonucleotidi non-senso. Risultati:

Vantaggi:

• Estremamente efficiente • Semplice, pronto all’uso e rapido • Non tossico • Compatibile con terreni di coltura contenenti siero • Economico


OZB-ET10500 EcoTransfect 0,5 ml

OZB-ET11000 EcoTransfect 1 ml

OZB-ET13000 EcoTransfect 3 x 1 ml


Catalogo 2010-11


Tee technology: VeroFect Caratteristiche: VeroFect: specifico per Vero Potente reagente, dedicato alla trasfezione delle cellule Vero; specificamente designato per ottenere trasfezioni altamente efficienti e riproducibili di cellule Vero. Risultati:

Vantaggi:

• Altamente efficiente • Semplice, pronto all’uso, rapido • Non tossico • Compatibile con terreni di coltura contenenti siero • Economico • Versatile per trasfezioni stabili e transienti • Sviluppato specificamente per le cellule Vero, è anche performante su altri tipi cellulari


OZB-VF60250 VeroFect 0,25 ml

OZB-VF60500 VeroFect 0,5 ml

OZB-VF61000 VeroFect 1 ml

OZB-VF65000 VeroFect 5 ml


Catalogo 2010-11


Tee technology: FlyFectin Caratteristiche: FlyFectin: let’s fly together FlyFectin è un reagente per trasfezione molto efficiente, specificamente designato per cellule da insetto. La sua formulazione innovativa è:

• Altamente efficiente, con un ‘eccellente riproducibilità, anche con limitate quantità di acidi nucleici • Polivalente (per i diversi tipi di acidi nucleici) • Ideale per la produzione di proteine ricombinanti con il sistema d’espressione Baculovirus. • Semplice, pronto all’uso e rapido • Compatibile con siero • Non tossico: formulazione altamente efficace a basse concentrazioni, evitando anche eventuali effetti tossici

secondari Una soluzione unica Il reagente FlyFectin è costituito da una soluzione acquosa di lipidi cationici e neutri ed è stato sviluppato esclusivamente per transfettare le cellule d’insetto. Il reagente è perfetto per produrre elevati livelli di proteine ricombinanti in associazione con un sistema d’espressione con Baculovirus. Efficienza e riproducibilità I complessi formati da DNA e FlyFectin, hanno un eccezionale capacità di destabilizzare la membrana cellulare, permettendo il trasporto di importanti quantità di DNA nel citosol. Universale e polivalente FlyFectin è adatto al trasporto di DNA, mRNA, siRNA o oligonucleotidi ed è stato testato con successo su numerose cellule da insetto. Questo reagente, può essere usato per tutte le applicazioni (Trasfezioni stabili o transienti, co-trasfezioni, …) ed è compatibile con terreni contenenti siero. Procedura:

Risultati:

Vantaggi:

• Economico • Aumenta la resa nella produzione della proteina d’interesse • Risparmia materiale: meno DNA e consumabili • Risparmia tempo: sistema veloce con risultati migliori • Stabile per almeno un anno se conservato a +4°C • Trasportato a temperatura ambiente


OZB-FF50500 FlyFectin 0,5 ml

OZB-FF51000 FlyFectin 1 ml

OZB-FF55000 FlyFectin 5 x 1 ml


Catalogo 2010-11


Tee technology: Lullaby® Caratteristiche: Lullaby®: una dolce canzone per silenziare i vostri geni L’RNA interference è una tecnica potente per abbattere l’espressione genica in cellule e organismi. Costituisce un metodo per studiare le funzioni dei geni e contemporaneamente, un promettente approccio per nuovi trattamenti terapeutici. Lullaby® è in grado di trasportare i siRNA in un’ampia varietà di cellule, con un’efficienza elevatissima, conducendo ad eccezionali effetti knockdown, a basse concentrazioni di siRNA. Grazie alle sue esclusive proprietà, Lullaby® è un reagente per il silenziamento genico robusto e delicato contemporaneamente. Silenziamento genico affidabile ed altamente riproducibile Basato su una tecnologia innovativa, questo nuovo reagente realizza un trasporto di siRNA all’interno delle cellule estremamente efficiente:

• Protegge i siRNA dalla degradazione extracellulare • Concorre all’introduzione del siRNA, destabilizzando la membrana cellulare • Permette l’ingresso di quantità importanti di siRNA nel citosol • Permette elevati livelli di silenziamento genico in molteplici condizioni cellulari e colturali. E’ stato

testato con successo su molteplici linee cellulari, raggiungendo fino al 90% di silenziamento genico. • Procedura semplice e rapida: il reagente è pronto all’uso. L’esperimento di silenziamento genico si svolge in

3 semplici passaggi. Il test può essere eseguito dopo una breve incubazione: i primi significativi effetti del knockdown genico possono essere osservati e analizzati già 24h dopo la trasfezione.

• Flessibile e adatto a tutte le condizioni di coltura: Lullaby® è compatibile con antibiotici e con siero; lavora inoltre su un ampio spettro di confluenza cellulare (tra il 20 e il 90%)

Risultati:


Catalogo 2010-11


Tee technology: Lullaby®

Vantaggi:

• Altamente riproducibile, anche con piccole quantità di siRNA: è stato dimostrato che elevate concentrazioni di siRNA (›100nM) possono causare aspecificità.

• Le concentrazioni ideali per la trasfezione sono: 1nM‹siRNA‹20nM • L’efficienza di Lullaby® è stata verificata a 0,1nM ed è ottimale nell’intervallo 5nM‹siRNA‹10nM, evitando

così effetti non specifici Codice Prodotto Confezione

OZB-LL70500 Lullaby® – siRNA Transfection reagent 0,5 ml

OZB-LL71000 Lullaby® – siRNA Transfection reagent 1 ml

OZB-LL73000 Lullaby® – siRNA Transfection reagent 3 x 1 ml


Catalogo 2010-11


Protein Delivery: Antibody delivery system Ab-DeliverIN™ è il primo reagente dedicato al trasporto intracellulare di anticorpi. Questa formulazione basata sui lipidi, è la prima, compatibile con siero, a trasportare anticorpi funzionali in cellule viventi. Proprio per le sue specifiche proprietà, Ab-DeliverIN™ forma complessi non-covalenti con gli anticorpi, attraverso interazioni elettrostatiche ed idrofobiche. Conseguentemente, non c’è la necessità di creare un legame covalente (chimico o genetico). Ab-DeliverIN™ apre nuovi orizzonti investigativi per chiarire i complessi meccanismi molecolari, o per studiare nuove potenziali terapie. Ad esempio, il trasporto intracellulare di anticorpi bloccanti , potrebbe inibire e/o neutralizzare la funzione delle proteine. Vantaggi:

• Trasporto di anticorpi funzionalmente attivi • Elevata efficienza in molte cellule primarie e linee cellulari • Compatibile con siero • Biodegradabile e non citotossico • Protocollo Semplice e pronto all’uso

Localizzazione intracellulare di anticorpi funzionalmente attivi:

Gli anticorpi trasportati mantengono la loro struttura e funzione. Questa caratteristica fa di Ab-DeliverIN™ un reagente esclusivo che permette all’anticorpo di raggiungere il suo obiettivo intracellulare. Ora è possibile bloccare la funzione di una proteina o mappare l’interno della cellula.

Trasfezione di anticorpi estremamente efficiente:

E’ possibile ottenere un grande numero di cellule positive, contenenti l’anticorpo. Paragonato ad altri sistemi, Ab-DeliverIN, mostra un’elevata efficienza di trasporto all’interno di cellule viventi. Il sistema è stato testato con successo su un’ampia gamma di linee cellulari e colture primarie, inclusi neuroni e cellule gliali. Siero-compatibile:

Ab-DeliverIN™ è compatibile anche con mezzi colturali contenenti siero. gli esperimenti possono essere condotti direttamente e rapidamente in vitro e in vivo. L’efficienza più elevata può essere raggiunta in meno di 5 ore.

Biodegradabile e non citotossico:

Ab-DeliverIN™ è completamente biodegradabile e non interferisce con i meccanismi cellulari. Grazie alla sua specifica formulazione, Ab-DeliverIN™ viene rapidamente degradato all’interno delle cellule, mantenendo un’elevata sopravvivenza cellulare in ogni esperimento. Semplice e rapido:

Ab-DeliverIN™ è un metodo rapido e semplice, utile per lo studio della funzione degli anticorpi. Ab-DeliverIN™ è un reagente pronto all’uso, ed il trasporto dell’anticorpo si realizza in 3 passaggi:

1. Miscelare Ab-DeliverIN™ e anticorpo 2. Incubare 10 min a temperature ambiente 3. Aggiungere alle cellule e incubare in condizioni standard


Catalogo 2010-11


Protein Delivery: Antibody delivery system Codice Prodotto Volume Nr. di esperimenti

OZB-AI20100 Ab-DeliverIn™; 100 µl 100 µL Up to 100



OZB-AI21000 Ab-DeliverIn™; 1 ml 1 mL Up to 1000 Conservazione:

Dal momento del ricevimento e per un uso a lungo termine, conservare a + 4°C. I kit Ab-DeliverIN™ sono stabili per almeno un anno, se conservati alla temperatura consigliata. Condizioni di trasporto:

I kit Ab-DeliverIN™ sono trasportati a temperatura ambiente.

Example of antibody delivery efficiency in various cell lines and primary cells Cell Line Cell Type Source % of positive cells

3T6 Embryonic fibroblasts Mouse >50%

A549 Non-small cell lung carcinoma Human 50-80%

B16-F10 Melanoma Mouse >50%

BEAS-2B Bronchial epithelial cells Human 80%

BHK21 Fibroblasts (Kidney) Hamster 80-90%

CHO-K1 Epithelial-like (Ovary) Hamster 50-80%

COS-1, COS-7 Fibroblasts (Kidney) Green Monkey 50-70%

HaCaT Keratinocytes Human 50-80%

HEK-293 Transformed Embryonic (Kidney) Human 80-100%

HeLa Cervical Epithelial Carcinoma Human 50-60%

L929 Fibrosarcoma Mouse 80-90%

K562 Myelogenous leukemia Human 10-50%

MDCK Epithelial (Kidney) Canine 10-50%

N2A Neuroblastoma Mouse 60-80%

NIH3T3 Fibroblasts Mouse 50-75%

Raw264.7 Monocytes/macrophages Mouse 90%

U87 Glioblastoma Human >50%

Vero 10A1 Epithelial (Kidney) Monkey >50%

Primary cells

Neurons Rat >50%

Glial cells Rat >50%


Catalogo 2010-11


Protein Delivery: Protein delivery system Pro-DeliverIN™ è un reagente innovativo, che permette il trasporto intracellulare di proteine biologicamente attive. Questa formulazione basata sui lipidi, è la prima, compatibile con siero, a trasportare proteine funzionali in cellule viventi. Proprio per le sue specifiche proprietà, Pro-DeliverIN™ è in grado di catturare proteine attraverso interazioni elettrostatiche ed idrofobiche. Conseguentemente, non c’è la necessità di creare un legame covalente (chimico o genetico). Pro-DeliverIN™ permette di approfondire la funzione di specifiche proteine, la localizzazione proteica, facendo chiarezza su nuovi meccanismi molecolari… Vantaggi:

• Trasporto di proteine funzionalmente attive • Elevata efficienza in molte cellule primarie e linee cellulari • Compatibile con siero • Biodegradabile e non citotossico • Protocollo Semplice e pronto all’uso

Trasporto di proteine funzionalmente attive:

Pro-DeliverIN™ permette il trasporto di proteine funzionalmente attive, rendendo possibile lo studio di complessi meccanismi molecolari, quali l’apoptosi, la divisione cellulare…

Trasfezione di proteine estremamente efficiente:

Le proteine vengono trasportate efficientemente nel citoplasma di un gran numero di cellule viventi. Numerose proteine diverse possono essere trasportate all’interno delle cellule, ad esempio la Ficoeritrina (proteina fluorescente), l’Albumina sierica, la ß-Galattosidasi, la Caspasi... Trasfezione di proteine estremamente efficiente:

Le proteine vengono trasportate efficientemente nel citoplasma di un gran numero di cellule viventi. Numerose proteine diverse possono essere trasportate all’interno delle cellule, ad esempio la Ficoeritrina (proteina fluorescente), l’Albumina sierica, la ß-Galattosidasi, la Caspasi... Siero-compatibile:

Pro-DeliverIN™ è adatto anche a colture cellulari contenenti siero. In questo modo, non è richiesto alcun cambiamento di mezzo colturale. E’ possibile realizzare direttamente tutti gli esperimenti in vitro in vivo ottenendo il trasporto di una cospicua quantità di proteina.

Biodegradabile e non citotossico:

Pro-DeliverIN™ è completamente biodegradabile e non interferisce con I meccanismi cellulari. Grazie alla sua specifica formulazione, Pro-DeliverIN™ viene rapidamente degradato all’interno delle cellule, mantenendo un’elevata sopravvivenza cellulare in ogni esperimento. Universale e Versatile:

Pro-DeliverIN™ è stato testato con successo su un’ampia varietà di linee cellulari e di cellule primarie, neuroni compresi. Pro-DeliverIN™ permette il trasporto di sequenze di aminoacidi all’interno delle cellule (peptidi, proteine, anticorpi….)


Catalogo 2010-11


Protein Delivery: Protein delivery system Semplice e rapido:

Pro-DeliverIN™ è un metodo rapido e semplice, utile per lo studio della funzione delle proteine. Pro-DeliverIN™ è un reagente pronto all’uso, ed il trasporto proteico si realizza in 3 passaggi:

1. Miscelare Pro-DeliverIN™ e proteine 2. Incubare 10 min a temperature ambiente 3. Aggiungere alle cellule e incubare in condizioni standard

Codice Prodotto Volume Nr. di esperimenti

OZB-PI10100 Pro-DeliverIn™; 100 µl 100 µL Up to 100



OZB-PI11000 Pro-DeliverIn™; 1 ml 1 mL Up to 1000 Conservazione:

Dal momento del ricevimento e per un uso a lungo termine, conservare a + 4°C. I kit Pro-DeliverIN™ sono stabili per almeno un anno, se conservati alla temperatura consigliata. Condizioni di trasporto:

I kit Pro-DeliverIN™ sono trasportati a temperatura ambiente.


Catalogo 2010-11


Related products: Bradford Protein Assay Caratteristiche: Bradford Protein Assay Kit (B-Pak) Procedura semplice e rapida per determinare la concentrazione delle proteine in soluzione. Il kit si basa sul legame tra il colorante Coomassie Brilliant Blue G-250 e la proteina: in particolare, il colorante si lega ai residui aminoacidici basici ed aromatici. Il colorante esiste in 3 forme:

• Cationica (rosso) • Neutra (verde) • Anionica (blu)

In condizioni acide, il colorante si trova prevalentemente nella forma cationica protonata (rosso, Amax=595nm). La concentrazione della proteina viene quantificata nella forma non protonata blu, rilevata a 595nm. Il B-Pak offre Bradford Reagent 1X e 2 set di standard BSA. Queste convenienti quantità, permettono di eseguire sufficienti saggi per la maggior parte delle applicazioni:

Protein Concentration

Low: 0,5 – 50 μg/ml High: 50 – 1500 μg/ml

96-well plate 5000 assays 3570 assays

1 ml cuvette 1000 assays 595 assays

Reagenti Compatibili

Acetone 10% Guanidine-HCl, 2M Sodium acetate pH4.8, 0.2M

Acetonitrile 10% HCl, 0.1M Sodium azide, 0.5%

Ammonium sulfate, 1M HEPES, 0.1M Sodium bicarbonate, 0.2M

Ampholytes, 0.5% Imidazole, 0.2M Sodium carbonate, 0.1M

ASB-14, 0.025% Magnesium chloride, 1M Sodium chloride, 2.5M

Ascorbic Acid, 50mM MES, 0.1M Sodium citrate, pH4.8 or 6.4, 0.2M

Bis-Tris, pH6.5, 0.2M Methanol, 10% Sodium hydroxide, 0.1M

β-mercaptoethanol, 1M MOPS, 0.1M Sodium phosphate, 0.5M

Calcium chloride, 40mM NAD, 2mM Sucrose 10%

CHAPS, 10% NP-40, 0.25% TBP, 5mM

CHAPSO, 10% Octyl b -glucoside, 0.5% TCEP, 20mM

Deoxycholic acid, 0.2% Octyl b -thioglucopyranoside, 1% Thio-urea, 1M

DMSO, 5% Phenol Red, 0.5 mg/ml Tricine, pH8, 50mM

DTE, 10mM PIPES, 0.2M Triethanolamine, pH7.8, 50mM

DTT, 5mM PMSF, 2mM Tris, 1M

EDTA/EGTA, 0.2M Potassium chloride, 2M Tris-glycine

Ethanol, 10% Potassium phosphate, 0.5M Triton X-100, 0.05%

Glucose, 20% RIPA lysis buffer, 1/40 dilution Tween 20, 0.01%

Glycerol, 5% SB 3-10, 0.1% Urea 4M

Glycine, 0.1M SDS, 0.025%


Catalogo 2010-11


Related products: Bradford Protein Assay Risultati:

Vantaggi: Kit estremamente accurato che permette di risparmiare tempo e reagenti:

• Test semplice, pronto all’uso, che si attua in pochi minuti: non sono richiesti né diluizioni, né filtrazioni, né calcoli.

• Il kit viene fornito con protocollo adattabile a tutte le condizioni sperimentali (micro e macroassay, concentrazione di tutte le proteine…). Un solo kit per tutte le determinazioni.

• Determinazione precisa della concentrazione della proteina, in presenza di detergente (0,1%): kit utilizzabile per esperimenti di trasfezione, in combinazione con saggi di “gene reporter”.

• Linearità di risposta superiore, con tutte le concentrazioni di proteina (da 0,5 a 1500 µg/ml).

Codice Prodotto Confezione Numero di assays

Low concentration High concentration

OZB-BA00100 Bradford-Protein Assay Kit, B-Pak 1X Bradford reagent, 500 ml

2 sets of 7 BSA standards 5000 3570

OZB-BA00050 1X Bradford reagent 1X Bradford reagent, 500 ml 5000 3570 OZB-BA00070 Set of standards 2 sets of 7 BSA standards - -


Catalogo 2010-11


Related products: DNA Markers

Caratteristiche: • All markers are produced from certified high quality plasmid DNA employing nuclease-poor host bacteria and

meeting the lot release specifications indicated below • DNA and markers production are performed employing high-quality restriction enzymes • All DNA fragments are de-proteinated, precipitated, desalted, adjusted in concentration with an O.D. of 1 at

260 nm equalling 50μg DNA, aliquoted and subsequently lyophilised for high storage stability. • The markers with regular increments are Eco RI-digested. This feature allows the double labelling in two

positions. The products are protected by a patent. • All markers contain 1mL gel loading buffer (TAE, glycerol, bromophenol-xylenecyanol-blue) per 50μg marker. • All markers are qualified using rigorous quality control assays. Tests regarding DNA concentration, Purity

(general, visible, genomic DNA and RNA), Homogeneity and Identity (3 enzymes) are performed for each lot.

Marker Codice Number of fragments

Fragments Range (in bp)

Quantità Number of loads

100bp DNA ladder OZB-PF00100 11 100-1000 100bp DNA ladder PLUS OZB-PF00200 17 100-5000 1kbp DNA ladder OZB-PF00300 11 500-10000 ShortRun DNA marker OZB-PF00400 5 500-2500 pBR328 Hinf I / Bgl I OZB-PF00500 12 154-2176 pUC18 Hpa II OZB-PF00600 12 26-501 pUC19 Msp I OZB-PF00700 12 26-501 pBR322 Hae III OZB-PF00800 22 8-587 λ Hind III / phi-X 174 Hae III OZB-PF00900 19 72-23130

50 μg 100

Per scegliere il marker corretto:


Catalogo 2010-11


Related products: DNA Markers Specifiche tecniche:

100bp DNA ladder (100 - 1000 bp)

Fragments: 1000 – 900 – 800 – 700 – 600 – 500 (2x) – 400 – 300 – 200 – 150 – 100 bp

Recommended for: Analysis of mid-sized DNA fragments from restriction analysis and plasmid vectors in the average size range

Features: 10 fragments with increments of 100bp with an additional 150bp fragment for the analysis of 100-200bp PCR-products. Two times concentrated 500bp-band for easy detection. Others bands are equimolar concentration (1:1 ratio). Needs 70-80mm distance in 1.2% - 1.5% agarose gels for complete separation of bands

Code: OZB-PF00100

100bp DNA ladder PLUS (100 - 5000 bp)

Fragments: 5000 – 4000 – 3000 – 2500 – 2000 – 1500 – 1000 – 900 – 800 – 700 – 600 – 500 (2x) – 400 – 300 – 200 – 150 – 100 bp

Recommended for: Analysis of mid- and large-sized DNA fragments from restriction analysis and plasmid vectors in the average size range

Features: In addition to the above mentioned 100bp-DNA ladder (60%), 40% of the total DNA-mass is an equimolar mixture of 6 high-molecular DNA fragments ranging from 1500 to 5000bp. On 1.0% - 1.5% agarose gels, the extended ladder looks like two separated, set-on-top DNA ladders

Code: OZB-PF00200

1kbp DNA ladder (500 - 10000 bp)

Fragments: 10000 – 8000 – 6000 – 5000 – 4000 – 3000 – 2500 – 2000 – 1500 – 1000 – 500 bp

Recommended for: Analysis of larger DNA fragments and PCR-products

Features: 11 fragments with increments of 2000, 1000 or 500bp. All bands are in equimolar concentration. Needs 70-800mm distance in 1.0% - 1.2% agarose gels for complete separation of bands

Code: OZB-PF00300

ShortRun DNA Marker (500 - 2500 bp)

Fragments: 2500 – 2000 – 1500 (2x) – 1000 – 500 bp

Recommended for: Quick gel separation in high throughput applications

Features: 5 fragments with 500bp increments. Needs 1.5-2.5mm distance in 1.2% - 1.5% agarose gels for complete separation of bands

Code: OZB-PF00400

pBR328 Hinf / Bgl I (154 - 2176 bp)

Fragments: 2176 – 1766 – 1230 – 1033 – 653 – 517 – 453 – 394 – 298 – 234 – 220 – 154 bp

Recommended for: Quick gel separation in high throughput applications.Analysis of mid- and small-sized DNA fragments and PCR products

Features: Easily detectable double bands of 2176 / 1766bp and 1230 / 1033bp. Used on 1.2% - 1.5% agarose gels with fast spread-up of the fragments

Code: OZB-PF00500

pUC18 Hpa II (26 - 501 bp)

Fragments: 501 – 489 – 404 – 353 – 242 – 190 – 147 – 110 – 89 – 67 – 34 – 26 bp

Recommended for: Analysis smaller PCR products

Features: Used on 1.2% - 1.5% agarose gels with fast spread-up of the fragments

Code: OZB-PF00600

pUC19 Msp I (26 - 501 bp)

Fragments: 501 – 489 – 404 – 353 – 242 – 190 – 147 – 110 – 89 – 67 – 34 – 26 bp

Recommended for: Analysis smaller PCR products

Features: Contain a series of fragments in the low molecular range. Allows quick concentration by the easily detectable 501 / 489 and 111 / 110 double fragments. Needs only short separation distance on 1.5% - 2.2% agarose gels for the 501 to 110bp fragments

Code: OZB- PF00700

pBR322 Hae III (8 - 587 bp)

Fragments: 587 – 540 – 502 – 458 – 434 – 267 – 234 – 213 – 192 – 184 – 124 – 123 – 104 – 89 – 80 – 64 – 57 – 51 – 21 – 18 – 11 – 8 bp

Recommended for: Gel analysis on 1.8% - 2.2% agarose gels

Features: Large number of fragments in the low molecular range; mainly clustered in three areas of 3-4 fragments. Contains also very small fragments, which take longer for complete separation

Code: OZB- PF00800

Lambda Hind III / phi-X 174 Hae III

Fragments: 23130 – 9416 – 6557 – 4361 – 2322 – 2027 – 1353 – 1078 – 872 – 603 – 564 – 310 – 281 – 271 – 234 – 194 – 125 – 118 – 72 bp

Recommended for: Gel analysis on 1.8% - 2.2% agarose gels.Wide range of fragment sizes

Features: Fast spread-up of the fragments on 1.2% - 1.5% agarose gels

Code: OZB- PF00900


Catalogo 2010-11


Assay Kits: GeneBlaster

Caratteristiche: Per ottimizzare gli esperimenti di trasfezione, c’è una soluzione innovativa ed efficiente che migliora i livelli di espressione genica. I Kit GeneBlasterTM sono un set di prodotti chimici destinati ad ottenere livelli di espressione del transgene elevati. Dato che l’applicazione del GeneBlasterTM kit è dipendente dal tipo di cellula e dal promotore, sono state studiate 3 diverse formulazioni:

- GeneBlasterTM Ruby: sviluppato per cellule aderenti - GeneBlasterTM Sapphire: sviluppato per le cellule aderenti, a complemento della Ruby - GeneBlasterTM Topaz: sviluppato per cellule in sospensione, soprattutto ematopoietiche.

Risultati:

Vantaggi:

• La più alta espressione genica in molte cellule • Conveniente per un ampio pannello di cellule aderenti o in sospensione • Prolunga l’espressione genica in vitro • Adatto a tutti i tipi di vettore • Protocollo Semplice e pronto all’uso • Economico


OZB-GB20011 GeneBlaster Ruby 3 x 1,5 ml

OZB-GB20012 GeneBlaster Sapphire 3 x 1,5 ml

OZB-GB20013 GeneBlaster Topaz 3 x 1,5 ml

OZB-GB20010 GeneBlaster Selection Kit (OZB-GB20011, OZB-GB20012, OZB-GB20013) 3 x 1,5 ml


Catalogo 2010-11


Assay Kits: Beta-Galactosidase Caratteristiche: Lac Z è uno dei più frequenti geni reporter usati negli esperimenti di trasfezione grazie alle sue proprietà specifiche. Infatti la proteina codificata da Lac Z, la Beta-galactosidase, idrolizza lo zucchero galattosidasi a lattosio, ed è molto stabile, resistente alle degradazioni proteolitiche e facilmente testata. Tutti i reagenti necessari sono compresi nei Kit e permettono un metodo rapido, semplice e sensibile per quantificare spettrofotometricamente il livello di espressione nelle cellule trasfettate. Due sono i Kit sviluppati: ONPG e CPRG OPNG β-Galattosidase Assay Kit Ideale per misurare quantitativamente alti livelli di espresione della β-Gal. I livelli di espressione della β-Gal attiva possono essere rapidamente misurati attraverso l’idrolisi catalitica dell’ONPG (o-nitrophenyl-β-D-galactopyranoside), substrato che dà una colorazione giallo brillante (abs 405-420nm). CPRG β-Galattosidase Assay Kit Ideale per misurare quantitativamente bassi livelli di espresione della β-Gal. I livelli di espressione della β-Gal attiva possono essere rapidamente misurati attraverso l’idrolisi catalitica del CPRG (Chlorophenol red-β-D-galactopyranoside), substrato che dà un colore rosso scuro. L’elevata sensibilità di questo substrato migliora la misurazione dell’attività della β-Gal quando l’espressione del gene reporte è bassa (abs 570-595nm). Risultati:

Vantaggi:

• Conveniente per tutti i test di trasfezione • Disponibile per colture cellulari e tessuti • Protocollo Semplice e pronto all’uso • Economico • Ottimizzato con buffer stabili

Codice Prodotto

OZB-GO10001 ONPG BGal Assay Kit

OZB-GC10002 CPRG BGal Assay Kit


Catalogo 2010-11


Assay Kits: X-Gal Staining Kit

Caratteristiche: Lac Z è uno dei più frequenti geni reporter usati negli esperimenti di trasfezione grazie alle sue proprietà specifiche. Infatti la proteina codificata da Lac Z, la Beta-galactosidase, idrolizza lo zucchero galattosidasi a lattosio, ed è molto stabile, resistente alle degradazioni proteolitiche e facilmente testata. Lo Staining X-Gal Kit consente di visualizzare in istochimica l’espressione della β-Gal, in quanto l'idrolisi di X-Gal (5-bromo-4-cloro-3-indoyl-ß-D-galattopiranoside) produce un precipitato blu. Tutti i reagenti necessari sono contenuti nel Kit il quale offre un metodo rapido, semplice e sensibile per determinare la percentuale di cellule Lac Z trasfettate.

Linee Cellulari Trasfettate Lac Z

Vantaggi:

• Conveniente per tutti i test di trasfezione • Disponibile per colture cellulari e tessuti • Protocollo Semplice e pronto all’uso • Economico • Ottimizzato con buffer stabili

Codice Prodotto

OZB-GX10003 X Gal Staining Kit


Catalogo 2010-11


Assay Kits: short hairpin RNA (shRNA) Caratteristiche: Originale short hairpin RNA (shRNA) per l’induzione del silenziamento genico. Questi shRNA sono biologicamente processati per fermentazione, frazionamento, estrazione e purificazione di cellule di lievito geneticamente modificate. Gli shRNA forniti sono puri acidi ribonucleici e non DNA plasmidico o vettori virali codificanti per shRNA. Sono ideali per gli esperimenti di silenziamento genico. Grazie alle strutture ad hairpin, questi shRNA sono davvero stabili in confronto ai siRNA. Proponiamo 2 diversi tipi di shRNA:

- shRNA GFP - shRNA Luciferase shRNA GFP activity in HeLa cells

Vantaggi:

• Sequenze validate (specifiche ed efficienti) • Elevata stabilità • Elevata purezza (>98%) • Omogeneità unica • Pronta all’uso

Codice Prodotto

OZB-SH10001 shRNA GFP

OZB-SH10002 shRNA Luciferase

OZB-SH10012 shRNA GFP + shRNA Luciferase


Catalogo 2010-11


PCR, RT-PCR, Western Blot: iNtRON Biotechnology Master Mix per PCR, RT-PCR 108 PCR Starter Kit 109 e-Myco™ 110 e-Myco Plus™ 111 WEST-one® 112


Catalogo 2010-11


Master Mix per PCR, RT-PCR Caratteristiche: Nuova gamma di prodotti "ready-to-use" per PCR, RT e RT-PCR Veloci ed efficienti, riducono il rischio di contaminazioni diminuendo il numero di manipolazioni da parte dell’operatore. Efficienza, specificità e accuratezza vengono garantite dall’elevato grado di purezza degli enzimi. Qualità dei prodotti e riproducibilità sono garantite dalla certificazione ISO 9001/14001.

2x Master Mix Solution 2x Master Mix Solution i-Taq 2x Master Mix Solution i-StarTaq 2x Master Mix Solution i-pfu

Codice: INT-25027, INT-25028 INT-25166 INT-25186

Vantaggi: • dNTPs, Taq DNA polimerasi e buffer sono contenuti in un’unica mix di reazione utilizzabile per reazioni PCR

• E’ sufficiente aliquotare la mix, aggiungere templato e primer, quindi aggiustare il volume finale con dH2O

• Efficienza, specificità o accuratezza sono privilegiate a seconda della Taq polimerasi utilizzata

Maxime PCR PreMix Maxime PCR PreMix i-Taq Maxime PCR PreMix i-StarTaq Maxime PCR PreMix i-pfu

Codice: INT-25025, INT-25026 INT-25165 INT-25185

Vantaggi: • Tutti i componenti della miscela di PCR sono aliquotati in singole provette che vengono direttamente utilizzate nelle reazioni: è sufficiente aggiungere templato e primer

• Il colorante presente nelle provette consente di caricare direttamente il campione su gel alla fine della reazione

MaximeRT/RT-PCR PreMix Maxime RT PreMix (Oligo(dT)15 Primer) Maxime RT PreMix (Random Primer)

Codice: INT-26081 INT-25082

Company A Maxime RT PreMix

Comparison of Maxime PCR PreMix (Oligo dT Primer) and Company A's RT PreMix system by amplifying 570bp DNA fragment (GAPDH).

Vantaggi: • Il kit, disponibile nelle versioni Oligo(dT) e Random Primer, consente un’efficientesintesi del primo strand di cDNA utilizzabile come substrato per PCR

• Tutti i componenti della miscela di reazione sono aliquotati in singole provette: èsufficiente aggiungere RNA e dH2O

Maxime RT-PCR PreMix

Codice: INT-25131 Company A Maxime RT PreMix

Comparison of Company A's RT-PCR PreMix system and Maxime RT-PCR PreMix kit by diagnosis of Newcastle disease (common).

Vantaggi: • Il kit consente con una sola manipolazione la sintesi del primo strand di cDNA e l’amplificazione per PCR

• La miscela di reazione è aliquotata in singole provette: è sufficiente aggiungere RNA, primer e dH2O

• Il colorante presente nelle provette consente di caricare direttamente il campione su gel alla fine della reazione

• L’utilizzo dei prodotti OptiScript RT System per la retrotrascrizione e i-StarTaq per l’amplificazione garantisce elevata efficienza


Catalogo 2010-11


PCR Starter Kit

Caratteristiche:

MS-1 e MS-2 PCR Starter Kit sono stati studiati per aiutare l’utilizzatore nella scelta delle migliori condizioni di PCR.

Quando si esegue una PCR, i risultati possono variare in funzione delle diverse condizioni PCR e dei fattori utilizzati,

come la temperatura di annealing, il tempo di estensione, ecc.., i quali possono influenzare l’amplificazione del gene

target. Questo Kit consente di ottenere delle condizioni di PCR ottimali utilizzando diversi tipi di Taq DNA Polymerase.

PCR Starter Kit (MS-1 Type) è studiato per risolvere le difficoltà che si incontrano nella selezione della Taq DNA

Polymerase e il settaggio delle perfette condizioni di PCR.

Vantaggi: - Screening delle migliori condizioni di PCR in un solo test

- Consente di coordinare le varie condizioni PCR in quanto consiste di 4 diverse Taq DNA Polymerase


INT-25141 PCR Starter Kit (MS-1 Type) 96 tubi

MS-2 Type Starter Kit consiste in 32 differenti componenti PCR e fornirà 32 differenti risultati PCR in un solo

esperimento in modo da poter controllare ed eventualmente cambiare le condizioni sperimentali; il Kit consente quindi

di ottenere i risultati desiderati in un tempo molto breve


INT-25142 PCR Starter Kit (MS-2 Type) 96 tubi

MS-1 Type Starter Kit consiste in differenti tipi di Taq DNA

Polymerase per tubo, come “premium standard i-Taq™ DNA

Polymerase”, “i-StarTaq™ DNA Polymerase (hot-start)”, “i-MAX™

II DNA Polymerase (long and high-fidelity)” e “i-StarMAX™ II

DNA Polymerase (hot start, long and high-fidelity)”.

MS-2 Type Starter Kit è stato studiato per ottenere le migliori condizioni di PCR velocemente ed efficacemente. Il tipo e la concentrazione dei primers e il tempo di reazione della PCR possono essere decisi dall’utilizzatore, mentre le altre condizioni PCR come la composizione del buffer, la concentrazione di MgCl2 , il tipo e la concentrazione della DNA polymerase vengono forniti nel Kit come premix. Una volta individuato il numero della composizione premix ideale, è possibile, su richiesta, creare un kit con la composizione prescelta.


Catalogo 2010-11


e-Myco Caratteristiche: e-Myco™ Mycoplasma PCR Detection Kit: per rilevare le contaminazioni da Mycoplasma Il Mycoplasma è un contaminante delle colture cellulari che, pur non danneggiando in maniera visibile la cellula, ne altera alcuni parametri fondamentali, quali ad esempio metabolismo, crescita, sintesi di DNA, RNA e proteine. Procedura:

Risultati:

Mycoplasma detection performed with e-Myco MycoplasmaDetection kit using isolated genomic DNA of infected K562 cells. Template range: from 200 ng (lane1) to 6.3 pg (lane 16)

Mycoplasma detection performed with e-MycoMycoplasma Detection kit using K562-infected celllysates. Cell number range: 1x106 to 1x103

Vantaggi:

• Efficiente: Basato sull’amplificazione per PCR delle sequenze conservate rRNA 16S. Lo stato di contaminazione viene verificato con elettroforesi dei frammenti amplificati (270bp)

• Ampio spettro: rileva la contaminazione di molte specie di Mycoplasma, tra cui le cinque più comuni: Il sequenziamento dei prodotti di PCR consente di determinare la specie di appartenenza.

• Semplice e veloce: tutti i componenti della miscela di reazione sono aliquotati in singole provette. E’ sufficiente aggiungere il campione sotto forma di lisato cellulare (bollitura 5 min) o di DNA genomico. Dopo la reazione di PCR il campione è pronto per essere caricato su gel. Disponibile per 96 reazioni.

• Sensibile: la contaminazione viene rilevata in campioni contenenti 5x104 cellule oppure 50-100ng di DNA genomico.


INT-25233 e-Myco™ PCR Mycoplasma Detection Kit 96 Tubes

Bollitura (95°, 5 min)

Estrazione DNA genomico

Sequenziamento dei Prodotti PCR

Identifica la specie di Mycoplasma

contaminante Rileva la contaminazione

da Mycoplasma

e-Myco™ Mycoplasma Detection Kit

5x104 cells


Catalogo 2010-11


e-Myco Plus

Caratteristiche:

NUOVO e-Myco™ Plus Mycoplasma PCR Detection Kit • Ampio range di specie di Mycoplasma detectabili; non solo le 5 più comuni specie di Mycoplasma infettanti le

colture, ma anche altre numerose specie: oltre 8 generi e 209 specie. • Riconosce anche M. pneumoniae e M. genitalium • Possibilità di verificare facilmente l’efficacia della reazione PCR controllando l’amplificazione dal campione controllo. Procedura:

Risultati:

Vantaggi: • Detection di 8 generi e 209 specie di contaminanti di colture cellulari • Triplo controllo:

CAMPIONE DI CONTROLLO 570 bp: indica la corretta preparazione del campione BANDA TARGET 260 bp: parametro di infezione Mycoplasmatica CONTROLLO INTERNO 160 bp: parametro di controllo di ogni eventuale problema che potrebbe sorgere durante l’amplificazione PCR


INT-25234 e-Myco™ Plus PCR Mycoplasma Detection Kit 96 Tubes


Catalogo 2010-11


WEST-one Caratteristiche/vantaggi: WEST-one® Western Blot Detection System: il nuovo sistema di rivelazione ECL pronto all’uso

• Rapido: il prodotto consiste in un’unica soluzione premiscelata; non è necessario miscelare il substrato e l’enhancer

• Comoda confezione spray: è sufficiente spruzzare WEST-one® sulla membrana per eseguire la reazione di ECL. Ogni spruzzo corrisponde a 500 µl

• Economico: due spruzzi (1 ml) sono sufficienti per lo sviluppo di un filtro. La confezione da 100 ml è utilizzabile per circa 100 filtri

• Elevata sensibilità: WEST-one® è in grado di rilevare quantità di antigene inferiori a 1-2 pg • Alta risoluzione: WEST-one® è in grado di generare segnali ad alto contrasto • Veloce: il tempo di incubazione è compreso tra 1 e 10 minuti • Stabile: garantito per mantenere inalterata la sua efficienza per almeno un anno

Procedura/risultati:

HRP

substrato

enhancer

stabilizzante

+

+

HRP

substrato

enhancer

stabilizzante

+

+


INT-16031 WEST-one Western Blot Detection (Spray Type) 100 ml INT-16032 WEST-one Western Blot Detection (Spray Type) 200 ml INT-16033 WEST-one Western Blot Detection (Nozzle Type) 200 ml


Catalogo 2010-11


Analisi delle interazioni proteiche: Hypromatrix AntibodyArrays 114

Signal Transduction "AntibodyArray™" Apoptosis "AntibodyArray™" Cell Cycle "AntibodyArray™" Custom "AntibodyArray™" Anticorpi di rivelazione

Staining AntibodyArrays 122


Catalogo 2010-11


AntibodyArrays Caratteristiche: AntibodyArray™, lo strumento perfetto per:

• Lo screening delle interazioni tra proteine • Studiare le modifiche traslazionali, specialmente la fosforilazione • Esaminare i patterns di espressione

"AntibodyArray™", prodotti unici e innovativi: membrane sulle quali sono stati posti sino a 400 specifici anticorpi monoclonali. Il principio del metodo è veramente semplice: dopo aver provocato la modifica conformazionale della proteina d’interesse con stimoli vari od ottenuta una nuova proteina, si blocca chimicamente il lisato cellulare e lo s'ibridizza sulla speciale membrana dove sono depositati gli anticorpi monoclonali. La proteina sarà quindi catturata da differenti anticorpi. Valutando le posizioni di cattura, si risale agli anticorpi, determinando così le modificazioni avvenute a seguito dello stimolo o le varie interazioni di una nuova proteina. Procedura:

Risultati:

Analisi di STAT1 in cellule Hela

Determinazione delle interazioni

di Stat1 in condizioni di controllo.

Determinazione delle interazioni

di Stat1 dopo un’ora di trattamento delle cellule con

TNF-alpha.

Vantaggi:

Questo semplice sistema sostituisce il più tradizionale yeast two-hybrid system, apportando numerosi vantaggi: • Tempo d’esecuzione: 5-6 ore anziché 2 o 3 mesi • Costi sicuramente inferiori: 500-2000 euro rispetto ai 20.000 del two-hybrid system • Facile esecuzione (il two-hybrid necessita della conoscenza della genetica dei lieviti) • Non sono necessarie strumentazioni particolari • Le interazioni rilevate sono fisiologicamente importanti • Falsi positivi minimizzati, notevolmente inferiori rispetto al two-hybrid


HYP-HM3000 Signal Transduction "AntibodyArray™" (membrana con 400 anticorpi per il signal transduction) 1 Array HYP-HM3000T Trial "AntibodyArray™" 1 Array HYP-HM4000 Apoptosis "AntibodyArray™" (membrana con 150 anticorpi contro le proteine dell'apoptosi) 1 Array HYP-HM5000 Cell Cycle "AntibodyArray™" (membrana con 60 anticorpi contro le proteine del ciclo cellulare) 1 Array HYP-HM6000 Custom AntibodyArray™ (setup fee) 1 Array


Catalogo 2010-11


AntibodyArrays: Signal Transduction Each Signal Transduction AntibodyArray™ contains 400 high quality antibodies against well-studied signaling proteins. Signal Transduction AntibodyArray™systems are generally used in the following applications:

• To screen novel protein-protein interactions. For example, when a scientist clones a new gene and he/she wishes to find out its interacting partners, an AntibodyArray™is the tool of choice

• To find out changes in protein-protein interactions under different conditions, such as in different cell types, different developmental stages and different diseases

• To examine changes in protein tyrosine phosphorylation under different conditions


HYP-HM3000 Signal Transduction "AntibodyArray™" (membrana con 400 anticorpi per il signal transduction) 1 Array HYP-HM3000T Trial "AntibodyArray™" 1 Array

Anticorpi presenti sulla membrana Signal Transduction "AntibodyArray™" (001-200)

001 14-3-3 041 BRCA2 081 Cdc34 121 Dynamin II 161 GATA-1

002 c-Abl 042 Brk 082 CDC42GAP 122 E2F1 162 GATA-2

003 ACINUS 043 Brm 083 Cdk1/Cdc2 123 EGFR 163 GATA-3

004 ACINUS-p23 044 BRUCE 084 Cdk2 124 Egr-1 164 G-CSF R

005 AFAP 045 Btk 085 Cdk4 125 Egr-2 165 gp130

006 AIF 046 C/EBP beta 086 Chk 126 Egr-3 166 Granzyme B

007 Akt 1/2 047 Cadherin-5 087 C-IAP1 127 Elongin A 167 GRB14

008 ALK 048 E-Cadherin 088 C-IAP2 128 EphA1 168 GRB2

009 Amphiphysin 049 N-Cadherin 089 CIDE-A 129 EphA4 169 GRB7

010 Ankyrin 050 P-Cadherin 090 CIDE-B 130 EphB1 170 GRK 2

011 Bin 1 051 BLCAM 091 Clathrin 131 eps8 171 GSK-3 alpha

012 Annexin VI 052 HCAM 092 Clusterin 132 erbB2 172 HDAC1

013 ANT 053 ICAM-1 093 Connexin26 133 erbB3 173 hdlg

014 Apaf1 054 PECAM-1 094 Connexin43 134 erbB4 174 Ne-dlg

015 APC 055 VCAM-1 095 Cortactin 135 ERK1 175 hIL-4 sR

016 ARC 056 CAS 096 CPAN 136 ERK2 176 hILP

017 Axl 057 Caspase1 097 CREB 137 Estrogen R a 177 Hrk

018 B7-1 058 Caspase2 098 CREM-1 138 Ets-1/2 178 HS1

019 B7-2 059 Caspase3 099 Crk 139 Ezrin 179 HSP-70

020 Bad 060 Caspase4 100 Csk 140 FADD 180 ICSBP

021 Bak 061 Caspase5 101 CUL-1 141 FAF-1 181 Id1

022 Bax 062 Caspase6 102 Cyclin A 142 FAK 182 IEXs/l

023 BOK 063 Caspase7 103 Cyclin B 143 Fas 183 IFN-a R a

024 NBK 064 Caspase8 104 Cyclin D3 144 FasL 184 IFN-g R a

025 Bag-1 065 Caspase9 105 Cyclin E 145 FAST 185 IL 1 R1

026 Bcl-2 066 Caspase10 106 Cyclin H 146 FGFR1 186 IL 2 R alpha

027 Bcl-w 067 a-Catenin 107 cytochrome C 147 FGFR2 187 IL 2 R, beta

028 Bcl-xS/L 068 b-Catenin 108 DAXX 148 FGFR3 188 IL 2 gamma

029 A1 069 r-catenin 109 DCC 149 FGFR4 189 IL 3

030 Mcl-1 070 c-Cbl 110 Desmoglein 150 FHIT 190 IL4R a

031 Bcl-3 071 CBP 111 DFF45/ICAD 151 FLASH 191 ING1-p33

032 Bcl-6 072 CD 3 epsilon 112 DIVA 152 FLIPs/l 192 Insulin R b

033 BID 073 CD27 113 DMBT1 153 Flt-3/2 193 Integrin-alphal

034 Bim 074 CD28 114 DMC1 154 Flt-4 194 Integrin a 5

035 BM28 075 CD29 115 DcR1 155 c-Fos 195 Integrin a V

036 BMX 076 CD30 116 DR2 156 Frizzled 196 Integrin b 1

037 BRAMP2 077 CD40 117 DR3 157 GADD34 197 Integrin b 3

038 BAP1 078 CD45 118 DR4 158 GADD45 198 IRAK

039 BARD1 079 Cdc6 119 DR5 159 GADD153 199 IRF1

040 BRCA1 080 Cdc25A 120 Dynamin 160 GAK 200 IRF2


Catalogo 2010-11


AntibodyArrays: Signal Transduction Anticorpi presenti sulla membrana Signal Transduction "AntibodyArray™" (201-400)

201 IRS-1 241 Ikappa B-a 281 PDGF Receptor a 321 Ret 361 SURVIVIN

202 ISGF3 gamma 242 p-Ikappa B-a 282 PDGF Receptor b 322 Rho A 362 Syk

203 Jak1 243 Ikappa B-b 283 Phospholipase Cr 323 RICK 363 Syntaxin6

204 Jak2 244 Ikappa B-r 284 Phospholipase D 324 RIP 364 TANK

205 Jak3 245 Ikappa B-e 285 PI3kinase P85 325 Ron alpha 365 TCR apha

206 JNK1,2,3 246 Ikappa B kinase a 286 PKC α,β,χ 326 Rsk-1 366 TCR beta

207 p-JNK1,2,3 247 Ikappa B kinase b 287 Polo-like kinase 327 Sam68 367 TDAG51

208 c-Jun 248 Nibrin 288 PP1,2A,2B,PPX 328 E-selectin 368 TGFb R1

209 KAP 249 NIK 289 pp120 329 L-Selectin 369 TGFb R2

210 c-Kit 250 nip1 290 PSD-95 330 P-Selectin 370 Thyroid R a1

211 L1 251 nip2 291 PTEN 331 SHC 371 TIA-1

212 LGI1 252 nip3 292 SH-PTP 332 SHIP 372 TIAR

213 LIFR 253 eNOS 293 PTP1B 333 Sik 373 TNFR1

214 Mad-1 254 iNOS 294 SH-PTP2 334 SIVA 374 TNFR2

215 MAD2 255 nNOS 295 PYK2 335 Smad (1/2/3) 375 TOSO

216 Maspin 256 Notch 296 Rab3 336 Smad 4 376 TRADD

217 Max 257 Ntk 297 Rab5 337 SOCS-1 377 TRAF1

218 MDA-7 258 Nurr 1 298 Rab11 338 SODD 378 TRAF2

219 MDM2 259 Ob Receptor 299 Rab27 339 Sos1/2 379 TRAF3

220 Mdr (H-241) 260 p16 300 Rac1 340 sp1 380 TRAF4

221 MEF2 261 p19Skp1 301 RACK1 341 sp2 381 TRAF5

222 MEK1 262 p21WAF1/CIP1 302 Rad51 342 Spectrin 382 TRAF6

223 MEKK1 263 p27 303 Rad52 343 Blk 383 TRAIL

224 MEKK2 264 p35 304 c-Raf-1 344 c-Fgr 384 TrkA, B, C

225 Menin 265 p36 305 RAIDD 345 fyn 385 TSG101

226 Met 266 p38 MAPK 306 RalA 346 Lck 386 TTK

227 MGMT 267 p45 skp2 307 Ran 347 Lyn 387 Tuberin

228 MMp-3 268 p53 308 Rap1 348 c-Src 388 Tyk2

229 MMp-9 269 p55 CDC 309 Rap2 349 Yes 389 VASP

230 Mos 270 p57 (Kip2) 310 RAR r 350 SRF 390 Vav

231 Myc (c-Myc) 271 P63 311 RXR a,b,r 351 STAM 391 VDAC1

232 MyoD 272 P73 312 Ras 352 Stat1 392 VDR

233 NCK 273 p130Cas 313 Ras-GAP 353 p-Stat1 393 VEGFR1

234 NF-1 274 p300 314 Rb (p107) 354 Stat2 394 VEGFR2

235 NF1GRP 275 Frizzled 315 Rb (p110) 355 Stat3 395 VHL

236 NF2 276 PAR-4 316 Rb2 (130) 356 p-Stat3 396 WT

237 NFATC 277 PARP 317 RBBP 357 Stat4 397 XRCC4

238 NF-kappa B 50 278 Pax-5 318 Rbx1 & 2 358 Stat5a 398 YY1

239 NF-kappa B 52 279 Paxillin 319 c-Rel 359 Stat5b 399 ZAP70 Kinase

240 NF-kappa B p65 280 PCNA 320 Rel B 360 Stat6 400 ZO-1


Catalogo 2010-11


AntibodyArrays: Apoptosis Each Apoptosis AntibodyArray™ contains high quality antibodies against 150 proteins regulating apoptosis. Apoptosis AntibodyArray™ systems are generally used in the following applications:

• To screen novel protein-protein interactions that regulate cell death • To find out changes in protein-protein interactions during apoptosis • To examine changes in protein tyrosine phosphorylation under apoptosis conditions


HYP-HM4000 Apoptosis "AntibodyArray™" (membrana con 150 anticorpi contro le proteine dell'apoptosi) 1 Array Anticorpi presenti sulla membrana Apoptosis "AntibodyArray™" (001-150)

001 ACINUS 031 Caspase3 061 FADD 091 MEKK2 121 RbP130 (Rb2)

002 ACINUS-p23 032 Caspase4 062 FAF-1 092 Menin 122 RBBP

003 AFAP 033 Caspase5 063 Fas 093 MGMT 123 c-Rel

004 AIF 034 Caspase6 064 FasL 094 c-Myc 124 RICK

005 Bin 1 035 Caspase7 065 FAST 095 NF1GRP 125 RIP

006 ANT 036 Caspase8 066 FHIT 096 NF2 126 SIVA

007 Apaf1 037 Caspase9 067 FLASH 097 NF-kappa B 50 127 SODD

008 APC 038 Caspase10 068 FLIPs/l 098 NF-kappa B 52 128 Stat1

009 ARC 039 CD27 069 GADD34 099 NF-kappa B p65 129 SURVIVIN

010 Bad 040 CD30 070 GADD45 100 Ikappa B-a 130 TANK

011 Bak 041 CD40 071 GADD153 101 Ikappa B-b 131 TDAG51

012 Bax 042 C-IAP1 072 Granzyme B 102 Ikappa B kinase a 132 TIA-1

013 BOK 043 C-IAP2 073 hdlg 103 Ikappa B kinase b 133 TIAR

014 NBK 044 CIDE-A 074 Ne-dlg 104 Nibrin 134 TNFR1

015 Bag-1 045 CIDE-B 075 hILP 105 NIK 135 TNFR2

016 Bcl-2 046 Clusterin 076 Hrk 106 nip1 136 TOSO

017 Bcl-w 047 CPAN 077 IEXs/l 107 nip2 137 TRADD

018 Bcl-xS/L 048 cytochrome C 078 ING1-p33 108 nip3 138 TRAF1

019 A1 049 DAXX 079 Jak1 109 p53 139 TRAF2

020 Mcl-1 050 DCC 080 Jak2 110 P63 140 TRAF3

021 BID 051 DFF45/ICAD 081 Jak3 111 P73 141 TRAF4

022 Bim 052 DIVA 082 JNK1,2,3 112 PAR-4 142 TRAF5

023 BAP1 053 DMBT1 083 p-JNK1,2,3 113 PARP 143 TRAF6

024 BARD1 054 DMC1 084 LGI1 114 PSD-95 144 TRAIL

025 BRCA1 055 DcR1 085 Maspin 115 PTEN 145 TSG101

026 BRCA2 056 DR2 086 MDA-7 116 Rad51 146 Tuberin

027 BRUCE 057 DR3 087 MDM2 117 Rad52 147 Tyk2

028 CAS 058 DR4 088 MEF2 118 RAIDD 148 VDAC1

029 Caspase1 059 DR5 089 MEK1 119 Rb(p107) 149 VHL

030 Caspase2 060 Elongin A 090 MEKK1 120 Rb (p110) 150 XRCC4


Catalogo 2010-11


AntibodyArrays: Cell Cycle Each Cell Cycle AntibodyArray™ contains high quality antibodies against 60 proteins regulating cell cycle. Cell Cycle AntibodyArray™ systems are generally used in the following applications:

• To screen novel protein-protein interactions that regulate cell cycle • To find out changes in protein-protein interactions during cell cycle • To examine changes in protein tyrosine phosphorylation during cell cycle phases


HYP-HM5000 Cell Cycle "AntibodyArray™" (membrana con 60 anticorpi contro le proteine del ciclo cellulare) 1 Array Anticorpi presenti sulla membrana Cell Cycle "AntibodyArray™" (001-060)

001 BM28 013 Cyclin A 025 Ne-dlg 037 p21WAF1/CIP1 049 Rad52

002 BRCA1 014 Cyclin B 026 ING1-p33 038 p27 050 Rb (p107)

003 BRCA2 015 Cyclin D3 027 KAP 039 p35 051 Rb (p110)

004 Brm 016 Cyclin E 028 MAD2 040 p36 052 RbP130 (Rb2)

005 Cdc6 017 Cyclin H 029 Mos 041 p53 053 RBBP

006 Cdc25A 018 DMC1 030 NF-1 042 p55 CDC 054 Rbx1 & 2

007 Cdc34 019 E2F1 031 NF-kappa B, p65 043 p57 055 Sam68

008 Cdk1/Cdc2 020 GADD34 032 Ikappa b, a 044 P63 056 Stat1

009 Cdk2 021 GADD45 033 Nibrin 045 P73 057 p-Stat1

010 Cdk4 022 GADD153 034 p16 046 PCNA 058 Stat3

011 Chk 023 GAK 035 p19Skp1 047 Polo-like kinase 059 TTK

012 CUL-1 024 hdlg 036 p45 skp2 048 Rad51 060 XRCC4


Catalogo 2010-11


AntibodyArrays: Custom


HYP-HM6000 Custom AntibodyArray™ (setup fee) 1 Array HYP-HM6AAA Anticorpi per Custom AntibodyArray™ 1 anticorpo HYP-HM1AAA Anticorpi primari (disponibili separatamente) 1 x 20 µg

Anticorpi per Custom AntibodyArray/Anticorpi primari (001-200)

001 14-3-3 041 BRCA2 081 Cdc34 121 Dynamin II 161 GATA-1

002 c-Abl 042 Brk 082 CDC42GAP 122 E2F1 162 GATA-2

003 ACINUS 043 Brm 083 Cdk1/Cdc2 123 EGFR 163 GATA-3

004 ACINUS-p23 044 BRUCE 084 Cdk2 124 Egr-1 164 G-CSF R

005 AFAP 045 Btk 085 Cdk4 125 Egr-2 165 gp130

006 AIF 046 C/EBP beta 086 Chk 126 Egr-3 166 Granzyme B

007 Akt 1/2 047 Cadherin-5 087 C-IAP1 127 Elongin A 167 GRB14

008 ALK 048 E-Cadherin 088 C-IAP2 128 EphA1 168 GRB2

009 Amphiphysin 049 N-Cadherin 089 CIDE-A 129 EphA4 169 GRB7

010 Ankyrin 050 P-Cadherin 090 CIDE-B 130 EphB1 170 GRK 2

011 Bin 1 051 BLCAM 091 Clathrin 131 eps8 171 GSK-3 alpha

012 Annexin VI 052 HCAM 092 Clusterin 132 erbB2 172 HDAC1

013 ANT 053 ICAM-1 093 Connexin26 133 erbB3 173 hdlg

014 Apaf1 054 PECAM-1 094 Connexin43 134 erbB4 174 Ne-dlg

015 APC 055 VCAM-1 095 Cortactin 135 ERK1 175 hIL-4 sR

016 ARC 056 CAS 096 CPAN 136 ERK2 176 hILP

017 Axl 057 Caspase1 097 CREB 137 Estrogen R a 177 Hrk

018 B7-1 058 Caspase2 098 CREM-1 138 Ets-1/2 178 HS1

019 B7-2 059 Caspase3 099 Crk 139 Ezrin 179 HSP-70

020 Bad 060 Caspase4 100 Csk 140 FADD 180 ICSBP

021 Bak 061 Caspase5 101 CUL-1 141 FAF-1 181 Id1

022 Bax 062 Caspase6 102 Cyclin A 142 FAK 182 IEXs/l

023 BOK 063 Caspase7 103 Cyclin B 143 Fas 183 IFN-a R a

024 NBK 064 Caspase8 104 Cyclin D3 144 FasL 184 IFN-g R a

025 Bag-1 065 Caspase9 105 Cyclin E 145 FAST 185 IL 1 R1

026 Bcl-2 066 Caspase10 106 Cyclin H 146 FGFR1 186 IL 2 R alpha

027 Bcl-w 067 a-Catenin 107 cytochrome C 147 FGFR2 187 IL 2 R, beta

028 Bcl-xS/L 068 b-Catenin 108 DAXX 148 FGFR3 188 IL 2 gamma

029 A1 069 r-catenin 109 DCC 149 FGFR4 189 IL 3

030 Mcl-1 070 c-Cbl 110 Desmoglein 150 FHIT 190 IL4R a

031 Bcl-3 071 CBP 111 DFF45/ICAD 151 FLASH 191 ING1-p33

032 Bcl-6 072 CD 3 epsilon 112 DIVA 152 FLIPs/l 192 Insulin R b

033 BID 073 CD27 113 DMBT1 153 Flt-3/2 193 Integrin-alphal

034 Bim 074 CD28 114 DMC1 154 Flt-4 194 Integrin a 5

035 BM28 075 CD29 115 DcR1 155 c-Fos 195 Integrin a V

036 BMX 076 CD30 116 DR2 156 Frizzled 196 Integrin b 1

037 BRAMP2 077 CD40 117 DR3 157 GADD34 197 Integrin b 3

038 BAP1 078 CD45 118 DR4 158 GADD45 198 IRAK

039 BARD1 079 Cdc6 119 DR5 159 GADD153 199 IRF1

040 BRCA1 080 Cdc25A 120 Dynamin 160 GAK 200 IRF2


Catalogo 2010-11


AntibodyArrays: Custom Anticorpi per Custom AntibodyArray/Anticorpi primari (201-400)

201 IRS-1 241 Ikappa B-a 281 PDGF Receptor a 321 Ret 361 SURVIVIN

202 ISGF3 gamma 242 p-Ikappa B-a 282 PDGF Receptor b 322 Rho A 362 Syk

203 Jak1 243 Ikappa B-b 283 Phospholipase Cr 323 RICK 363 Syntaxin6

204 Jak2 244 Ikappa B-r 284 Phospholipase D 324 RIP 364 TANK

205 Jak3 245 Ikappa B-e 285 PI3kinase P85 325 Ron alpha 365 TCR apha

206 JNK1,2,3 246 Ikappa B kinase a 286 PKC α,β,χ 326 Rsk-1 366 TCR beta

207 p-JNK1,2,3 247 Ikappa B kinase b 287 Polo-like kinase 327 Sam68 367 TDAG51

208 c-Jun 248 Nibrin 288 PP1,2A,2B,PPX 328 E-selectin 368 TGFb R1

209 KAP 249 NIK 289 pp120 329 L-Selectin 369 TGFb R2

210 c-Kit 250 nip1 290 PSD-95 330 P-Selectin 370 Thyroid R a1

211 L1 251 nip2 291 PTEN 331 SHC 371 TIA-1

212 LGI1 252 nip3 292 SH-PTP 332 SHIP 372 TIAR

213 LIFR 253 eNOS 293 PTP1B 333 Sik 373 TNFR1

214 Mad-1 254 iNOS 294 SH-PTP2 334 SIVA 374 TNFR2

215 MAD2 255 nNOS 295 PYK2 335 Smad (1/2/3) 375 TOSO

216 Maspin 256 Notch 296 Rab3 336 Smad 4 376 TRADD

217 Max 257 Ntk 297 Rab5 337 SOCS-1 377 TRAF1

218 MDA-7 258 Nurr 1 298 Rab11 338 SODD 378 TRAF2

219 MDM2 259 Ob Receptor 299 Rab27 339 Sos1/2 379 TRAF3

220 Mdr (H-241) 260 p16 300 Rac1 340 sp1 380 TRAF4

221 MEF2 261 p19Skp1 301 RACK1 341 sp2 381 TRAF5

222 MEK1 262 p21WAF1/CIP1 302 Rad51 342 Spectrin 382 TRAF6

223 MEKK1 263 p27 303 Rad52 343 Blk 383 TRAIL

224 MEKK2 264 p35 304 c-Raf-1 344 c-Fgr 384 TrkA, B, C

225 Menin 265 p36 305 RAIDD 345 fyn 385 TSG101

226 Met 266 p38 MAPK 306 RalA 346 Lck 386 TTK

227 MGMT 267 p45 skp2 307 Ran 347 Lyn 387 Tuberin

228 MMp-3 268 p53 308 Rap1 348 c-Src 388 Tyk2

229 MMp-9 269 p55 CDC 309 Rap2 349 Yes 389 VASP

230 Mos 270 p57 (Kip2) 310 RAR r 350 SRF 390 Vav

231 Myc (c-Myc) 271 P63 311 RXR a,b,r 351 STAM 391 VDAC1

232 MyoD 272 P73 312 Ras 352 Stat1 392 VDR

233 NCK 273 p130Cas 313 Ras-GAP 353 p-Stat1 393 VEGFR1

234 NF-1 274 p300 314 Rb (p107) 354 Stat2 394 VEGFR2

235 NF1GRP 275 Frizzled 315 Rb (p110) 355 Stat3 395 VHL

236 NF2 276 PAR-4 316 Rb2 (130) 356 p-Stat3 396 WT

237 NFATC 277 PARP 317 RBBP 357 Stat4 397 XRCC4

238 NF-kappa B 50 278 Pax-5 318 Rbx1 & 2 358 Stat5a 398 YY1

239 NF-kappa B 52 279 Paxillin 319 c-Rel 359 Stat5b 399 ZAP70 Kinase

240 NF-kappa B p65 280 PCNA 320 Rel B 360 Stat6 400 ZO-1


Catalogo 2010-11


AntibodyArrays: HRP-Conjugated Antibodies Anticorpi di rivelazione

Codice Prodotto Applicazione Confezione

HYP-HM2010 Anti-GST HRP conjugated Interactions 20 µg HYP-HM2020 Anti-GFP HRP conjugated Interactions 20 µg HYP-HM2030 Anti-c-Myc HRP conjugated Interactions 20 µg HYP-HM2040 Anti-p-Tyrosine HRP conjugated Interactions 20 µg HYP-HM2050 Anti-HA HRP conjugated Interactions 20 µg HYP-HM2060 Anti-His HRP conjugated Phosphorylations 20 µg HYP-HM2070 Anti-p-Serine HRP conjugated Phosphorylations 20 µg HYP-HM2090 Anti-p-Threonine HRP conjugated Phosphorylations 20 µg


Catalogo 2010-11


AntibodyArrays: Staining Caratteristiche: Each Staining AntibodyArray™ contains 100 high quality antibodies against well-studied signaling proteins. The antibodies are arrayed onto a nitrocellulose membrane with a proprietary technique to completely retain the biological activities such as the binding of respective antigens. When the array comes in contact with cells, the array support can be removed with Dissociable AntibodyArray™ Technology. The staining of each antibody on the cells can be observed. Applications Staining AntibodyArray™ are generally used in the following applications:

• To measure the expression level of multiple proteins simultaneously. • To observe the sub-cellular locations of each proteins.

Product Each AntibodyArray™ contains 100 antibodies immobilized on a nitrocellulose membrane. The list of the antibodies on the array is included in the Instruction Manual. Applicable Species Human, mouse and rat. This array can be used in the study of human, mouse and rat systems. The majority of the antibodies on the array recognize human, mouse and rat proteins. Cross-reactivity to other species is not known. Procedura:

Risultati:

Codice Prodotto Utilità

HYP-HM8000 AntibodyArray™ Staining Apparatus Facilitate the contact between Staining AntibodyArray™ and cells

HYP-HM8020 Motorized Array Stage for upright microscope Enable automated data collection

HYP-HM8021 Motorized ArrayStage for inverted microscope Enable automated data collection

HYP-HM8010 ArrayStage control program Control the ArrayStage

HYP-HM8100 Staining "AntibodyArray" (100 anticorpi)

HYP-HM8900 Trial Staining "AntibodyArray"

HYP-HM2101 Alexa 488 Goat anti-rabbit Fluorescent labeled secondary antibody

HYP-HM2105 Alexa 488 Goat anti-mouse Fluorescent labeled secondary antibody


Catalogo 2010-11


Kit estrazione acidi nucleici: Omega Bio-Tek Prodotti Principali 124 Kit per Plasmidi 127 Kit per DNA Genomico 130 Kit per RNA 132 Kit per sistemi di purificazione DNA/RNA 134


Catalogo 2010-11


Prodotti Principali Omega Bio-Tek Purification of Genomic DNA form stool samples

The E.Z.N.A.® Stool DNA Kit allows rapid and reliable isolation of high-quality total DNA from fresh and frozen stool samples. STL2 Buffer presented in the kit is specially designed to remove inhibitory substances from stool samples.

• Quality: High quality DNA suitable for most application • Safe: No phenol/chloroform extraction • Reliable: Consistent yields at unsurpassed quality

Purification of Genomic DNA from Soil samples

Soil samples typically contain many compounds such as humic acid that can inhibit downstream enzymatic reactions. The E.Z.N.A.® Soil DNA Kits is comprised of a novel proprietary method for isolating genomic DNA from environmental samples containing high humic acid content. The isolated DNA has high level purity allowing for most downstream applications such as PCR.

• Speed: DNA isolation in under 1 hour • Reliability: Optimized buffers guarantee pure DNA every time • Quality: Purified DNA Suitable for any application

Purification of Genomic DNA from Small amount samples

E.Z.N.A.® Micro-Spin Tissue DNA kit provides a rapid and easy method for the isolation of genomic DNA from small amounts of samples for consistent PCR and others analytical tests. This kit uses the reversible binding properties of HiBind® matrix, a new silica-based material, combined with the speed of mini-column spin technology. This kit is suitable for isolation DNA from 1-100 µl blood, 0,1-10 mg animal tissues and other forensic samples such as sperm, dried blood, chewing gum and etc.

• Quality: High quality DNA suitable for most application • Safe: No phenol/chloroform extraction • Reliable: Consistent yields at unsurpassed quality • Lower elution volumes: DNA can be eluted with 15-100 µl sterile deionized water

2,5 mg Genomic DNA from plant with magnetic beads

E.Z.N.A.® Mag-Bind Plant Maxi Kit allow the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant species and tissues. The system combines the higher nucleic acid-binding properties of OBI's Magbead particle with the specialty of SP plant buffer system to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This system provides superior quality and purity to most current plant isolation methods. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. Up to 2,5 mg high molecular weight genomic DNA can be prepared in less than 1 hour. Purification of Genomic DNA from 10 ml blood with magnetic beads

E.Z.N.A.® Mag-Bind® Blood DNA Midi Kit allows rapid and reliable isolation of high-quality genomic DNA from 10 ml blood with magnetic Beads. This method can be used for the preparation of genomic DNA from blood, buffy coat, serum, plasma and cultured cells. The kit allows for the simultaneous processing of single or multiple samples in under 1 hr. Purification of Genomic DNA from 30 ml blood with magnetic beads

E.Z.N.A.® Mag-Bind® Blood DNA Maxi Kit allows rapid and reliable isolation of high-quality genomic DNA from 30 ml blood with magnetic Beads. This method can be used for the preparation of genomic DNA from blood, buffy coat, serum, plasma and cultured cells. The kit allows for the simultaneous processing of single or multiple samples in under 1 hr. Purification of Total RNA from Small amount samples

The E.Z.N.A.® Micro-Spin Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amount of cultured eukaryotic cells, tissues such as laser dissected samples (LDS) or fine needle aspirates (FNA). Normally, up to 5 x 105 eukaryotic cells or 5 mg tissue can be used in a single experiment. The kit allows single or multiple, simultaneous processing of samples in less than 30 mins, and purified RNA can be eluted with 10-15 µl water.

• Speed: RNA isolation in less than 30 mins • Safety: No organic extractions • Quality: Purified RNA suitable for any application • Lower elution volumes: RNA can be eluted with 10-15 µl water


Catalogo 2010-11


Prodotti Principali Omega Bio-Tek New high-throughput RNA with 96-well plate

The E-Z® Plant RNA Kit is designed for the automated high throughput isolation of total RNA from plant tissues.

• Speed: RNA isolation from 96 samples in less than 1 hr • Reliability: Optimized buffers guarantee high quality RNA everytime • Safety: No organic extractions • Quality: Purified RNA suitable for any application • High-throughput: Isolate RNA from 96 sample with 96-well plate

Purification of 2 mg RNA from 2-8 g plant samples

The E.Z.N.A.® Plant RNA Maxi Kit combines Omega Bio-Tek’s HiBind RNA Resin into Maxi-spin column format. It permits fast and efficient RNA purification form up to 2-8 g plant tissues in less than 40 mins. High quality plant total RNA can be reliably obtained from fresh or frozen samples. Purified RNA from this kit has O.D260/O.D280 rations of 1.8-2.0 and is suitable for downstream applications such as RT-PCR, Northern analysis, differential display, and poly A+ RNA selection. Purification mRNA From Total RNA or Culture Cells with magnetic beads

E.Z.N.A.® Magbeads® mRNA Kit offers a fast and efficient method to directly purify mRNA from total RNA. Cell or tissue samples are first lysed and homogenixed with RNAsafer Reagent. After a phenol/chloroform extraction, total RNA is precipitated with isopropanol. Redissolved total RNA sample is mixed with oligo (dT) magnetic particles which will capture the mRNA. After two simple wash step, mRNA can be eluted from the magnetic beads or the beads with mRNA can be directly used for RT-PCR. Simple and economic method for Isolation of genomic DNA from up to 1 ml blood

Compared with our traditional Blood kit (E.Z.N.A.® Blood DNA Kit), this system provides a fast and more economic method for the isolation of genomic DNA from whole blood by eliminating the use of Proteinase K. This method can also be used for the preparation of genomic DNA from buffy coat, serum, plasma and culture cells.

• Speed: Isolation DNA from 1 ml blood in less than 2 hrs • Safety: No organic extractions • Economic: Affordable for most lab • Quality: Purified RNA suitable for any application

RNA clean-up with lower elution volumes

The Micro-Spin RNA Clean-up Kit provides a rapid and easy method for the isolation and concentrate RNA from enzymatic reactions or for desalting RNA Samples. Up to 50 µg or down to picogram of RNA can be recovered from especially designed micro-spin RNA column.

• Speed: RNA isolation in less than 30 mins • Safety: No organic extractions • Quality: Purified RNA suitable for any application • Lower elution volumes: RNA can be eluted with 10-15 µl water

Purification high quality Genomic DNA from 500 mg animal tissues

The E.Z.N.A.® High performance (HP) Tissue DNA Maxi Kit is suitable for the isolation of genomic DNA from 500 mg of animal tissue or 1 x 108 cells. The special designed buffer system ensures the optimal lysis of tissues rich in fat, polysaccharides and fiber such as brain, adipose, muscles. The kit also can isolate DNA from molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides. Purification high quality Genomic DNA from 2 g animal tissues

The E.Z.N.A.® High performance (HP) Tissue DNA Maxi Kit is suitable for the isolation of genomic DNA from 2 g of animal tissue or 5 x 108 cells. The special designed buffer system ensures the optimal lysis of tissues rich in fat, polysaccharides and fiber such as brain, adipose, muscles. The kit also can isolate DNA from molluscs, insects, arthropods, roundworms, flatworms, and other invertebrate tissue samples rich in mucopolysaccharides.


Catalogo 2010-11


Prodotti Principali Omega Bio-Tek Vira Trap Adenovirus Purification kit

ViraTrap adenovirus purification Kit is a fast adenovirus purification method with high recovery.The adenovirus can be purified in 30 to 60 min. Compared to 24 hrs with traditional CsCl ultracentrifugation. This Kit provides 10 preparations and each preparation can produce up 3 x 10 VPs. The purified virus can then be used for the infection of cells and animal experiments.

Vira Trap AAV Purification kit

ViraTrap Adenovirus Associate Virus purification kit is a fast AAV purification methodwith high recovery. The AAV can be purified in 30 to 60 minutes. Compared to 24 hrs with traditional CsCl ultracentrifugation. The Kit provides 10 preparations and eachpreparation can produce up 3 x 10-12VPs. The purified virus can be used for infectionof the cell and animal experiments.

Vira Trap lentivirus and retrovirus Purification kit

ViraTrap lentivirus and retrovirus in 30 to 60 minutes. Compared to 24 hrs with traditional CsCl ultracentrifugation. The Kit provides 10 preparations and each preparation can produce up 3 x 10-12VPs. The purified virus can be used for infection of the cell and animal experiments.


Catalogo 2010-11


Kit per Plasmidi E.Z.N.A.® Plasmid DNA Isolation Omega Bio-Tek has greatly streamlined plasmid DNA purification with the innovative E.Z.N.A.® system. The marriage of time-tested alkaline-lysis and spin-column technology with proprietary HiBind® matrix allows convenient and reliable plasmid isolation. By rapidly and quantitatively binding DNA, HiBind® column eliminates many labor-intensive steps required in traditional plasmid purification procedures, including phenol-chloroform extractions, cesium chloride banding, alcohol precipitations, and slow gravity-flow ion-exchange columns. In addition, the membrane-like HiBind® matrix eliminates the common DNA shearing and silica resin carry-over observed in similar "bind-wash-elute" systems. As a result, large quantities of highly pure plasmid DNA can be isolated in very little time. The newly released E.Z.N.A.® Magbeads plasmid isolation kits provide another efficient technology to purify nucleic acids by using paramagnetic particles. Magnetic particles can be considered as a "mobile solid phase". The binding of nucleic acids to the magnetic particles occurs in solution, resulting in better binding kinetics and binding efficiency. This technology provides superior purity compared to most commercial nucleic acid purification kits because particles can be completely resuspended during the wash step of the purification protocol. Also new from OBI is the E.Z.N.A.® Fastfilter®. It is provided for large scale and endotoxin free plasmid kits using special filter units designed to replace centrifugation following the alkaline-lysis of bacterial cells. This filter cartridge completely removes SDS precipitates and clears bacterial lysates in a fraction of the time needed for centrifugation, reducing large scale plasmid purification time by up to 1 hour. Endotoxins are among the most common contaminants for plasmid preparation; the endotoxins have negative effects on downstream applications such as transfection. Omega Bio-Tek has released a few new products to provide a fast and economical way to prepare high purity and endotoxin free plasmid DNA. The V-spin column features an attached cap and a standard outlet luer that enables it to connect to a vacuum manifold. Compared to an ion-exchange column, the spin-column style Midi and Maxi columns provide a faster and easier method for large scale plasmid DNA isolations.

V-Spin Column


Catalogo 2010-11


Kit per Plasmidi: Selection Guide Standard Plasmid Isolation Kits

Descrizione DNA Purity Culture Volume Maxium Yield Code

E.Z.N.A.® Plasmid Mini Kit I High-Purity 1-5 ml 30 μg OME-D6942/3 E.Z.N.A.® Plasmid Mini Kit II High-Purity 3-15 ml 70 μg OME-D6945 E.Z.N.A.® X-Press Plasmid Mini Kit (<7min) High-Purity 1-3 ml 20 μg OME-D6947 E.Z.N.A.® Plasmid Midi Kit High-Purity 15-50 ml 200 μg OME-D6904 E.Z.N.A.® Plasmid Maxi Kit High-Purity 50-200 ml 1 mg OME-D6922 E.Z.N.A.® Plasmid Mega Kit Ultra-Pure 200-500 ml 2,5 mg OME-D6929 E.Z.N.A.® Plasmid Giga Kit Ultra-Pure 1000-2500 ml 10 mg OME-D6920 E.Z.N.A.® Fastfilter® Plasmid Midi Kit Ultra-Pure 15-50 ml 200 μg OME-D6905 E.Z.N.A.® Fastfilter® Plasmid Maxi Kit Ultra-Pure 50-200 ml 1 mg OME-D6924 E-Z 96® Fastfilter® Plasmid Kit Ultra-Pure 0,7-1,5 ml/well 12 μg/well OME-D1097 E-Z 96® SE Plasmid Kit (Simple-Economic) Ultra-Pure 0,7-1,5 ml/well 12 μg/well OME-D1095 E-Z 96® X-Press Plasmid Kit (<7min) High-Purity 0,7-1,5 ml/well 12 μg/well OME-D1047 E-Z 96® Swift Plasmid Kit (high-copy-plasmid DNA)

High-Purity 0,7-1,5 ml/well - OME-D1532

E.Z.N.A.® Yeast Plasmid Kit High-Purity 1-5 ml 5 μg OME-D3376 High Performance Plasmid Isolation Kits


E.Z.N.A.® HP Plasmid Mini Kit I Ultra-Pure 1-5 ml 30 μg OME-D7043 E.Z.N.A.® HP Plasmid Mini Kit II Ultra-Pure 3-15 ml 70 μg OME-D7045 E.Z.N.A.® HP Plasmid Midi Kit Ultra-Pure 15-50 ml 200 μg OME-D7004 E.Z.N.A.® HP Plasmid Maxi Kit Ultra-Pure 50-200 ml 1 mg OME-D7022

Endotoxin Free Plasmid Isolation Kits


E.Z.N.A.® Endo-Free Plasmid Mini Kit I Endotoxin-free 1-5 ml 30 μg OME-D6948 E.Z.N.A.® Endo-Free Plasmid Mini Kit II Endotoxin-free 3-15 ml 70 μg OME-D6950 E.Z.N.A.® Endo-Free Plasmid Midi Kit Endotoxin-free 15-50 ml 200 μg OME-D6915 E.Z.N.A.® Endo-Free Plasmid Maxi Kit Endotoxin-free 50-200 ml 1 mg OME-D6926 E.Z.N.A.® Endo-Free Plasmid Mega Kit Endotoxin-free 200-500 ml 2,5 mg OME-D6228 E.Z.N.A.® Endo-Free Plasmid Giga Kit Endotoxin-free 1000-2500 ml 10 mg OME-D6234

Magnetic Beads Plasmid Isolation Kit


E-Z 96® Mag-Bind Plasmid Mini Kit Ultra-Pure 1 ml/well 7 μg OME-M1256 E.Z.N.A.® Mag-Bind® Plasmid Mini Kit Ultra-Pure 1-5 ml 35 μg OME-M1260 E.Z.N.A.® Mag-Bind® Plasmid Maxi Kit Ultra-Pure 50-200 ml 1 mg OME-M1257 E.Z.N.A.® Mag-Bind® Plasmid Mega Kit Ultra-Pure 200-500 ml 5 mg OME-M1259 E-Z 96® Mag-Bind Endo-Free Plasmid Kit Endotoxin-free 1 ml/well 7 μg OME-M1258 E.Z.N.A.® Mag-Bind® Endo-Free Plasmid Maxi Kit Endotoxin-free 50-200 ml 1 mg OME-M1252 E.Z.N.A.® Mag-Bind® Endo-Free Plasmid Mega Kit Endotoxin-free 200-500 ml 5 mg OME-M1253

Large Construct DNA (PCAs, BACs, P1s, and Cosmid DNA Kit)


E.Z.N.A.® BAC/PAC DNA Kit Standard-Pure 1-5 ml/well - OME-D2156 E.Z.N.A.® BAC/PAC DNA Maxi Kit Ultra-Pure 500 ml - OME-D2154 E-Z 96® Fastfilter BAC/PAC DNA Kit Ultra-Pure 1,0 ml - OME-D1055


Catalogo 2010-11


Kit per Plasmidi Single-Strand Phage DNA Isolation Kits


E.Z.N.A.® M13 Isolation Kit Ultra-Pure 1-3 ml 15 μg OME-D6900 E-Z 96® M13 Isolation Kit Ultra-Pure 1-2 ml/well 15 μg OME-D1900 E.Z.N.A.® M13 Maxi Isolation Kit Ultra-Pure 500 ml 1-2 mg OME-D6908


Catalogo 2010-11


DNA Genomico: Selection Guide

Standard E.Z.N.A.® Spin Column and 96-Well format System

Descrizione Campione Applicazione E.Z.N.A.® Blood DNA Kit E.Z.N.A.® Blood DNA Midi Kit E.Z.N.A.® Blood DNA Maxi Kit E-Z 96® Blood DNA Kit E.Z.N.A.® NRBC Blood DNA Kit

Blood, liquid samples (<250 µl) Blood, liquid samples (<10 ml) Blood, liquid samples (<20 ml) Blood, liquid samples (<200 μl) Blood, liquid samples (<10 μl)

Purify 2-30 μg DNA from <250 µl blood, all liquid samples Purify 200-250 μg DNA from <10 ml blood, liquid samples Purify 1-1,2 mg DNA from <30 ml blood Purify 3-6 μg genomic DNA from <200 μl blood Purify 3-6 μg genomic DNA from <10 μl blood

E.Z.N.A.® Tissue DNA Kit

E.Z.N.A.® MicroElute Tissue DNA Kit

E.Z.N.A.® HP Tissue DNA Midi Kit

E.Z.N.A.® HP Tissue DNA Maxi Kit

E-Z 96® Tissue DNA Kit

Animal tissue and culture cells (<30 mg)

Tissue and forensic samples (<10 mg)


Animal tissue and culture cells (<2 g)


Purify 10-30 μg genomic DNA from tissues or cells

Purify DNA from small amounts of tissues or forensic samples

Purify 400-600 μg DNA from animal tissues or cells

Purify 1-1,2 mg DNA from animal tissues or cells

Purify 10 μg DNA from animal tissues or cells E.Z.N.A.® Forensic DNA Kit E.Z.N.A.® Mollusc DNA Kit E.Z.N.A.® Insect DNA Kit E.Z.N.A.® Stool DNA Kit E.Z.N.A.® Soil DNA Kit E.Z.N.A.® Yeast DNA Kit E.Z.N.A.® Bacterial DNA Kit E.Z.N.A.® Water DNA Kit

Dried Blood and forensic sample Mollusc, worm (<20 mg) Insect (<20 mg) Stool sample (<200 mg) Soil sample (200 mg - 0.5 g) 1x109 yeast culture 3 ml bacterial culture Water (<10 L)

Purify genomic DNA from dried blood, chewing gum, etc. Purify 10-50 μg genomic DNA from mollusc, worm samples Purify 10-50 μg genomic DNA from insect samples Purify genomic DNA from stool samples Purify genomic DNA from soil samples Purify 10-30 μg genomic DNA from yeast culture Purify 10-50 μg genomic DNA from bacterial culture Purify genomic DNA from water samples

E.Z.N.A.® Plant DNA Kit

E.Z.N.A.® Plant DNA Midi Kit

E.Z.N.A.® Plant DNA Maxi Kit

E-Z 96® Plant DNA Kit

Plant cells or tissues (<100 mg)


Plant cells or tissues (<2 g)


Purify 8-40 μg genomic DNA from fresh or dry plant samples


Purify 600 μg-2,5 mg genomic DNA from fresh or dry plant samples

Purify 10-25 μg DNA from fresh or dry plant samples E.Z.N.A.® SP Plant DNA Kit

E.Z.N.A.® SP Plant DNA Midi Kit

E.Z.N.A.® SP Plant DNA Maxi Kit



Plant cells or tissues (<1 g)



Purify 1-2,5 mg genomic DNA from fresh or dry plant samples E.Z.N.A.® HP Plant DNA Kit E.Z.N.A.® HP Plant DNA Midi Kit E.Z.N.A.® HP Plant DNA Maxi Kit

Plant cells or tissues (<100 mg) Plant cells or tissues (<500 mg) Plant cells or tissues (<2 g)

Purify 10-50 μg genomic DNA from fresh or dry plant samples Purify genomic DNA from fresh or dry plant samples Purify genomic DNA from fresh or dry plant samples

E.Z.N.A.® Fungal DNA Kit

E.Z.N.A.® Fungal DNA Midi Kit

E.Z.N.A.® Fungal DNA Maxi Kit

E-Z 96® Fungal DNA Kit

Fungal cells or tissues (<100 mg)


Fungal cells or tissues (<2 g)


Purify 10-40 μg genomic DNA from fresh or dry fungal samples

Purify 200-250 μg genomic DNA from fresh or dry fungal samples

Purify 1-2 mg genomic DNA from fresh or dry fungal samples

Purify 10-20 μg DNA from fresh or dry fungal samples E.Z.N.A.® SP Fungal DNA Kit E.Z.N.A.® SP Fungal DNA Midi Kit E.Z.N.A.® HP Fungal DNA Kit

Fungal cells or tissues (<100 mg) Fungal cells or tissues (<500 mg) Fungal cells or tissues (<100 mg)

Purify 30-60 μg DNA from fresh or dry fungal samples Purify DNA from fresh or dry fungal samples Purify DNA from fresh or dry fungal samples

High Molecular Weight Genomic DNA System

Descrizione Campione Applicazione

E.Z.N.A.® SQ Blood DNA Kit I Blood < 20 ml Purify high molecular weight genomic DNA from blood

E.Z.N.A.® SQ Blood DNA Kit II Blood < 20 ml Purify high molecular weight genomic DNA from blood

E.Z.N.A.® SQ NRBC Blood DNA Kit Blood < 400 µl Purify high molecular weight genomic DNA from blood

E.Z.N.A.® SQ Tissue DNA Kit Animal tissue and cells Purify high molecular weight genomic DNA from tissue or cells

E.Z.N.A.® SQ Plant DNA Kit Plant tissue or cells Purify high molecular weight genomic DNA from plant tissue or cells


Catalogo 2010-11


DNA Genomico: Selection Guide

Paramagnetic Beads System

Descrizione Campione Applicazione E.Z.N.A.® Mag-Bind® Blood DNA Mini Kit E.Z.N.A.® Mag-Bind® Blood Large Scale Kit E.Z.N.A.® Mag-Bind® Blood DNA KF 96 Kit E.Z.N.A.® Mag-Bind® Blood DNA Midi Kit E.Z.N.A.® Mag-Bind® Blood DNA Maxi Kit E-Z 96® Mag-Bind® Blood DNA Micro Kit E-Z 96® Mag-Bind® Blood DNA Mini Kit E-Z 96® Mag-Bind® Blood DNA Midi Kit

Blood, all liquid samples (<250 μl) Blood, all liquid samples (<12 ml) Blood, all liquid samples (<200 µl) Blood, all liquid samples (<2 ml) Blood, all liquid samples (<10 ml) Blood, all liquid samples (<100 μl) Blood, all liquid samples (100-250 µl) Blood, all liquid samples (300-350 µl)

Purify genomic DNA from blood with magnetic beads Purify genomic DNA from <12 ml blood with magnetic beads Purify genomic DNA from <200 µl blood with magnetic beads Purify genomic DNA from <2 ml blood with magnetic beads Purify genomic DNA from <10 ml blood with magnetic beads Purify genomic DNA from <100 µl blood with magnetic beads Purify genomic DNA from 100-250 µl blood w\ith magnetic beads Purify genomic DNA from 300-350 µl blood with magnetic beads

E.Z.N.A.® Mag-Bind® Tissue DNA Kit E-Z 96® Mag-Bind® Tissue DNA Kit E.Z.N.A.® Mag-Bind® Tissue DNA KF 96 Kit E.Z.N.A.® Mag-Bind® Forensic DNA Kit E-Z 96® Mag-Bind® Forensic DNA Kit E.Z.N.A.® Mag-Bind® Forensic DNA KF96Kit

Animal tissue and culture cells (<10 mg) Animal tissue and culture cells (<10 mg) Animal tissue and culture cells (<10 mg) Forensic case work samples Forensic samples (<20 mg) Forensic samples (<20 mg)

Purify genomic DNA from animal tissue with magnetic beads Purify genomic DNA from animal tissue with magnetic beads Purify genomic DNA from animal tissue with magnetic beads Purify DNA from forensic case samples with magnetic beads Purify DNA from forensic case samples with magnetic beads Purify DNA from forensic case samples with magnetic beads

E.Z.N.A.® Mag-Bind® Soil DNA Kit E.Z.N.A.® Mag-Bind® Soil DNA 96 Kit

Soil samples (200 mg) Soil samples (200 mg)

Purify DNA from soil samples with magnetic beads Purify DNA from soil samples with magnetic beads

E.Z.N.A.® Mag-Bind® Stool DNA Kit Stool samples (<20 mg) Purify DNA from stool samples with magnetic beads

E.Z.N.A.® Mag-Bind® Plant DNA Kit

E-Z 96® Mag-Bind® Plant DNA Kit

E.Z.N.A.® Mag-Bind® Plant DNA Maxi Kit

Plant tissues or cells (<100 mg)

Plant tissues or cells (<100 mg)

Plant tissues or cells (<5 g)

Purify genomic DNA from plant tissues with magnetic beads

Purify genomic DNA from plant tissues with magnetic beads

Purify genomic DNA from <5 g plant tissues with magnetic beads


Catalogo 2010-11


Kit per RNA: Selection Guide E.Z.N.A.® RNA Isolation and Purification Manipulation of RNA is inherently more challenging than that of DNA since RNase is not only ubiquitous to most laboratories, it is also extremely unstable. Omega Bio-Tek’s E.Z.N.A.® RNA kits allow for the reliable isolation of RNA from a variety of sources with the HiBind® spin-column method. These systems involve the simultaneous lysis of cells and the inactivation of endogenous RNases. This is accomplished by highly denaturing buffers that enhance the reversible binding of RNA to the HiBind® matrix. While cells can be lysed directly, tissues are disrupted or homogenized using common laboratory supplies. Binding conditions are further adjusted and lysates are applied to spin columns that selectively bind RNA molecules greater than 200 nt in length, thus favoring mRNA isolation. Successive washes with specially formulated buffers remove inhibitors and contaminants, allowing subsequent elution of pure RNA with water. As with other members of the E.Z.N.A.® family of products, phenol and chloroform are eliminated for safety, convenience, and speed. Kits are tailor-made for specific cells and tissue sources in order to ensure consistent and reliable RNA preps--every time. Standard E.Z.N.A.® RNA Spin Column and 96-Well format System

Descrizione Campione Column Type/

RNA Binding Capacity Average Yield

(μg) E.Z.N.A.® Blood RNA Kit E.Z.N.A.® Blood RNA Midi Kit E.Z.N.A.® Blood RNA Maxi Kit E.Z.N.A.® X-Press Blood RNA Kit

Whole Blood (up to 1 ml) Whole Blood (up to 10 ml) Whole Blood (up to 50 ml) Whole Blood (up to 150 ml)

Mini/100 μg Midi/1 mg Maxi/5 mg

X-Press/10 µg

1-7 μg 10-70 μg 50-350 μg 0,5-1 μg

E.Z.N.A.® Total RNA Kit I

E-Z 96® Total RNA Kit

E-Z 96® Total RNA Kit II

E.Z.N.A.® Total RNA Midi Kit

E.Z.N.A.® Total RNA Maxi Kit

E.Z.N.A.® Micro-Elute® Total RNA Kit

1x107 eukaryotic cells/30 mg tissue


All types of tissue (up to 10 mg)


5x108 eukaryotic cells/1 g tissue

<1x105 eukaryotic cells/5 mg tissue

Mini/100 μg

100 µg/well

100 µg/well

Midi/1 mg

Maxi/5 mg

Micro/50 μg

20-70 μg

5-20 μg

20-90 μg

0,5-1 mg

1-5 mg

1-30 μg E.Z.N.A.® Total RNA Kit II

E.Z.N.A.® Tissue RNA Kit

E-Z 96® Tissue RNA Kit

All types of sample (up to 100 mg)

Fibrous tissue and other difficult to lyse samples

Fibrous tissue and other difficult to lyse samples

Mini/100 μg

Mini/100 μg

100 μg/well

30-90 μg

5-15 μg

5-10 μg

E.Z.N.A.® Mollusc RNA Kit Molluscs, Arthropods, roundworms, flatworms and other invertebrate tissue samples Mini/10 μg 10-40 μg

E.Z.N.A.® Plant RNA Kit

E.Z.N.A.® Plant RNA Midi Kit

E.Z.N.A.® Plant RNA Maxi Kit

E-Z 96® Plant RNA Kit

Plant cells or tissues (100 mg)

Plant cells or tissues (500 mg)

Plant cells or tissues (2-5 g)

Plant cells or tissues (10-50 mg/well)

Mini/100 μg

Midi/1 mg

Maxi/5 mg

100 μg/well

10-60 μg

100-500 μg

0,5-2 mg

5-20 μg E.Z.N.A.® Fungal RNA Kit Fungal cells or tissues (100 mg) Mini/100 μg 10-30 μg E.Z.N.A.® Yeast RNA Kit Yeast (up to 5x107 cells) Mini/100 μg 10-50 μg E.Z.N.A.® Bacterial RNA Kit Bacterial (up to 1x109) Mini/100 μg 20-60 μg E.Z.N.A.® Soil RNA Kit Soil (up to 2 g) Mini/100 μg 2-10 μg E.Z.N.A.® Viral RNA Kit

E-Z 96® Viral RNA Kit Serum, urine, plasma and other cell free body fluids

Mini/100 μg

100 μg/well

<10 μg

<10 μg

E.Z.N.A.® Ultra Pure Total RNA Midi Kit

E.Z.N.A.® Ultra Pure Total RNA Maxi Kit

All types of sample (up to 200 mg)

All types of sample (up to 1 g)

Midi/1 mg

Maxi/5 mg 0,5-1 mg

1-5 mg E.Z.N.A.® FFPE RNA Kit

E-Z 96® FFPE RNA Kit

Formalin-Fixed Paraffin-Embedded samples(20mg)

Formalin-Fixed Paraffin-Embedded samples(10mg) 100 μg

100 μg/well

1-5 μg

5-10 μg

E.Z.N.A.® Paramagnetic Bead System



(μg) E.Z.N.A.® Mag-Bind® Total RNA Kit

E.Z.N.A.® Mag-Bind® Viral DNA/RNA Kit

E.Z.N.A.® Mag-Bind® mRNA Enrichment Kit

E.Z.N.A.® Mag-Bind® mRNA Kit E.Z.N.A.® Mag-Bind® DNA/RNA/Protein Kit


Serum, Plasma, Cell Free Body Fluids Total RNA or RNA enzymatic reactions

Total RNA or tissue -

-

-

-

- -

5-50 μg

<10 µg 2-20 µg 2-20 µg

-

E.Z.N.A.® Total DNA/RNA Isolation System and 96-Well format System



(μg) E.Z.N.A.® Total DNA/RNA Kit E-Z 96® Total DNA/RNA Kit E.Z.N.A.® HP Viral DNA/RNA Kit E-Z 96® HP Viral DNA/RNA Kit E.Z.N.A.® Micro-Elute Viral DNA/RNA Kit E.Z.N.A.® SQ DNA/RNA/Protein Kit

1x107 eukaryotic cells/30 mg tissue 1x107 eukaryotic cells/10 mg tissue Serum, Plasma, Cell Free Body Fluids Serum, Plasma, Cell Free Body Fluids Serum, Plasma, Cell Free Body Fluids Any samples

100 μg 100 μg/well

100 μg 100 μg/well

50 μg -

10-70 µg 5-20 µg <10 µg <10 µg <10 µg

-


Catalogo 2010-11


Kit per RNA: Selection Guide Others



(μg) E.Z.N.A.® RNA-Safer® Reagent E.Z.N.A.® RNA-Solv® Reagent E.Z.N.A.® RNA-Solv® Reagent Concentrate E.Z.N.A.® SQ Total RNA Kit

Variable, all types of tissue 1x107 eukaryotic cells/100 mg tissue 1x107 eukaryotic cells/100 mg tissue Variable, all types of tissue

- - - -

- 10-200 µg 10-200 µg

-


Catalogo 2010-11


Kit purificazione DNA/RNA: Selection Guide E.Z.N.A.® DNA/RNA Clean-Up System HiBind® Spin column technology and HiBind® Magbeads® technology have been developed for many protocols, and are now available for DNA and RNA recovery and clean-up from a variety of sources. Under appropriate conditions, nucleic acids can be purified from reaction mixtures and agarose or acrylamide gels. Regardless of the application and type of nucleic acid (labeled or not), there are E.Z.N.A® Clean-up Kits available for rapid and reliable purification. E.Z.N.A.® Clean-Up kits are:

• Fast: Most procedures are completed in less than 20 minutes • Reliable: HiBind® technology means high purity and high yields every time • Safe: No organic extractions required • Simple: Detailed but easy-to-follow instructions eliminate confusion • Flexible: Most kits can be adapted for different situations

Standard E.Z.N.A.® Spin Column System

Descrizione Size Recovery Applicazione

E.Z.N.A.® Cycle-Pure Kit 100 bp-10 kb 85% PCR product purification

E.Z.N.A.® Gel Extraction Kit 70 bp-20 kb 85% DNA recovery from agarose gel

E.Z.N.A.® Poly-Gel DNA Extraction Kit 100 bp-10 kb n/a DNA recovery from poly-acrylamide gel

E.Z.N.A.® DNA Probe PurificationKit 100 bp-10 kb 85% DNA Clean-Up from enzymatic reactions

E.Z.N.A.® RNA Probe Purification Kit n/a 85-90% RNA Clean-Up from enzymatic reactions

E.Z.N.A.® Poly-Gel RNA Extraction Kit n/a n/a RNA recovery from poly-acrylamide gel

E.Z.N.A.® MicroElute® Column System


E.Z.N.A.® MicroElute® Cycle-Pure Kit 100 bp-10 kb 85% PCR product purification special column for lower elution volume (10-15 μl)

E.Z.N.A.® MicroElute® Gel Extraction Kit 70 bp-20 kb 85% Recover DNA from agrose gel special column for lower elution volume (10-15 μl)

E.Z.N.A.® MicroElute® DNA Clean-Up Kit 100 bp-10 kb 85% Recover DNA from enzymatic reactions special column for lower elution volume (10-15 μl)

E.Z.N.A.® MicroElute® RNA Clean-Up Kit n/a 85-90% Recover RNA from enzymatic reactions special column for lower elution volume (10-15 μl)

96-Well Format Purification System


E-Z 96® Cycle-Pure Kit 100 bp-10 kb 85% PCR product purification in a 96-well format E-Z 96® Ultra-Sep® Dye Terminator Removal Kit n/a n/a Dye Terminator Removal in a 96-well format

Silica Beads Based System


Ultra-Sep® Gel Extraction Kit

60 bp-200 bp 200 bp – 1 kb

1-5 kb 25-50 kb

<60% 85% 85% 80%

Low cost DNA recovery from agarose gel

Ultra-Sep® Dye Terminator Removal Kit n/a n/a Dye Terminator Removal


Catalogo 2010-11


Kit purificazione DNA/RNA: Selection Guide

Paramagnetic Beads System


E-Z 96® Mag-Bind® Cycle-Pure Kit 100 bp-10 kb 90-95% PCR product purification with magnetic beads

E.Z.N.A.® Mag-Bind® Oligonucleotide Purification Kit

10 bp-100 bp 60-75% Oligonucleotide Purification with magnetic beads

E-Z 96® Mag-Bind® Oligonucleotide Purification Kit

10 bp-100 bp 60-75% Oligonucleotide Purification with magnetic beads in a 96-well format

E.Z.N.A.® Mag-Bind® Poly-Gel DNA Extraction Kit <100bp n/a DNA recovery from poly-acrylamide gel with magnetic beads

E-Z 96® Mag-Bind® Dye Terminator Removal Kit n/a n/a Dye Terminator Removal with magnetic beads in a 96-well format

E.Z.N.A.® Mag-Bind® RNA Clean-Up Kit n/a 85-90% Recover RNA from enzymatic reactions with magnetic beads in a 96-well format


Catalogo 2010-11


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